Salivary Interleukin-6 and C-Reactive Protein/Mean Platelet Volume Ratio in the Diagnosis of Late-Onset Neonatal Pneumonia

Neonatal pneumonia is a serious respiratory infectious disease with a high rate of case fatality in developing countries. Salivary cytokines could serve as interesting noninvasive markers in the diagnosis of neonatal pneumonia. The aim was to assess the diagnostic role of salivary and serum interleukin-6 (IL-6), C-reactive protein/mean platelet volume (CRP/MPV) ratio, and the combination of these markers in the diagnosis of late-onset neonatal pneumonia in full-term neonates. Seventy full-term neonates, 35 with late-onset neonatal pneumonia and 35 controls, were enrolled in this prospective case-control study. Complete blood count (CBC), salivary and serum IL-6, and CRP concentrations were measured for all the study subjects. The sensitivity, specificity, positive predictive value, and negative predictive value of salivary IL-6, serum IL-6, and CRP/MPV ratio for the diagnosis of late-onset neonatal pneumonia were determined. At the cutoff point of >34 pg/ml, salivary IL-6 showed 82.86% sensitivity and 91.43% specificity. CRP/MPV ratio showed a sensitivity of 97.14% and specificity of 85.71% at a cutoff value > 0.88 . The combination of salivary IL-6 and CRP/MPV ratio improved the sensitivity and specificity to 100%. The current study shows for the first time that both salivary IL-6 and CRP/MPV ratio are suitable markers for the diagnosis of late-onset neonatal pneumonia in full-term neonates.

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Salivary and Serum Interleukin-10, C-Reactive Protein, Mean Platelet Volume, and CRP/MPV Ratio in the Diagnosis of Late-Onset Neonatal Sepsis in Full-Term Neonates

Journal of Immunology Research ◽

10.1155/2021/4884537 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Ahmed Omran ◽

Hazem Sobh ◽

Mohamed Osama Abdalla ◽

Sonya El-Sharkawy ◽

Ahmed R. Rezk ◽

...

Keyword(s):

Neonatal Sepsis ◽

Mean Platelet Volume ◽

Late Onset ◽

Interleukin 10 ◽

Full Term ◽

C Reactive Protein ◽

Platelet Volume ◽

Term Neonates ◽

Reactive Protein ◽

Late Onset Sepsis

Salivary markers could serve as potential noninvasive markers in the diagnosis of neonatal infections. We aimed to investigate the diagnostic role of salivary and serum interleukin 10 (IL-10), C-reactive protein (CRP), mean platelet volume (MPV), and CRP/MPV ratio in the diagnosis of late-onset neonatal sepsis in full-term neonates. Seventy full-term neonates were enrolled in this prospective case-control study, 35 with late-onset neonatal sepsis, and 35 controls. Salivary IL-10, serum IL-10, and CRP concentrations were measured by enzyme-linked immunosorbent assay (ELISA). Complete blood (CBC) count was measured by an automated blood cell counter. The salivary IL-10, serum IL-10, CRP, MPV, and CRP/MPV ratio levels were much higher in neonates with late-onset sepsis than in control ( 220 ± 150 vs. 18 ± 9 pg / ml , P < 0.001 ), ( 316 ± 198 vs. 23.7 ± 14 pg / ml , P < 0.001 ), ( 78.2 ± 34 vs. 3.3 ± 1.7 mg / L , P < 0.001 ), ( 11.2 ± 0.9 vs. 8.6 ± 0.4 fL ), and ( 7.08 ± 3.3 vs. 0.4 ± 0.2 , P < 0.001 ), respectively. At the cutoff point of >31 pg/ml, salivary IL-10 showed 97.1% sensitivity and 94.3% specificity. Serum IL-10 at a cutoff value of ≥33.6 pg/ml had a sensitivity of 97.1% and specificity of 80%. MPV showed a sensitivity of 100% and specificity of 94.4% at a cutoff value ≥ 9.2 fL . CRP/MPV ratio showed a sensitivity of 100% and specificity of 97.1% at a cutoff value > 0.9 . Salivary and serum IL-10 showed a positive correlation with CRP and CRP/MPV ratio in septic neonates. The current study shows for the first time that both salivary IL-10 and CRP/MPV showed statistically significant differences between neonates with late-onset sepsis and controls. Accordingly, salivary IL-10 could serve as a potential noninvasive biomarker for the diagnosis of late-onset sepsis in full-term neonates.

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Salivary C‐reactive protein and mean platelet volume in diagnosis of late‐onset neonatal pneumonia

The Clinical Respiratory Journal ◽

10.1111/crj.12723 ◽

2017 ◽

Vol 12 (4) ◽

pp. 1644-1650 ◽

Cited By ~ 6

Author(s):

Ahmed Omran ◽

Mohammed Ali ◽

Mai H.S. Mohammad ◽

Osama Zekry

Keyword(s):

Mean Platelet Volume ◽

Late Onset ◽

C Reactive Protein ◽

Platelet Volume ◽

Reactive Protein

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The association between serum YKL-40 levels, mean platelet volume, and c-reactive protein in patients with cellulitis

Indian Journal of Medical Microbiology ◽

10.4103/0255-0857.150891 ◽

2015 ◽

Vol 33 (5) ◽

pp. 61 ◽

Cited By ~ 7

Author(s):

A Erturk ◽

E Cure ◽

MC Cure ◽

E Parlak ◽

A Kurt ◽

...

Keyword(s):

Mean Platelet Volume ◽

C Reactive Protein ◽

Platelet Volume ◽

Reactive Protein

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C-reactive protein and interleukin-6 responses for differentiating fungal and bacterial aetiology in late-onset neonatal sepsis

Mycoses ◽

10.1111/j.1439-0507.2009.01802.x ◽

2011 ◽

Vol 54 (3) ◽

pp. 212-216 ◽

Cited By ~ 11

Author(s):

Suna Serife Oguz ◽

Erden Sipahi ◽

Ugur Dilmen

Keyword(s):

Interleukin 6 ◽

Neonatal Sepsis ◽

Late Onset ◽

C Reactive Protein ◽

Reactive Protein ◽

Bacterial Aetiology

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The cut-off levels of procalcitonin and C-reactive protein and the kinetics of mean platelet volume in preterm neonates with sepsis

BMC Pediatrics ◽

10.1186/s12887-018-1236-2 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 4

Author(s):

C. Aydemir ◽

H. Aydemir ◽

F. Kokturk ◽

C. Kulah ◽

A. G. Mungan

Keyword(s):

Mean Platelet Volume ◽

Preterm Neonates ◽

C Reactive Protein ◽

Platelet Volume ◽

Reactive Protein ◽

Kinetics Of

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Diagnostic Value of Neutrophil to Lymphocyte Ratio and Mean Platelet Volume on Early Onset Neonatal Sepsis on Term Neonate

Journal of Pediatric Intensive Care ◽

10.1055/s-0040-1715104 ◽

2020 ◽

Author(s):

Birol Karabulut ◽

Silem Ozdem Alatas

Keyword(s):

Neonatal Sepsis ◽

Predictive Value ◽

Mean Platelet Volume ◽

Early Onset ◽

Area Under The Curve ◽

Neutrophil To Lymphocyte Ratio ◽

Control Group ◽

Platelet Volume ◽

Term Neonates ◽

Lymphocyte Ratio

AbstractBy setting out from increased neutrophil count, decreased lymphocyte count, and increased mean platelet volume (MPV), which is a result of the effect of inflammation on blood cells, we aimed to investigate whether neutrophil to lymphocyte ratio (NLP) and MPV can be used as an auxiliary parameter for the diagnosis of early-onset neonatal sepsis (EOS). This study was conducted by analyzing term neonates with EOS and physiological jaundice who were admitted to the neonatal intensive care unit of Izmir Katip Celebi University Ataturk Training and Research Hospital. A total of 63 neonate files were examined to include 30 term neonates with EOS, and 77 neonate files were examined to include 30 term neonates with physiological jaundice as a control group. NLR had an area under the curve (AUC) of 0.891 for prediction of EOS. At a cut-off level of 1.42, NLR had a likelihood ratio (LR) of 5.5, sensitivity of 88%, a specificity of 84%, a positive predictive value (PPV) of 84.6%, and a negative predictive value (NPV) of 87.5%. MPV had an AUC of 0.666 for the prediction of EOS and at a cut-off level of 9.3 fL, MPV had an LR of 1.23, sensitivity of 84%, a specificity of 32%, a PPV of 55.2%, and an NPV of 66.6%. In conclusion, this study provides evidence that NLR and MPV can be used in addition to conventional parameters in the diagnosis of EOS.

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