Effects of Prednisolone and Human Epidermal Growth Factor on Angiogenesis in Granulation Tissue of Gastric Ulcer Induced by Acetic Acid

Digestion ◽  
1989 ◽  
Vol 42 (3) ◽  
pp. 135-142 ◽  
Author(s):  
Satoshi Hase ◽  
Saburo Nakazawa ◽  
Yoshihisa Tsukamoto ◽  
Kose Segawa
Keyword(s):  
Acetic Acid ◽  
Gastric Ulcer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Granulation Tissue ◽  
Human Epidermal Growth Factor ◽  
Epidermal Growth

scholarly journals Protective Effect of Ocotillol, the Derivate of Ocotillol-Type Saponins in Panax Genus, against Acetic Acid-Induced Gastric Ulcer in Rats Based on Untargeted Metabolomics

2020 ◽  
Vol 21 (7) ◽  
pp. 2577 ◽  
Author(s):  
Cuizhu Wang ◽  
Yuze Yuan ◽  
He Pan ◽  
Alan Chen-Yu Hsu ◽  
Jinluan Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acetic Acid ◽  
Gastric Ulcer ◽  
Growth Factor ◽  
Gastric Mucosa ◽  
Epidermal Growth Factor ◽  
Serum Levels ◽  
Gastroprotective Effect ◽  
Epidermal Growth ◽  
Oxide Synthase

Gastric ulcer (GU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Finding a natural drug with a gastroprotective effect is needed. Ocotillol, the derivate of ocotillol-type saponins in the Panax genus, possesses good anti-inflammatory activity. The study aimed to investigate the gastroprotective effect of ocotillol on acetic acid-induced GU rats. The serum levels of endothelin-1 (ET-1) and nitric oxide (NO), the gastric mucosa levels of epidermal growth factor, superoxide dismutase and NO were assessed. Hematoxylin and eosin staining of gastric mucosa for pathological changes and immunohistochemical staining of ET-1, epidermal growth factor receptors and inducible nitric oxide synthase were evaluated. A UPLC-QTOF-MS-based serum metabolomics approach was applied to explore the latent mechanism. A total of 21 potential metabolites involved in 7 metabolic pathways were identified. The study helps us to understand the pathogenesis of GU and to provide a potential natural anti-ulcer agent.

scholarly journals Expression, Microencapsulation of Recombinant Human Epidermal Growth Factor, and Release Study in Gastric Ulcer Representing Condition

2021 ◽  
pp. 493-502
Author(s):  
Riyona Desvy Pratiwi
Keyword(s):  
Gastric Ulcer ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Expression Systems ◽  
E Coli ◽  
Human Epidermal Growth Factor ◽  
Chitosan Microparticles ◽  
Proliferation Activity ◽  
Epidermal Growth ◽  
External Wound

Recombinant human epidermal growth factor (rhEGF) has been studied and expressed in various expression systems. It has been also commercialized and clinically used, yet limited to topical diseases. However, being naturally expressed in different tissues, the rhEGF is potential to be applied not only for external wound and skin disorders, but also to regenerates internal damaged epidermal cells such found in gastric ulcer. In the recent study, chitosan microparticles were developed to facilitate delivery of the rhEGF and to overcome gastric degradation that majorly interfere protein, particularly rhEGF oral administration. The rhEGF was expressed in E. coli BL21(DE3) and purified using Ni-NTA chromatography. The refolded rhEGF showed proliferation activity on MC7 cells. rhEGF loaded chitosan microparticles were stable in the gastric and specifically released the loaded rhEGF in the high oxidative environment in acidic pH representing gastric ulcer condition.    

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