basic fibroblast growth factor
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Author(s):  
Bo Fu ◽  
Xiaobei Wang ◽  
Zhengda Chen ◽  
Nan Jiang ◽  
Zhigang Guo ◽  
...  

Myocardial infarction (MI) has been considered as the leading cause of cardiovascular-related deaths worldwide. Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factors that promotes angiogenesis...


Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1762
Author(s):  
Leah R. Benington ◽  
Gunesh Rajan ◽  
Cornelia Locher ◽  
Lee Yong Lim

Basic fibroblast growth factor (FGF-2) is a highly labile protein with strong potential for tissue engineering. The aim of this study was to develop FGF-2 formulations that are stable against physical stressors encountered in pharmaceutical processing and evaluation. Pharmaceutical excipients, alone or in combination, were added to aqueous FGF-2 (770 ng/mL) solution and the stability of the resulting solutions on storage at 4–37 °C was evaluated. Stability of the solutions to repeated freeze-thaw cycles and lyophilisation was also evaluated, as well as the stability of the lyophilised stabilised protein to storage at −4, 4 and 18 °C for up to 12 months. In all of these experiments FGF-2 was quantified by ELISA assay. The as-received FGF-2, when dissolved in water, was highly unstable, retaining only 50% of baseline protein content after 30 min at 37 °C or 1 h at 25 °C. By contrast, FGF-2 solutions prepared with 0.5% w/v methylcellulose (MC) and 20 mM alanine (formulation F5) or with 0.5% w/v MC and 1 mg/mL human serum albumin (HSA) (formulation F6) were highly stable, having residual FGF-2 content comparable to baseline levels even after 2 h at 37 °C and 5 h at 25 °C. F5 and F6 were also highly stable to repeated freeze-thaw cycles, with >99% of FGF-2 load remaining after the third cycle. In addition, F5 and F6 were stable to lyophilisation, and the lyophilised products could be stored at −4, 4 or 18 °C for at least 12 months, with less than 1% loss in mean FGF-2 content. Thus, FGF-2 solution is effectively stabilised against both thermal and processing stressors in the presence of MC and alanine (F5), or MC and HSA (F6). The resultant FGF-2 solutions may be applied as medicinal products or further processed into more advanced medicinal products, e.g., scaffolds, for wound healing and tissue regeneration.


2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Takeshi Kataoka ◽  
Yutaka Mifune ◽  
Atsuyuki Inui ◽  
Hanako Nishimoto ◽  
Takashi Kurosawa ◽  
...  

Abstract Introduction Excellent outcomes of arthroscopic rotator cuff repair for small and medium tears have been recently reported. However, re-tears after surgery have been a common complication after surgical repair of large and massive rotator cuff tears and often occur in early postoperative phase. It was previously reported that basic fibroblast growth factor and platelet-rich plasma enhanced rotator cuff tear healing. We hypothesized that this combined therapy could enhance rotator cuff healing after rotator cuff repair in a rat model. This study aimed to evaluate the efficacy of combined therapy of platelet-rich plasma and basic fibroblast growth factor with gelatin-hydrogel sheet. Methods To create a rotator cuff defect, the infraspinatus tendon of Sprague Dawley rat was resected from the greater tuberosity. The infraspinatus tendons were repaired and covered with gelatin-hydrogel sheet impregnated with PBS (control group), basic fibroblast growth factor (bFGF group), platelet-rich plasma (PRP group), or both basic fibroblast growth factor and platelet-rich plasma (combined group). Histological examinations were conducted using hematoxylin and eosin, safranin O, and immunofluorescence staining, such as Isolectin B4, type II collagen at 2 weeks postoperatively. For mechanical analysis, ultimate failure load of the tendon-humeral head complex was evaluated at 6 weeks postoperatively. Results In the hematoxylin and eosin staining, the tendon maturing score of the combined group was higher than that of the control group at postoperative 2 weeks. In the safranin O staining, stronger proteoglycan staining was observed in the combined group compared with the other groups at postoperative 2 weeks. Vascular staining with isolectin B4 in 3 treatment groups was significantly higher than that in the control group. Type II collagen expression in the combined group was significantly higher than those in the other groups. The ultimate failure load of the combined group was significantly higher than that of the control group. Conclusion Combined therapy of basic fibroblast growth factor and platelet-rich plasma promoted angiogenesis, tendon maturing and fibrocartilage regeneration at the enthesis, which could enhance the mechanical strength. It was suggested that combined basic fibroblast growth factor and platelet-rich plasma might enhance both tendon and bone–tendon junction healing, and basic fibroblast growth factor and platelet-rich plasma might be synergistic.


Respiration ◽  
2021 ◽  
pp. 1-5
Author(s):  
Shuliang Guo ◽  
Yang Bai ◽  
Yishi Li ◽  
Tao Chen

A large central bronchopleural fistula (BPF) surrounded by mediastinal tissue was successfully closed by local administration of recombinant bovine basic fibroblast growth factor (rbFGF) using the bronchoscope. No complications were observed during and after this bronchoscopic treatment. This is the first report of the bronchoscopic treatment of a large central BPF by the local spray of rbFGF. The bronchoscopic treatment with rbFGF is a potentially cost-effective method for central BPF surrounded by mediastinal tissue.


2021 ◽  
Author(s):  
Takeshi Kataoka ◽  
Yutaka Mifune ◽  
Atsuyuki Inui ◽  
Hanako Nishimoto ◽  
Takashi Kurosawa ◽  
...  

Abstract Introduction: Excellent outcomes of arthroscopic rotator cuff repair for small and medium tears have been recently reported. However, re-tears after surgery have been a common complication after surgical repair of large and massive rotator cuff tears andoften occur in early postoperative phase. It was previously reported that basic fibroblastgrowth factor and platelet-rich plasma enhanced rotator cuff tear healing. Wehypothesized that this combined therapy could enhance rotator cuff healing after rotatorcuff repair in a rat model. This study aimed to evaluate the efficacy of combined therapyof platelet-rich plasma and basic fibroblast growth factor with gelatin-hydrogel sheet.Methods: To create a rotator cuff defect, the infraspinatus tendon of Sprague Dawley ratwas resected from the greater tuberosity. The infraspinatus tendons were repaired andcovered with gelatin-hydrogel sheet impregnated with PBS (control group), basicfibroblast growth factor (bFGF group), platelet-rich plasma (PRP group), or both basicfibroblast growth factor and platelet-rich plasma (combined group). Histologicalexaminations were conducted using hematoxylin and eosin, safranin O, andimmunofluorescence staining such as Isolectin B4, type II collagen. For mechanicalanalysis, ultimate failure load of the tendon-humeral head complex was evaluated at41 weeks postoperatively.Results: In the hematoxylin and eosin staining, the tendon maturing score of the combined group was higher than that of the control group postoperativeat 2 weeks. Inthe safranin O staining, stronger proteoglycan staining was observed in the combinedgroup compared with the other groups at postoperative 2 weeks. Vascular staining withisolectin B4 in 3 treatment groups was significantly higher than that in the control group.Type II collagen expression in the combined group was significantly higher than those inthe other groups. The ultimate failure load of the combined group was significantly higherthan that of the control group.Conclusion: Combined therapy of basic fibroblast growth factor and platelet-rich plasmapromoted angiogenesis; tendon maturing and fibrocartilage regeneration at the enthesis,which could be enhance the mechanical strength. It was suggested that basic fibroblastgrowth factor and platelet-rich plasma enhance both tendon and bone-tendon junctionhealing, and basic fibroblast growth factor and platelet-rich plasma were synergistic.


2021 ◽  
Author(s):  
Song Xue ◽  
Fan Zhou ◽  
Tian Zhao ◽  
Huimin Zhao ◽  
Xuewei Wang ◽  
...  

Liquid-liquid phase separation (LLPS) driven by weak, multivalent interactions among biomolecules is an important means of cellular compartmentation and plays a central role in cellular processes including stress resistance, RNA processing and other cellular activities. Coordination of the condensates and inner membrane was recently revealed, mediating intracellular processes like cell signalling and cargo trafficking. Intracellular LLPS has been observed extensively in vivo, whereas LLPS in extracellular compartments has not been reported under physiological conditions. Here we show, for the first time, that basic fibroblast growth factor (bFGF) undergoes LLPS on the cell surface by interacting with heparan sulphate proteoglycans (HSPG) and the phase transition is required for effective downstream signalling. The condensation is driven by multivalent interactions between bFGF and sulpho- groups on heparan sulphate (HS), and dimerization and oligomerization of bFGF promote the LLPS process. Compared with free bFGF, phase separated bFGF with HS showed higher thermo stability, providing a potential mechanism for the preservation of bFGF activity. Furthermore, we have found that downstream signalling is triggered by phase separation of a ternary complex formed by bFGF, HSPGs and FGFR on cell surface. Our results revealed a molecular mechanism that HS can serve as a platform to promote extracellular proteins like bFGF to condensate on outer membrane, consequently coordinating the signal transduction activities. This novel finding expands the horizons of phase separation in vivo, providing a new dimension on how HSPG may regulate extracellular protein behaviour and cell signalling.


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