Abstract 1587: Loss of LZAP inactivates p53 in head and neck cancer and regulates sensitivity of cells to DNA damage in the p53-dependent manner.

Aim of the study. To assess the role of genes RAD50 and SMARCA5 in regulation of sensitivity to cisplatin and other anticancer DNA damaging drugs (5-fluorouracil, olaparib), to assess the role of these genes in the response to the DNA damage in ovarian cancer (OVCAR-8) and head and neck cancer cell lines (SCC61, SCC25). Material and Methods. We used small interfering RNAs (siRNAs) (Qiagen,Germany) to deplete either RAD50 and SMARCA5 or control genes and under basal and DNA damaging drug-treatment conditions, we assessed cell viability with the use of Cell Titer Blue reagent (Promega,USA) with following spectrophotometry. To assess the role of genes in response to DNA damage, analysis of phospho-H2AX focus formation was performed by means of using immunofluorescence microscopy. Results. We demonstrated the role of RAD50 and SMARCA5 in regulation of survival and sensitivity of ovarian cancer (OVCAR-8), head and neck cancer cell lines (SCC61, SCC25) to cisplatin and other DNA damaging drugs (5-fluorouracil, olaparib). Our data suggest the role of SMARCA5 in regulation of baseline histone H2AX protein phosphorylation in the absence of DNA damage. Conclusion. Our findings characterize genes RAD50 and SMARCA5 as promising therapeutic targets and predictive markers for response to cisplatin and other DNA damaging drugs in patients with ovarian cancer and squamous cell carcinoma of head and neck.

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Abstract 5425: Potent curcumin analogue FLLL-22, but not natural curcumin, targets the DNA damage pathway for apoptosis in lung and head and neck cancer cells

10.1158/1538-7445.am2011-5425 ◽

2011 ◽

Author(s):

A.R.M. Ruhul Amin ◽

Michelle Lee ◽

Mohammad A. Rahman ◽

James R. Fuchs ◽

Zhuo G. Chen ◽

...

Keyword(s):

Dna Damage ◽

Head And Neck Cancer ◽

Head And Neck ◽

Cancer Cells ◽

Neck Cancer ◽

Curcumin Analogue

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Abstract 4895: P16INK4A, a surrogate marker of HPV infection and prognosis for head and neck cancer, delays DNA damage repair and enhances radiation response

10.1158/1538-7445.am2014-4895 ◽

2014 ◽

Author(s):

Li Wang ◽

Peijing Zhang ◽

David P Molkentine ◽

Hailong Piao ◽

Chunyan Chen ◽

...

Keyword(s):

Dna Damage ◽

Head And Neck Cancer ◽

Head And Neck ◽

Neck Cancer ◽

Dna Damage Repair ◽

Surrogate Marker ◽

Hpv Infection ◽

Radiation Response ◽

Damage Repair

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DNA Damage and Repair of Head and Neck Cancer Cells after Radio- and Chemotherapy

Zeitschrift für Naturforschung C ◽

10.1515/znc-2009-7-821 ◽

2009 ◽

Vol 64 (7-8) ◽

pp. 601-610 ◽

Cited By ~ 8

Author(s):

Pawel Rusin ◽

Anna Walczak ◽

Anita Zwierzchlejska ◽

Jurek Olszewski ◽

Alina Morawiec-Bajda ◽

...

Keyword(s):

Dna Damage ◽

Dna Repair ◽

Head And Neck Cancer ◽

Head And Neck ◽

Cancer Cells ◽

Neck Cancer ◽

Cancer Cell Line ◽

Repair Capacity ◽

Dna Damage And Repair ◽

Γ Radiation

DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and γ-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to γ-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy.

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