scholarly journals Lactic Acid Bacteria as a Surface Display Platform for Campylobacter jejuni Antigens

2015 ◽  
Vol 25 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Patrycja Kobierecka ◽  
Agnieszka Wyszyńska ◽  
Marta Maruszewska ◽  
Anna Wojtania ◽  
Joanna Żylińska ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Campylobacter Jejuni ◽  
Surface Display ◽  
Food Poisoning ◽  
Lactobacillus Salivarius ◽  
Binding Ability ◽  
Hybrid Proteins ◽  
Mucosal Delivery ◽  
Delivery Vehicles

<b><i>Background:</i></b> Food poisoning and diarrheal diseases continue to pose serious health care and socioeconomic problems worldwide. <i>Campylobacter</i> spp. is a very widespread cause of gastroenteritis. Over the past decade there has been increasing interest in the use of lactic acid bacteria (LAB) as mucosal delivery vehicles. They represent an attractive opportunity for vaccination in addition to vaccination with attenuated bacterial pathogens. <b><i>Methods:</i></b> We examined the binding ability of hybrid proteins to nontreated or trichloroacetic acid (TCA)-pretreated LAB cells by immunofluorescence and Western blot analysis. <b><i>Results:</i></b> In this study we evaluated the possibility of using GEM (Gram-positive enhancer matrix) particles of <i>Lactobacillus salivarius</i> as a binding platform for 2 conserved, immunodominant, extracytoplasmic <i>Campylobacter jejuni</i> proteins: CjaA and CjaD. We analyzed the binding ability of recombinant proteins that contain <i>C. jejuni</i> antigens (CjaA or CjaD) fused with the protein anchor (PA) of the <i>L. lactis </i>peptidoglycan hydrolase AcmA, which comprises 3 LysM motifs and determines noncovalent binding to the cell wall peptidoglycan. Both fused proteins, i.e. 6HisxCjaAx3LysM and 6HisxCjaDx3LysM, were able to bind to nontreated or TCA-pretreated <i>L. salivarius</i> cells. <b><i>Conclusion:</i></b> Our results documented that the LysM-mediated binding system allows us to construct GEM particles that present 2 <i>C. jejuni</i> antigens.

Lactic acid bacteria as mucosal delivery vehicles: a realistic therapeutic option

2016 ◽  
Vol 100 (13) ◽  
pp. 5691-5701 ◽  
Author(s):  
Miao Wang ◽  
Zeqian Gao ◽  
Yongguang Zhang ◽  
Li Pan
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Therapeutic Option ◽  
Mucosal Delivery ◽  
Delivery Vehicles

scholarly journals A SH3_5 Cell Anchoring Domain for Non-recombinant Surface Display on Lactic Acid Bacteria

2021 ◽  
Vol 8 ◽  
Author(s):  
Pei Kun Richie Tay ◽  
Pei Yu Lim ◽  
Dave Siak-Wei Ow
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Surface Display ◽  
Bioactive Molecules ◽  
Gastric Digestion ◽  
Functional Protein ◽  
Fluorescent Reporter ◽  
Surface Conditions ◽  
Bacterial Surface

Lactic acid bacteria (LAB) are a group of gut commensals increasingly recognized for their potential to deliver bioactive molecules in vivo. The delivery of therapeutic proteins, in particular, can be achieved by anchoring them to the bacterial surface, and various anchoring domains have been described for this application. Here, we investigated a new cell anchoring domain (CAD4a) isolated from a Lactobacillus protein, containing repeats of a SH3_5 motif that binds non-covalently to peptidoglycan in the LAB cell wall. Using a fluorescent reporter, we showed that C-terminal CAD4a bound Lactobacillus fermentum selectively out of a panel of LAB strains, and cell anchoring was uniform across the cell surface. Conditions affecting CAD4a anchoring were studied, including temperature, pH, salt concentration, and bacterial growth phase. Quantitative analysis showed that CAD4a allowed display of 105 molecules of monomeric protein per cell. We demonstrated the surface display of a functional protein with superoxide dismutase (SOD), an antioxidant enzyme potentially useful for treating gut inflammation. SOD displayed on cells could be protected from gastric digestion using a polymer matrix. Taken together, our results show the feasibility of using CAD4a as a novel cell anchor for protein surface display on LAB.

scholarly journals Screening of Indigenous Oxalate Degrading Lactic Acid Bacteria from Human Faeces and South Indian Fermented Foods: Assessment of Probiotic Potential

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Sivasamy Gomathi ◽  
Ponnusamy Sasikumar ◽  
Kolandaswamy Anbazhagan ◽  
Sundaresan Sasikumar ◽  
Murugan Kavitha ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Fermentum ◽  
Fermented Foods ◽  
Lactobacillus Salivarius ◽  
Human Faeces ◽  
South Indian ◽  
Rdna Sequencing ◽  
Acid And Bile Tolerance

Lactic acid bacteria (LAB) have the potential to degrade intestinal oxalate and this is increasingly being studied as a promising probiotic solution to manage kidney stone disease. In this study, oxalate degrading LAB were isolated from human faeces and south Indian fermented foods, subsequently assessed for potential probiotic propertyin vitroandin vivo. Based on preliminary characteristics, 251 out of 673 bacterial isolates were identified as LAB. A total of 17 strains were found to degrade oxalate significantly between 40.38% and 62.90% and were subjected to acid and bile tolerance test. Among them, nine strains exhibited considerable tolerance up to pH 3.0 and at 0.3% bile. These were identified asLactobacillus fermentumandLactobacillus salivariususing 16S rDNA sequencing. Three strains,Lactobacillus fermentumTY5,Lactobacillus fermentumAB1, andLactobacillus salivariusAB11, exhibited good adhesion to HT-29 cells and strong antimicrobial activity. They also conferred resistance to kanamycin, rifampicin, and ampicillin, but were sensitive to chloramphenicol and erythromycin. The faecal recovery rate of these strains was observed as 15.16% (TY5), 6.71% (AB1), and 9.3% (AB11) which indicates the colonization ability. In conclusion, three efficient oxalate degrading LAB were identified and their safety assessments suggest that they may serve as good probiotic candidates for preventing hyperoxaluria.

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