scholarly journals Deep Learning Method for RNA Secondary Structure Prediction with Pseudoknots Based on Large-Scale Data

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Bowen Shen ◽  
Hao Zhang ◽  
Cong Li ◽  
Tianheng Zhao ◽  
Yuanning Liu
Keyword(s):  
Deep Learning ◽  
Secondary Structure ◽  
Structure Prediction ◽  
Rna Secondary Structure ◽  
Large Scale ◽  
Secondary Structure Prediction ◽  
Learning Methods ◽  
Rna Secondary Structure Prediction ◽  
Large Scale Data ◽  
Scale Data

Traditional machine learning methods are widely used in the field of RNA secondary structure prediction and have achieved good results. However, with the emergence of large-scale data, deep learning methods have more advantages than traditional machine learning methods. As the number of network layers increases in deep learning, there will often be problems such as increased parameters and overfitting. We used two deep learning models, GoogLeNet and TCN, to predict RNA secondary results. And from the perspective of the depth and width of the network, improvements are made based on the neural network model, which can effectively improve the computational efficiency while extracting more feature information. We process the existing real RNA data through experiments, use deep learning models to extract useful features from a large amount of RNA sequence data and structure data, and then predict the extracted features to obtain each base’s pairing probability. The characteristics of RNA secondary structure and dynamic programming methods are used to process the base prediction results, and the structure with the largest sum of the probability of each base pairing is obtained, and this structure will be used as the optimal RNA secondary structure. We, respectively, evaluated GoogLeNet and TCN models based on 5sRNA, tRNA data, and tmRNA data, and compared them with other standard prediction algorithms. The sensitivity and specificity of the GoogLeNet model on the 5sRNA and tRNA data sets are about 16% higher than the best prediction results in other algorithms. The sensitivity and specificity of the GoogLeNet model on the tmRNA dataset are about 9% higher than the best prediction results in other algorithms. As deep learning algorithms’ performance is related to the size of the data set, as the scale of RNA data continues to expand, the prediction accuracy of deep learning methods for RNA secondary structure will continue to improve.

scholarly journals RNA secondary structure prediction using deep learning with thermodynamic integration

2020 ◽  
Author(s):  
Kengo Sato ◽  
Manato Akiyama ◽  
Yasubumi Sakakibara
Keyword(s):  
Deep Learning ◽  
Secondary Structure ◽  
Structure Prediction ◽  
Rna Secondary Structure ◽  
Secondary Structure Prediction ◽  
Secondary Structures ◽  
Thermodynamic Integration ◽  
Rna Secondary Structure Prediction ◽  
Rna Secondary Structures ◽  
Non Coding Rnas

RNA secondary structure prediction is one of the key technologies for revealing the essential roles of functional non-coding RNAs. Although machine learning-based rich-parametrized models have achieved extremely high performance in terms of prediction accuracy, the risk of overfitting for such models has been reported. In this work, we propose a new algorithm for predicting RNA secondary structures that uses deep learning with thermodynamic integration, thereby enabling robust predictions. Similar to our previous work, the folding scores, which are computed by a deep neural network, are integrated with traditional thermodynamic parameters to enable robust predictions. We also propose thermodynamic regularization for training our model without overfitting it to the training data. Our algorithm (MXfold2) achieved the most robust and accurate predictions in computational experiments designed for newly discovered non-coding RNAs, with significant 2–10 % improvements over our previous algorithm (MXfold) and standard algorithms for predicting RNA secondary structures in terms of F-value.

scholarly journals UFold: Fast and Accurate RNA Secondary Structure Prediction with Deep Learning

2020 ◽  
Author(s):  
Yingxin Cao ◽  
Laiyi Fu ◽  
Jie Wu ◽  
Qing Nie ◽  
Xiaohui Xie
Keyword(s):  
Deep Learning ◽  
Secondary Structure ◽  
Structure Prediction ◽  
Rna Secondary Structure ◽  
Secondary Structure Prediction ◽  
Correction Function ◽  
Thermodynamic Models ◽  
Rna Secondary Structure Prediction ◽  
Rna Molecules ◽  
Online Web

AbstractFor many RNA molecules, the secondary structure is essential for the correction function of the RNA. Predicting RNA secondary structure from nucleotide sequences is a long-standing problem in genomics, but the prediction performance has reached a plateau over time. Traditional RNA secondary structure prediction algorithms are primarily based on thermodynamic models through free energy minimization. Here we propose a deep learning-based method, called UFold, for RNA secondary structure prediction, trained directly on annotated data without any thermodynamic assumptions. UFold improves substantially upon previous models, with approximately 31% improvement over traditional thermodynamic models and 24.5% improvement over other learning-based methods. It achieves an F1 score of 0.96 on base pair prediction accuracy. An online web server running UFold is publicly available at http://ufold.ics.uci.edu.

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