Intact endothelial and smooth muscle function in small resistance arteries after 48 h in vessel culture

Long-term culture of resistance vessels allows introduction of molecular biology techniques for use in microvascular research. The aim of the present study was to establish a culture protocol that preserved vascular integrity and function in microvessels for 48 h in culture. Skeletal muscle resistance arteries were excised from the hamster gracilis muscle. Segments were assigned to immediate functional tests or to vessel culture, during which segments were perfused and superfused at a transmural pressure of 45 mmHg with Leibovitz (L15) medium containing 15% fetal calf serum and antibiotics for 48 h. Cultured and freshly isolated vessels showed similar levels of spontaneous tone, myogenic responses, changes in smooth muscle intracellular calcium (Cai 2+) (fura 2), and vascular diameter (video microscopy) in response to 0.3 M norepinephrine and similar concentration-response curves for acetylcholine (endothelium dependent, ± N ω-nitro-l-arginine) and sodium nitroprusside (endothelium independent). Measurements of endothelial Cai 2+ revealed similar acetylcholine-induced increases in endothelial Cai 2+ in both groups. It is concluded that vascular function can be preserved while maintaining vessels in culture. Thus it is possible to utilize protocols that require long-term treatment.

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Effects of a Long-Term Treatment with Alacepril on Left Ventricular Hypertrophy and Function in Patients with Essential Hypertension

The Journal of Clinical Pharmacology ◽

10.1002/j.1552-4604.1992.tb05779.x ◽

1992 ◽

Vol 32 (7) ◽

pp. 667-670 ◽

Cited By ~ 2

Author(s):

Takumi Sumimoto ◽

Takaaki Ochi ◽

Kunio Hiwada

Keyword(s):

Essential Hypertension ◽

Left Ventricular Hypertrophy ◽

Ventricular Hypertrophy ◽

Term Treatment ◽

Left Ventricular ◽

Long Term Treatment ◽

And Function

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Long term treatment of neonatal aortic smooth muscle cells with βVLDL induces cholesterol accumulation

Atherosclerosis ◽

10.1016/0021-9150(92)90023-a ◽

1992 ◽

Vol 95 (2-3) ◽

pp. 201-210 ◽

Cited By ~ 10

Author(s):

Barbara M. Schreiber ◽

H. Vernon Jones ◽

Paul Toselli ◽

Carl Franzblau

Keyword(s):

Smooth Muscle ◽

Smooth Muscle Cells ◽

Muscle Cells ◽

Term Treatment ◽

Cholesterol Accumulation ◽

Aortic Smooth Muscle Cells ◽

Aortic Smooth Muscle ◽

Long Term Treatment

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Biocompatibility of Charcoal Hemoperfusion. Effects of Long-Term Treatment on Lymphocyte Characteristics and Function

The International Journal of Artificial Organs ◽

10.1177/039139888600900507 ◽

1986 ◽

Vol 9 (5) ◽

pp. 301-304 ◽

Cited By ~ 3

Author(s):

S. Stefoni ◽

A. Nanni Costa ◽

G. Liviano D'Arcangelo ◽

M. Biavati ◽

S. lannelli ◽

...

Keyword(s):

Antigen Expression ◽

Term Treatment ◽

T Cell Subsets ◽

Long Term Treatment ◽

Circulating Lymphocytes ◽

And Function ◽

Charcoal Hemoperfusion

Biocompatibility of charcoal hemoperfusion was studied in a group of 15 uremic patients, evaluating the effects of long-term treatment on some structural and functional parameters of circulating lymphocytes: in vivo distribution of T-cell subsets; surface T3, T4 and T8 antigen expression, in vivo and in vitro DNA synthesis. A comparative analysis was performed with patients on conventional dialysis using cuprophan membranes.

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Comparative evaluation of methods testing in vitro sensitivity to azithromycin in multi-drug resistant Pseudomonas aeruginosa isolated from cystic fibrosis patients

10.21203/rs.3.rs-46745/v1 ◽

2020 ◽

Author(s):

Michael Soerensen ◽

Dennis Nurjadi ◽

Bakhodur Khakimov ◽

Sébastien Boutin ◽

Alexander H. Dalpke ◽

...

Keyword(s):

Cystic Fibrosis ◽

Therapeutic Option ◽

Calf Serum ◽

Multidrug Resistant ◽

Term Treatment ◽

In Vitro Sensitivity ◽

Mueller Hinton ◽

Long Term Treatment

Abstract Long-term treatment with azithromycin is a therapeutic option in Cystic Fibrosis (CF) patients chronically infected with P. aeruginosa . It was recently shown that azithromycin has direct antimicrobial activity when P. aeruginosa isolates are tested in Roswell Park Memorial Institute medium supplemented with fetal calf serum (RPMI 1640/FCS) by broth microdilution. We now investigated whether (i) azithromycin might also be active against multidrug resistant (MDR) P. aeruginosa isolated from CF patients and (ii) how in vitro sensitivity assays perform in synthetic cystic fibrosis sputum medium (SCFM), a medium that mimics the particular CF airway environment. In 17 (59%) out of 29 MDR P. aeruginosa CF isolates MICs for azithromycin ranged between 0.25 and 8 µg/ml and 12 isolates (41%) showed a MIC ≥512 µg/ml when measured in RPMI/FCS. In contrast, MICs were ≥256 µg/ml for all P. aeruginosa MDR isolates when tested in either SCFM or in conventional cation-adjusted Mueller Hinton Broth. High MIC values observed in CF adapted medium SCFM for both PAO1 and MDR P. aeruginosa CF isolates, as opposed to findings in RPMI, argue against routine azithromycin MIC testing of CF isolates.

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Increase in circulating CD4+CD25+Foxp3+ T cells in patients with Philadelphia-negative chronic myeloproliferative neoplasms during treatment with IFN-α

Blood ◽

10.1182/blood-2011-03-340992 ◽

2011 ◽

Vol 118 (8) ◽

pp. 2170-2173 ◽

Cited By ~ 49

Author(s):

Caroline Hasselbalch Riley ◽

Morten Krogh Jensen ◽

Marie Klinge Brimnes ◽

Hans Carl Hasselbalch ◽

Ole Weis Bjerrum ◽

...

Keyword(s):

T Cells ◽

Regulatory T Cells ◽

Myeloproliferative Neoplasms ◽

Term Treatment ◽

Immune Reactivity ◽

Chronic Myeloproliferative Neoplasms ◽

Healthy Donors ◽

Long Term Treatment ◽

And Function

Abstract Recent reports have described complete or major molecular remission in patients with polycythemia vera after long-term treatment with the immunomodulatory agent IFN-α2. Accordingly, there are reasons to believe that the immune system is a key player in eradicating the JAK2 mutated clone in these patients. Foxp3+ regulatory T cells play a pivotal role in maintaining immune homeostasis and, importantly, preventing immune reactivity to self-antigens; however, their suppressive activity can compromise an effective antitumor immune response, and high frequencies of regulatory T cells in peripheral blood have been reported in both hematologic and solid cancers. We have analyzed the number, phenotype, and function of circulating CD4+CD25+Foxp3+ T cells in patients with chronic myeloproliferative neoplasms. Surprisingly, we found a marked expansion of this subset of lymphocytes in patients treated with IFN-α2 (13.0%; 95% confidence interval [CI] 10.8% to 15.2%) compared with healthy donors (6.1%; 95% CI 4.9% to 7.2%), patients with untreated chronic myeloproliferative neoplasms (6.9%; 95% CI 5.8% to 7.4%), or patients treated with hydroxyurea (5.8%; 95% CI 4.3% to 7.4%; P < .0001).

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