scholarly journals Eficácia do tratamento de sementes com o nematicida tioxazafen no controle dos nematoides Heterodera glycines, Meloidogyne incognita, M. javanica, Pratylenchus brachyurus e P. zeae

2016 ◽  
Author(s):  
Elvio Lorençato Uzuele
Keyword(s):  
Meloidogyne Incognita ◽  
Heterodera Glycines ◽  
Pratylenchus Brachyurus

scholarly journals Tagetes minuta Propagation and Interaction With Nematoide

2018 ◽  
Vol 11 (1) ◽  
pp. 139
Author(s):  
Camila T. Stroze ◽  
Fernando C. Baida ◽  
Maria I. Balbi-Peña ◽  
Cláudia R. Dias-Arieira ◽  
Débora C. Santiago
Keyword(s):  
Meloidogyne Incognita ◽  
Heterodera Glycines ◽  
Nematode Species ◽  
Root Anatomy ◽  
Plant Parasite ◽  
Tagetes Minuta ◽  
Pratylenchus Brachyurus ◽  
Rf Values ◽  
Nematode Penetration ◽  
Reproduction Factor

Tagetes minuta is a plant presenting pest and disease control potential, although its activity on some plant parasite nematode species is poorly investigated. The aim of the current study is to evaluate the best T. minuta propagation way, as well as the plant reaction to nematodes such as Heterodera glycines, Meloidogyne incognita and Pratylenchus brachyurus, through nematode penetration and reproduction studies and root morphological assessments. Seedlings obtained from woody cuttings showed larger rooting and stem diameter. Heterodera glycines penetration was higher than that recorded for M. incognita. However, both species recorded reproduction factor (RF) lower than one, as well as maximum RF values 0.34 and 0.02 for H. glycines and M. incognita, respectively. The highest FR recorded for T. minuta were found in P. brachyurus (0.96 and 0.80) in the 60th and 80th day after inoculation (DAI). Root anatomy changes were observed in the 80th DAI in plants inoculated with P. brachyurus. Tagetes minuta was resistant to all nematodes; however, it did not prevent P. brachyurus activity in its roots.

scholarly journals Comparação entre Técnicas de Coloração de Raízes de Soja Infectadas por Heterodera glycines, Meloidogyne incognita e Pratylenchus brachyurus

2018 ◽  
Vol 1 (1) ◽  
pp. 100
Author(s):  
Fernando Godinho de Araújo ◽  
Kássia Aparecida Garcia Barbosa ◽  
Leonardo De Castro Santos ◽  
Renato Andrade Teixeira ◽  
Mara Rubia da Rocha
Keyword(s):  
Meloidogyne Incognita ◽  
Heterodera Glycines ◽  
Pratylenchus Brachyurus

Conduziu-se experimento em casa de vegetação para avaliar a eficiência de duas técnicas de coloração de raízes de soja cultivar Emgopa 313, infectadas pelos três principais fitonematóides que afetam a cultura: Heterodera glycines, Meloidogyne incognita e Pratylenchus brachyurus. Foram realizadas três avaliações aos 10, 17 e 25 dias após o plantio da soja em solo naturalmente infestado, com os nematóides avaliados. A técnica empregando clareamento com NaOCl e fucsina ácida e a técnica que emprega lacto-glicerol e fucsina ácida foram eficientes na coloração dos nematóides, permitindo a sua visualização no interior do tecido radicular. Em função da duração do ciclo de vida do P. brachyurus não foi possível a visualização desses nematóides nos tecidos radiculares na primeira avaliação empregando a técnica do clareamento e coloração com fucsina ácida, e na segunda avaliação, em ambas as técnicas testadas. Para tecidos mais tenros a técnica que emprega o clareamento seria a mais indicada por facilitar a visualização dos nematóides, já que os tecidos sofrem certa descoloração, e por utilizar uma menor quantidade de corante, o que auxilia no processo de descoloração.

scholarly journals Nematóides associados a plantas invasoras

1976 ◽  
Vol 33 (0) ◽  
pp. 597-615 ◽  
Author(s):  
Antonio Carlos Zem ◽  
Luiz Gonzaga E. Lordello
Keyword(s):  
Meloidogyne Incognita ◽  
Meloidogyne Javanica ◽  
Meloidogyne Arenaria ◽  
Pratylenchus Brachyurus ◽  
Pratylenchus Zeae

O presente trabalho trata de nematóides associados a raízes de 71 plantas invasoras, nas quais revelou-se a presença de 8 gêneros e 18 espécies parasitas. As espécies, pela ordem decrescente de freqüência foram: Helicotylenchus dihystera (46%) ; Meloidogyne javanica (24%) ; Meloidogyne incognita (23%); Macroposthonia ornata (17%); Pratylenchus brachyurus (16%) ; Pratylenchus zeae (14%) ; Xiphinemaseiariae (11%); Xiphinema krugi (10%); Helicotylenchus pseudorobustus (5,6%); Meloidogyne arenaria (4,2%); Helicotylenchus dUiysteroides (4,2%) ; Tylenchorhynchus martini (4,2%) ; Aphelenchoides sp. (2,8%) ; Ditylenchus sp. (2,8%) ; Helicotylerfichus microcephalus (2,8%) ; Xiphinema elongatum (2,8%) ; Helicotylenchus egyptiensis (1,4%) e Meloidogyne coffeicola (1,4%). São feitas considerações sobre as implicações para a agricultura de tão ampla distribuição de nematóides em plantas colonizadoras e gramíneas utilizadas como forrageiras.

scholarly journals KEEFEKTIFAN BAKTERI ENDOFIT UNTUK MENGENDALIKAN NEMATODA Pratylenchus brachyurus PADA TANAMAN NILAM

2020 ◽  
Vol 17 (1) ◽  
pp. 6
Author(s):  
RITA HARNI ◽  
SUPRAMANA SUPRAMANA ◽  
MEITY S. SINAGA ◽  
GIYANTO GIYANTO ◽  
SUPRIADI SUPRIADI
Keyword(s):  
Meloidogyne Incognita ◽  
Endophytic Bacteria ◽  
Penetration Rate ◽  
Achromobacter Xylosoxidans ◽  
Radopholus Similis ◽  
Fresh Weight ◽  
Greenhouse Condition ◽  
Pogostemon Cablin ◽  
Pratylenchus Brachyurus ◽  
Uninoculated Control

<p>ABSTRAK</p><p>Penggunaan bakteri endofit sebagai agen pengendalian nematodaparasit seperti Meloidogyne incognita pada kapas dan tomat, Globoderasp. pada kentang dan Radopholus similis pada pisang telah banyak ditelitipada beberapa jenis tanaman. Penelitian bertujuan untuk menganalisiskeefektifan  beberapa  bakteri  endofit terhadap  perkembangan  P.brachyurus, penetrasi, reproduksi, dan kerusakan yang diakibatkannyapada tanaman nilam. Penelitian dilakukan di Laboratorium dan Rumahkaca Hama dan Penyakit Balai Penelitian Tanaman Obat dan AromatikBogor, dari bulan Maret sampai Agustus 2008. Penelitian menggunakanrancangan acak lengkap (RAL) 6 perlakuan dengan 7 ulangan. Lima isolatbakteri endofit, yaitu Achromobacter xylosoxidans TT2, Alcaligenesfaecalis NJ16, Pseudomonas putida EH11, Bacillus cereus MSK, danBacillus subtilis NJ57, diaplikasikan pada setek tanaman nilam denganmetode perendaman akar. Seminggu setelah tanam, nilam diinokulasidengan 500 ekor P. brachyurus. Pengamatan dilakukan terhadap penetrasi,reproduksi, populasi nematoda, dan pertumbuhan nilam. Hasil penelitianmenunjukkan bahwa bakteri endofit A. xylosoxidans TT2, A. faecalisNJ16, P. putida EH11, B. cereus MSK, dan B. subtilis NJ57 dapatmenekan penetrasi dan populasi P. brachyurus ke dalam akar sebesar54,8-70,6% dengan faktor reproduksi (pf/pi) 0,61-0,94 dan meningkatkanpertumbuhan tanaman nilam sebesar 37,86-84,71%.</p><p>Kata kunci: Pogostemon cablin, bakteri endofit, keefektifan, nematoda,Pratylenchus brachyurus, pengendalian biologi</p><p>ABSTRACT</p><p>Effectiveness  of  endophytic  bacteria  to  controlPratylenchus brachyurus nematode on patchouli</p><p>The use of endophytic bacteria as biocontrol agents for nematodes,such as Meloidogyne incognita on cotton and tomatoes, Globodera sp. onpotatoes and Radopholus similis on bananas has been widely studied inseveral crops. The aim of the study was to investigate the effectiveness ofsome endophytic bacteria to control P. brachyurus, penetration,reproduction, and plant fresh weight production. Five isolates, namely A.xylosoxidans TT2, A. faecalis NJ16, P. putida EH11, B. cereus MSK, andB. subtilis NJ57 were applied to the patchouli cutting roots by soakingmethod before planting. A week after planting, the plants were inoculatedwith 500 juveniles and adults of P. brachyurus. Observations were doneon penetration and reproduction rates of the nematode, and growth ofpatchouli plant. Under greenhouse condition, A. xylosoxidans TT2, A.faecalis NJ16, P. putida EH11, B. cereus MSK, and B. subtilis NJ57reduced penetration rate of P. brachyurus into the patchouli roots by 54.8to 70.6% and suppressed nematode population with pf/pi value 0.61 to0.94. Growth of inoculated plants increased by 37.86 to 84.71% comparedwith uninoculated (control) ones.</p><p>Key words: Pogostemon cablin, endophytic bacteria, effectivenessnematode, Pratylenchus brachyurus, biological control</p>

scholarly journals Isolamento e avaliação da atividade nematicida de constituintes químicos de Mucuna cinerea contra Meloidogyne incognita e Heterodera glycines

Química Nova ◽  
2003 ◽  
Vol 26 (3) ◽  
pp. 335-339 ◽  
Author(s):  
Antonio Jacinto Demuner ◽  
Luiz Cláudio de Almeida Barbosa ◽  
Jeferson Chagas do Nascimento ◽  
Jessy James Vieira ◽  
Maria Amelia dos Santos
Keyword(s):  
Meloidogyne Incognita ◽  
Heterodera Glycines

Responses of Heterodera glycines and Meloidogyne incognita to exogenously applied biogenic amines

Nematology ◽  
2008 ◽  
Vol 10 (6) ◽  
pp. 911-917 ◽  
Author(s):  
Edward Masler
Keyword(s):  
Biogenic Amines ◽  
Meloidogyne Incognita ◽  
Head Movement ◽  
Heterodera Glycines ◽  
Parasitic Nematodes ◽  
Stage Juvenile ◽  
Quantitative Analyses ◽  
Effective Doses ◽  
Plant Parasitic

AbstractHatching and head movement behaviours of second-stage juvenile (J2) of two agriculturally important plant-parasitic nematodes were affected by the in vitro application of biogenic amines. The behavioural responses of Heterodera glycines and Meloidogyne incognita to treatments of serotonin, octopamine and dopamine were qualitatively similar, but significant quantitative differences between the species were revealed. The frequency of J2 head movement was decreased by as little as 250 μM serotonin in H. glycines and 500 μM serotonin in M. incognita, with effective doses (ED50) of 0.73 mM for H. glycines and 1.72 mM for M. incognita. Octopamine had the opposite effect of serotonin, increasing J2 head movement frequency at thresholds of 2 mM in H. glycines and 1 mM in M. incognita. Octopamine ED50 values were 32.35 mM and 1.91 mM, respectively. Dopamine had no effect on head movement in either species up to concentrations of 20 mM. Serotonin inhibited hatch in both species but was more potent against H. glycines (90% inhibition at 1 mM) than M. incognita (40% inhibition at 5 mM). Octopamine reduced hatch equally in both species with over 95% inhibition at 80 mM. Dopamine had no effect on hatch in M. incognita but did inhibit H. glycines hatch over 60% at 40 mM. The value of detailed quantitative analyses of plant-parasitic nematode responses to biogenic amines for studies on nematode control is discussed.

Behaviour of Heterodera glycines and Meloidogyne incognita infective juveniles exposed to nematode FMRFamide-like peptides in vitro

Nematology ◽  
2012 ◽  
Vol 14 (5) ◽  
pp. 605-612 ◽  
Author(s):  
Edward P. Masler ◽  
Asmita Nagarkar ◽  
Lanelle Edwards ◽  
Cerruti R.R. Hooks
Keyword(s):  
Amino Acid ◽  
Meloidogyne Incognita ◽  
Head Movement ◽  
Heterodera Glycines ◽  
Amino Acid Sequences ◽  
Native Peptide ◽  
Large Numbers ◽  
Movement Frequency ◽  
Level Analysis

The effects of 1 mM solutions of FMRFamide-like peptides (FLPs) on the behaviours of Heterodera glycines and Meloidogyne incognita infective juveniles (J2) were examined in vitro. Seven FLPs, representing products of five flp genes and comprising a variety of amino acid sequences, were tested for their effects on J2 head movement frequency. Distinct differences in species responses were observed. KHEYLRFa and KSAYMRFa caused increased head movement frequencies in each species. In H. glycines KHEYLRFa was 2.9-fold more potent than KSAYMRFa. In M. incognita the potencies were equal. KHEYLRFa and KSAYMRFa each stimulated H. glycines J2 more effectively than M. incognita J2. However, two additional FLPs, AQTFVRFa and SAPYDPNFLRFa, were stimulatory in M. incognita but not in H. glycines. KPNFIRFa, KPNFLRFa and RNSSPLGTMRFa had no effect on either species. Substitution of d-amino acids at any position in KHEYLRFa resulted in decreased stimulation of head movement relative to the native peptide in each species, but all of the d-amino acid KHEYLRFa analogues were stimulatory relative to untreated controls. d-amino acid substitutions in KSAYMRFa eliminated stimulatory activity in M. incognita by all analogues except dKSAYMRFa. In H. glycines, only KSdAYMRFa and KSAYMdRFa were not stimulatory, and KSAYdMRFa stimulated equal to the native peptide. The remaining four analogues each stimulated relative to controls but below the native peptide level. Analysis of the head movement behaviour of large numbers of J2 of each species demonstrated that behaviours are quite stable and responses to FLP treatment are highly predictable.

scholarly journals BIOLOGICAL NEMATICIDES ASSOCIATED WITH POULTRY LITTER IN THE CONTROL OF NEMATODES IN SOYBEAN AND SECOND CORN CROP

2021 ◽  
Vol 34 (3) ◽  
pp. 580-589
Author(s):  
TAYRLEN EDUARDO AMORIM ROSA ◽  
JEAN CRAMENAK DE SOUZA ◽  
WELLINGTON JOSÉ PEREIRA ◽  
JANAINA ALVES DE ALMEIDA MOREIRA ◽  
FERNANDO GODINHO DE ARAÚJO
Keyword(s):  
Heterodera Glycines ◽  
Block Design ◽  
Poultry Litter ◽  
Growing Season ◽  
Fresh Weight ◽  
Management Measures ◽  
Randomized Block Design ◽  
Pratylenchus Brachyurus ◽  
Infested Field ◽  
Soybean Plants

ABSTRACT Alternative management measures have been used to reduce nematode population levels in affected areas. In this perspective, the objective was to evaluate the efficiency of nematicides based on fungus and bacterium, associated with poultry litter applied via ground in the management of Pratylenchus brachyurus and Heterodera glycines in soybean and corn crops in succession. The experiment was conducted in the 2018/19 growing season in the municipality of Ipameri– GO (-17°34’55.58”S−48°12’02.35”O) in naturally infested field, arranged in a randomized block design, with 10 treatments and 4 replicates, being: T1- Witness/ control, T2- Poultry litter, T3- P. lilacinum + T. harzianum, T4- B. subtilis + B. licheniformis, T5- P. chlamydosporia, T6- Abamectina, T7- P. lilacinum + T. harzianum + Poultry litter, T8- B. subtilis + B. licheniformis + Poultry litter, T9- P. chlamydosporia + Poultry litter, T10- Abamectina + Poultry litter. The poultry litter was added in the soil 10 days before planting. The plots were composed of 6 lines of 6 m, with a spacing of 0.5 m. In the soybean crop at 45 and 90 days after sowing (DAS), the fresh weight of the roots (MFR), nematological variables and productivity were evaluated. In corn, only nematological variables were evaluated at 60 DAS. The treatments using P. lilacinum + T. harzianum and B. subtillis + B. licheniformes increased the MFR and reduced the number of P. brachyurus and H. glycines at the root. P. chlamydosporia associated with poultry litter increased in productivity in soybean plants.

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