Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates

Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control ( 220 ± 150 vs. 18 ± 9 pg / ml , P < 0.001 ), ( 316 ± 198 vs. 23.7 ± 14 pg / ml , P < 0.001 ), ( 78.2 ± 34 vs. 3.3 ± 1.7 mg / L , P < 0.001 ), ( 11.2 ± 0.9 vs. 8.6 ± 0.4 fL ), and ( 7.08 ± 3.3 vs. 0.4 ± 0.2 , P < 0.001 ), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff value ≥ 9.2 fL . CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff value > 0.9 . Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates.

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Salivary Interleukin-6 and C-Reactive Protein/Mean Platelet Volume Ratio in the Diagnosis of Late-Onset Neonatal Pneumonia

Journal of Immunology Research ◽

10.1155/2021/8495889 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Ahmed Omran ◽

Yasmin Ali ◽

Mohamed Osama Abdalla ◽

Sonya El-Sharkawy ◽

Ahmed R. Rezk ◽

...

Keyword(s):

Interleukin 6 ◽

Predictive Value ◽

Mean Platelet Volume ◽

Late Onset ◽

Case Fatality ◽

Full Term ◽

C Reactive Protein ◽

Platelet Volume ◽

Term Neonates ◽

Reactive Protein

Neonatal pneumonia is a serious respiratory infectious disease with a high rate of case fatality in developing countries. Salivary cytokines could serve as interesting noninvasive markers in the diagnosis of neonatal pneumonia. The aim was to assess the diagnostic role of salivary and serum interleukin-6 (IL-6), C-reactive protein/mean platelet volume (CRP/MPV) ratio, and the combination of these markers in the diagnosis of late-onset neonatal pneumonia in full-term neonates. Seventy full-term neonates, 35 with late-onset neonatal pneumonia and 35 controls, were enrolled in this prospective case-control study. Complete blood count (CBC), salivary and serum IL-6, and CRP concentrations were measured for all the study subjects. The sensitivity, specificity, positive predictive value, and negative predictive value of salivary IL-6, serum IL-6, and CRP/MPV ratio for the diagnosis of late-onset neonatal pneumonia were determined. At the cutoff point of >34 pg/ml, salivary IL-6 showed 82.86% sensitivity and 91.43% specificity. CRP/MPV ratio showed a sensitivity of 97.14% and specificity of 85.71% at a cutoff value > 0.88 . The combination of salivary IL-6 and CRP/MPV ratio improved the sensitivity and specificity to 100%. The current study shows for the first time that both salivary IL-6 and CRP/MPV ratio are suitable markers for the diagnosis of late-onset neonatal pneumonia in full-term neonates.

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Salivary C-reactive protein, mean platelet volume and neutrophil lymphocyte ratio as diagnostic markers for neonatal sepsis

Jornal de Pediatria ◽

10.1016/j.jped.2017.03.006 ◽

2018 ◽

Vol 94 (1) ◽

pp. 82-87 ◽

Cited By ~ 17

Author(s):

Ahmed Omran ◽

Abdallah Maaroof ◽

Mai H.S. Mohammad ◽

Amina Abdelwahab

Keyword(s):

Neonatal Sepsis ◽

Mean Platelet Volume ◽

Diagnostic Markers ◽

C Reactive Protein ◽

Platelet Volume ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

Reactive Protein

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Diagnostic Value of Urine sTREM-1 and Urine C-reactive Protein for Infants with Late Onset Neonatal Sepsis

Journal of Pediatric Infectious Diseases ◽

10.1055/s-0039-3400468 ◽

2019 ◽

Vol 15 (02) ◽

pp. 072-078

Author(s):

Senem Alkan Ozdemir ◽

Ruya Colak ◽

Ezgi Yangin Ergon ◽

Sebnem Calkavur

Keyword(s):

Neonatal Sepsis ◽

Predictive Value ◽

Late Onset ◽

Diagnostic Value ◽

C Reactive Protein ◽

Reactive Protein ◽

Late Onset Sepsis ◽

Noninvasive Markers ◽

Serum Crp ◽

Sepsis Detection

Abstract Objective Noninvasive markers have been increasingly used as a diagnostic marker for sepsis detection and monitoring of the disease. The aim of this observational, prospective pilot study was to investigate the diagnostic performance of urinary soluble triggering receptor expressed on myeloid cells (sTREM-1) and urine C-reactive protein (CRP) levels in the late onset neonatal sepsis and to compare them with serum CRP levels. Materials and Methods Sixty-six infants with clinical sepsis were included. Urine sTREM-1 and urine CRP were collected at the diagnosis of late-onset sepsis. All laboratory investigations were also noted from the infants. Results There were no significant differences between characteristics of the infants. Culture-positive neonates had significantly higher urine sTREM-1 than culture-negative neonates (p < 0.001). Using a cut-off point for urine sTREM-1 of 129 pg/mL, the sensitivity was 0.63, the specificity was 0.84, positive predictive value was 0.80, negative predictive value was 0.70. Urine sTREM-1 and urine CRP were recollected on the seventh day of sepsis treatment and it was found that the levels of sTREM-1 and CRP decreased. Conclusion This is the first study in the literature which evaluates the place of urine sTREM-1 and urine CRP in the diagnosis of neonatal sepsis. Urine sTREM-1 and urine CRP may be useful in the diagnosis of sepsis and in evaluating the effect of antibiotic treatment.

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Salivary C‐reactive protein, mean platelet volume and neutrophil lymphocyte ratio as diagnostic markers for neonatal sepsis

Jornal de Pediatria (Versão em Português) ◽

10.1016/j.jpedp.2017.07.004 ◽

2018 ◽

Vol 94 (1) ◽

pp. 82-87 ◽

Cited By ~ 1

Author(s):

Ahmed Omran ◽

Abdallah Maaroof ◽

Mai H.S. Mohammad ◽

Amina Abdelwahab

Keyword(s):

Neonatal Sepsis ◽

Mean Platelet Volume ◽

Diagnostic Markers ◽

C Reactive Protein ◽

Platelet Volume ◽

Neutrophil Lymphocyte Ratio ◽

Lymphocyte Ratio ◽

Reactive Protein

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Association between DNA Damage and Serum Levels of Copper, Zinc, and Selenium in Full-Term Neonates with Late-Onset Sepsis

Journal of Pediatric Infectious Diseases ◽

10.1055/s-0040-1717126 ◽

2020 ◽

Vol 15 (06) ◽

pp. 299-306

Author(s):

Aliaa Ahmed Wahby ◽

Maha Elwassif ◽

Mai Magdy ◽

Tamer A. Abdel Hamid ◽

Alshaymaa A. Ibrahim

Keyword(s):

Risk Factors ◽

Dna Damage ◽

Trace Elements ◽

Neonatal Sepsis ◽

Late Onset ◽

Serum Levels ◽

Full Term ◽

Assay Method ◽

Term Neonates ◽

Late Onset Sepsis

Abstract Objective The alteration in certain trace elements is usually associated with impaired immune function and higher oxidative stress. Therefore, these elements are suggested to play an important role in the pathogenesis of neonatal sepsis. We aimed to evaluate copper (Cu), zinc (Zn), and selenium (Se) serum levels in full-term neonates with late-onset sepsis (LOS) and correlate these levels with DNA damage and other risk factors of sepsis. Methods The study included a group of 100 neonates diagnosed with sepsis serving as the case group and another one of 60 neonates serving as the control group. DNA damage was assessed using the comet assay method and trace elements were measured using inductively coupled plasma mass spectrometry. Results Compared with controls, the percentage of DNA damage was significantly elevated in patients with sepsis, while serum levels of Cu, Zn, and Se were markedly decreased (p = 0.001). A strong negative correlation was revealed between Se and DNA damage (r = −0.6, p = 0.001). However, no correlations were found between Cu or Zn and DNA damage. Univariate logistic regression analysis revealed that DNA damage as well as Cu, Zn, and Se serum levels can be considered as relevant risk factors for neonatal sepsis (p = 0.008, 0.004, 0.004, and 0.003, respectively). Receiver-operating characteristic curve analysis showed that the strongest indicator for neonatal sepsis was Se (area under the curve [AUC] = 0.94, confidence interval [CI] = 0.9–0.98, p = 0.001), followed by Cu (AUC = 0.9, CI = 0.85–0.96, p = 0.001), and then Zn (AUC = 0.87, CI = 0.8–0.93, p = 0.001). Conclusion The percentage of DNA damage may help in the assessment of neonatal sepsis severity. Altered levels of Cu, Zn, and Se may play significant role in the pathogenesis of neonatal sepsis. Se serum level is strongly correlated with percentage of DNA damage. Therefore, Se can predict the severity of LOS.

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