scholarly journals Effects of Total Replacement of Atrial Myosin Light Chain-2 With the Ventricular Isoform in Atrial Myocytes of Transgenic Mice

Circulation ◽  
1998 ◽  
Vol 97 (15) ◽  
pp. 1508-1513 ◽  
Author(s):  
Corinn M. Pawloski-Dahm ◽  
Guojie Song ◽  
Darryl L. Kirkpatrick ◽  
Joe Palermo ◽  
James Gulick ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Atrial Myocytes ◽  
Total Replacement ◽  
Myosin Light Chain 2 ◽  
Atrial Myosin

scholarly journals Chamber specification of atrial myosin light chain-2 expression precedes septation during murine cardiogenesis

1994 ◽  
Vol 269 (24) ◽  
pp. 16961-16970
Author(s):  
S.W. Kubalak ◽  
W.C. Miller-Hance ◽  
T.X. O'Brien ◽  
E. Dyson ◽  
K.R. Chien
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Myosin Light Chain 2 ◽  
Atrial Myosin

scholarly journals Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene.

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009 ◽  
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

scholarly journals Overexpression of heart-specific small subunit of myosin light chain phosphatase results in heart failure and conduction disturbance

2018 ◽  
Vol 314 (6) ◽  
pp. H1192-H1202 ◽  
Author(s):  
Takuro Arimura ◽  
Antoine Muchir ◽  
Masayoshi Kuwahara ◽  
Sachio Morimoto ◽  
Taisuke Ishikawa ◽  
...  
Keyword(s):  
Heart Failure ◽  
Transgenic Mice ◽  
Muscle Contraction ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Small Subunit ◽  
Conduction Disturbance ◽  
Myosin Light Chain Phosphatase ◽  
Myosin Light Chain 2

Mutations in genes encoding components of the sarcomere cause cardiomyopathy, which is often associated with abnormal Ca2+ sensitivity of muscle contraction. We have previously shown that a heart-specific myosin light chain phosphatase small subunit (hHS-M21) increases the Ca2+ sensitivity of muscle contraction. The aim of the present study was to investigate the function of hHS-M21 in vivo and the causative role of abnormal Ca2+ sensitivity in cardiomyopathy. We generated transgenic mice with cardiac-specific overexpression of hHS-M21. We confirmed that hHS-M21 increased the Ca2+ sensitivity of cardiac muscle contraction in vivo, which was not followed by an increased phosphorylation of myosin light chain 2 isoforms. hHS-M21 transgenic mice developed severe systolic dysfunction with myocardial fibrosis and degeneration of cardiomyocytes in association with sinus bradycardia and atrioventricular conduction defect. The contractile dysfunction and cardiac fibrosis were improved by treatment with the Rho kinase inhibitor fasudil. Our findings suggested that the overexpression of hHS-M21 results in cardiac dysfunction and conduction disturbance via non-myosin light chain 2 phosphorylation-dependent regulation. NEW & NOTEWORTHY The present study is the first to develop mice with transgenic overexpression of a heart-specific myosin light chain phosphatase small subunit (hHS-M21) and to examine the effects of hHS-M21 on cardiac function. Elevation of hHS-M21 induced heart failure with myocardial fibrosis and degeneration of cardiomyocytes accompanied by supraventricular arrhythmias.

The murine atrial myosin light chain-2 gene:a member of an evolutionarily conserved family of contractile proteins

2000 ◽  
Vol 90 (3-4) ◽  
pp. 248-252 ◽  
Author(s):  
P.A. Doevendans ◽  
R. Bronsaer ◽  
P.R. Lozano ◽  
S. Kubalak ◽  
M. van Bilsen
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Contractile Proteins ◽  
Myosin Light Chain 2 ◽  
Evolutionarily Conserved ◽  
Atrial Myosin

Expression of the rat myosin light-chain 2 gene in transgenic mice: stage specificity, developmental regulation, and interrelation with the endogenous gene

1988 ◽  
Vol 8 (2) ◽  
pp. 1006-1009
Author(s):  
M Shani ◽  
I Dekel ◽  
O Yoffe
Keyword(s):  
Transgenic Mice ◽  
Light Chain ◽  
Myosin Light Chain ◽  
Developmental Regulation ◽  
Endogenous Gene ◽  
Temporal Regulation ◽  
Stage Specificity ◽  
Myosin Light Chain 2 ◽  
Skeletal Myosin ◽  
Transgenic Strains

The expression of the rat skeletal myosin light-chain 2 gene in two transgenic strains was tissue specific and stage specific. However, the temporal regulation during development of the transgene was different from that of the endogenous gene. Surprisingly, in one strain, the expression of the transgene was associated with a significant down-regulation of the endogenous gene. The possible mechanisms to account for the suppression of the endogenous gene and the potential implications of this suppression are discussed.

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