Ca2+-Dependent Histaminergic Regulation of Proenkephalin mRNA Levels in Cultured Adrenal Chromaffin Cells

1987 ◽  
Vol 46 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Nikolai Kley ◽  
Jean Philippe Loeffler ◽  
Volker Höllt
2002 ◽  
Vol 73 (4) ◽  
pp. 1749-1757 ◽  
Author(s):  
Toshihiko Yanagita ◽  
Hideyuki Kobayashi ◽  
Ryuichi Yamamoto ◽  
Yasunari Takami ◽  
Hiroki Yokoo ◽  
...  

Author(s):  
Joe A. Mascorro ◽  
Robert D. Yates

Extra-adrenal chromaffin organs (abdominal paraganglia) constitute rich sources of catecholamines. It is believed that these bodies contain norepinephrine exclusively. However, the present workers recently observed epinephrine type granules in para- ganglion cells. This report investigates catecholamine containing granules in rabbit paraganglia at the ultrastructural level.New Zealand white rabbits (150-170 grams) were anesthetized with 50 mg/kg Nembutal (IP) and perfused with 3% glutaraldehyde buffered with 0.2M sodium phosphate, pH 7.3. The retroperitoneal tissue blocks were removed and placed in perfusion fluid for 4 hours. The abdominal paraganglia were dissected from the blocks, diced, washed in phosphate buffer and fixed in 1% osmic acid buffered with phosphate. In other animals, the glutaraldehyde perfused tissue blocks were immersed for 1 hour in 3% glutaraldehyde/2.5% potassium iodate buffered as before. The paraganglia were then diced, separated into two vials and washed in the buffer. A portion of this tissue received osmic acid fixation.


1997 ◽  
Vol 73 ◽  
pp. 226
Author(s):  
Kazuo Minakuchi ◽  
Hitoshi Houchi ◽  
Masanori Yoshizumi ◽  
Yasuko Ishimura ◽  
Kyoji Morita ◽  
...  

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