Effects of a Long-Term Treatment With Aliskiren or Ramipril on Structural Alterations of Subcutaneous Small-Resistance Arteries of Diabetic Hypertensive Patients

Hypertension ◽  
2014 ◽  
Vol 64 (4) ◽  
pp. 717-724 ◽  
Author(s):  
Carolina De Ciuceis ◽  
Carmine Savoia ◽  
Emanuele Arrabito ◽  
Enzo Porteri ◽  
Monica Mazza ◽  
...  
Keyword(s):  
Term Treatment ◽  
Resistance Arteries ◽  
Hypertensive Patients ◽  
Structural Alterations ◽  
Long Term Treatment ◽  
Small Resistance

Diuretics ??? a risk in the long-term treatment of hypertensive patients?

1988 ◽  
Vol 6 (11) ◽  
pp. 925-930 ◽  
Author(s):  
Antoon Amery ◽  
Willem Birkenh??ger ◽  
Christopher Bulpitt ◽  
Denis Clement ◽  
Peter de Leeuw ◽  
...  
Keyword(s):  
Term Treatment ◽  
Hypertensive Patients ◽  
Long Term Treatment

Intact endothelial and smooth muscle function in small resistance arteries after 48 h in vessel culture

2000 ◽  
Vol 279 (3) ◽  
pp. H1434-H1439 ◽  
Author(s):  
Steffen-Sebastian Bolz ◽  
Susanne Pieperhoff ◽  
Cor De Wit ◽  
Ulrich Pohl
Keyword(s):  
Smooth Muscle ◽  
Vascular Function ◽  
Calf Serum ◽  
Term Treatment ◽  
Resistance Arteries ◽  
Response Curves ◽  
Vascular Integrity ◽  
Long Term Treatment ◽  
And Function

Long-term culture of resistance vessels allows introduction of molecular biology techniques for use in microvascular research. The aim of the present study was to establish a culture protocol that preserved vascular integrity and function in microvessels for 48 h in culture. Skeletal muscle resistance arteries were excised from the hamster gracilis muscle. Segments were assigned to immediate functional tests or to vessel culture, during which segments were perfused and superfused at a transmural pressure of 45 mmHg with Leibovitz (L15) medium containing 15% fetal calf serum and antibiotics for 48 h. Cultured and freshly isolated vessels showed similar levels of spontaneous tone, myogenic responses, changes in smooth muscle intracellular calcium (Cai 2+) (fura 2), and vascular diameter (video microscopy) in response to 0.3 M norepinephrine and similar concentration-response curves for acetylcholine (endothelium dependent, ± N ω-nitro-l-arginine) and sodium nitroprusside (endothelium independent). Measurements of endothelial Cai 2+ revealed similar acetylcholine-induced increases in endothelial Cai 2+ in both groups. It is concluded that vascular function can be preserved while maintaining vessels in culture. Thus it is possible to utilize protocols that require long-term treatment.

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