A new entomopathogenic nematode, Steinernema colombiense n. sp. (Nematoda: Steinernematidae), from Colombia

Nematology ◽  
2008 ◽  
Vol 10 (4) ◽  
pp. 561-574 ◽  
Author(s):  
Juan Carlos López-Núñez ◽  
Kathryn Plichta ◽  
Carmenza E. Góngora-Botero ◽  
S. Patricia Stock
Keyword(s):  
Excretory Pore ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Infective Juvenile ◽  
Diagnostic Features ◽  
Rdna Sequences ◽  
Rdna Sequence Data

Abstract A new entomopathogenic nematode, Steinernema colombiense n. sp., is described from Colombia. Morphological, molecular (28S and ITS rDNA sequence data) and cross-hybridisation studies were used for diagnostics and identification purposes. In addition, 28S and ITS rDNA sequence data were used to assess evolutionary relationships of the new species with other Steinernema spp. Morphological diagnostic features for S. colombiense n. sp. include morphometric features of the third-stage infective juvenile, including body length of 636 (549-732) μm, narrow body diam. (31 (22-36) μm), position of the excretory pore (35 (31-40) μm), tail length (41 (32-53) μm), D% = 29 (25-33) and E% = 205 (138-284). In addition, males of first and second generations are characterised by the morphology of the spicules and gubernaculum, the number and arrangement of the genital papillae and the excretory pore position (at 67 (56-76) and 54 (46-63) μm, for first and second generations, respectively). In addition to these traits, 28S and ITS rDNA sequences analyses both showed this species to be a distinct and unique entity.

Steinernema ashiuense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Japan

Nematology ◽  
2006 ◽  
Vol 8 (5) ◽  
pp. 681-690 ◽  
Author(s):  
Long K. Phan ◽  
Shuhei Takemoto ◽  
Kazuyoshi Futai
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Morphological Characters ◽  
Rdna Sequence ◽  
Infective Juvenile ◽  
Lateral Field ◽  
Spicule Length

Abstract Steinernema ashiuense sp. n. was collected by a Galleria baiting method from grassland along the riverbank of the Yura river, near the Ashiu Research Forest Station of Kyoto University. The new species is characterised by an infective juvenile body length of 768 (720-800) μm, lateral field with five equally developed ridges (i.e., six lines or incisures), head smooth lacking horn-like structures, excretory pore located at level of middle of pharynx, hyaline portion short (ca one-third of tail length). Males have 50-65 μm long, broad, slightly yellowish spicules; spicule length / spicule width = 4 (3.5-4.4), and two subventral and one subdorsal pair of genital papillae in the postcloacal region. The analysis of ITS-rDNA sequence placed S. ashiuense sp. n. in the 'feltiae-kraussei-oregonense' group in the clade containing S. robustispiculum and S. monticolum. It can be distinguished from these species by morphological characters of the infective juveniles and males.

Molecular phylogeny and status of Diploicia and Diplotomma, with observations on Diploicia subcanescens and Diplotomma rivas-martinezii

2002 ◽  
Vol 34 (6) ◽  
pp. 509-519 ◽  
Author(s):  
María del Carmen Molina ◽  
Ana Crespo ◽  
Oscar Blanco ◽  
Néstor Hladun ◽  
David L. Hawksworth
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Molecular Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
New Combination ◽  
Success Rates ◽  
Species Pair ◽  
Monophyletic Clade ◽  
Rdna Sequence Data

AbstractITS rDNA sequence data shows that Diploicia and Diplotomma species form a monophyletic clade distinct from other Buellia species. This indicates that Diplotomma merits acceptance as a genus, and suggests that Diploicia should be treated as a synonym of Diplotomma, the earlier name. The data also shows Diploicia subcanescens, considered the fertile counterpart in a species pair with D. canescens, is nested within D. canescens and should be treated as a synonym despite reported chemical differences. In addition, the molecular data support the distinctness of Diplotomma rivas-martinezii, a species restricted to gypsum rocks in Spain, from the widespread D. venustum, which grows on calcareous rocks. Aposymbiotic cultures suggest that D. rivas-martinezii also differs from D. venustum in its germination and isolation success rates. One new combination is made: Diplotomnta pulverulenta (Anzi) D. Hawksw. (syn. Abrothallus pulverulentus Anzi) for the lichenicolous species previously known as Buellia pulverulenta.

Heterorhabditis gerrardi n. sp. (Nematoda: Heterorhabditidae): the hidden host of Photorhabdus asymbiotica (Enterobacteriaceae: γ-Proteobacteria)

2009 ◽  
Vol 83 (4) ◽  
pp. 309-320 ◽  
Author(s):  
K.L. Plichta ◽  
S.A. Joyce ◽  
D. Clarke ◽  
N. Waterfield ◽  
S.P. Stock
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Entomopathogenic Nematode ◽  
Tail Length ◽  
Its Region ◽  
Nematode Species ◽  
Diagnostic Features ◽  
Cross Hybridization ◽  
Group V ◽  
Terminal Set

AbstractA new entomopathogenic nematode species from Australia, Heterorhabditis gerrardi n. sp. (Nematoda: Heterorhabditidae) is described. Morphological and molecular studies together with cross-hybridization tests indicated that this nematode represents a new undescribed species, closely related to members in the ‘indica-group’. However, the new species can be distinguished from other species in this genus by a combination of several qualitative and quantitative morphological traits. Key diagnostic features include: body size and excretory pore position of the third-stage infective juveniles; male bursa with a reduction of bursal rays, usually affecting the terminal set of papillae, with symmetrical or asymmetrical loss of one or two pairs; vulva of hermaphrodites more anteriorly located than in other species in the indica-group (V% average: 43), with non-protruding or slightly protruding lips, and longer tail length (average: 106 μm). The new species can be further characterized by molecular traits of sequence data from the internal transcribed spacer (ITS) region of ribosomal DNA. Additionally, the bacterial symbiont of this new species, Photorhabdus asymbiotica Kingscliff strain, was phenotypically characterized and compared with other P. asymbiotica strains. The Kingscliff strain revealed many characters not present in other strains of this species. We hypothesize that the newly found traits may contribute to the maintenance of this mutualistic association of the bacterium with its nematode host.

Congruence of morphological, reproductive and ITS rDNA sequence data in some AustralasianEunotia bilunaris(Bacillariophyta)

2007 ◽  
Vol 42 (1) ◽  
pp. 61-79 ◽  
Author(s):  
Pieter Vanormelingen ◽  
Victor A. Chepurnov ◽  
David G. Mann ◽  
Sylvie Cousin ◽  
Wim Vyverman
Keyword(s):  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data

Genetic variation amongSclerotiumisolates from Benin and South Africa, determined using mycelial compatibility and ITS rDNA sequence data

2005 ◽  
Vol 34 (1) ◽  
pp. 19 ◽  
Author(s):  
Appolinaire Adandonon ◽  
Theresa A. S. Aveling ◽  
Nicolaas A. van der Merwe ◽  
Gina Sanders
Keyword(s):  
South Africa ◽  
Genetic Variation ◽  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data ◽  
Mycelial Compatibility

A reassessment of excavated Tuber species from China based on morphology and ITS rDNA sequence data

Mycotaxon ◽  
2013 ◽  
Vol 124 (1) ◽  
pp. 155-163 ◽  
Author(s):  
Li Fan ◽  
Jin-Zhong Cao ◽  
Yu Li
Keyword(s):  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Rdna Sequence Data

Two new Terfezia species from Southern Europe

Phytotaxa ◽  
2015 ◽  
Vol 230 (3) ◽  
pp. 239 ◽  
Author(s):  
Juan Julián Bordallo ◽  
Antonio Rodríguez ◽  
Vasileios Kounas ◽  
Francisco Camello ◽  
Mario Honrubia ◽  
...  
Keyword(s):  
New Species ◽  
Host Plants ◽  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
Similar Species ◽  
Southern Europe ◽  
Phylogenetic Data ◽  
Rdna Sequence Data ◽  
Two New Species

Two new species of Terfezia, Terfezia grisea and Terfezia cistophila, are documented from Spain and Greece, based on morphology and ITS-rDNA sequence data. Macro and micro descriptions with illustrations and ITS phylogenetic data for the two species are provided, which are discussed in relation to similar species in this genus and their host plants.

A new species of Podosphaera sect. Sphaerotheca subsect. Sphaerotheca from India—first report of powdery mildew causing wilting and ultimately death of leaves of Filipendula vestita

Phytotaxa ◽  
2021 ◽  
Vol 491 (2) ◽  
pp. 131-142
Author(s):  
SANJAY YADAV ◽  
SANJEET KUMAR VERMA ◽  
RAGHVENDRA SINGH
Keyword(s):  
New Species ◽  
Powdery Mildew ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Rdna Sequence ◽  
Its Rdna ◽  
First Report ◽  
Rdna Sequence Data ◽  
A New Species

Podosphaera filipendulensis, a new teleomorph of powdery mildew was discovered on living leaves of Filipendula vestita (Rosaceae). It is the first species of powdery mildew that causes wilting and ultimately death of apical infected leaves of F. vestita. It is described, illustrated and compared with other Podosphaera species reported on Rosaceae and a key to all species reported on Rosaceae is provided. The phylogeny of this species has been inferred from internal transcribed spacer (ITS) rDNA sequence data. Phylogenetically, P. filipendulensis is close to P. ferruginea var. ferruginea and P. macularis but differs in having larger chasmothecia, smaller peridium cells, shorter, narrower and aseptate appendages, 8-spored asci and smaller ascospores.

Heterorhabditis atacamensis n. sp. (Nematoda: Heterorhabditidae), a new entomopathogenic nematode from the Atacama Desert, Chile

2010 ◽  
Vol 85 (4) ◽  
pp. 381-394 ◽  
Author(s):  
S. Edgington ◽  
A.G. Buddie ◽  
D. Moore ◽  
A. France ◽  
L. Merino ◽  
...  
Keyword(s):  
Excretory Pore ◽  
Entomopathogenic Nematode ◽  
Atacama Desert ◽  
Molecular Data ◽  
Complete Sequence ◽  
Valid Species ◽  
Infective Juvenile ◽  
Adult Males ◽  
Rdna Sequences ◽  
Body Diameter

AbstractA new Heterorhabditis species of entomopathogenic nematode was isolated from soil of the Atacama Desert in Chile. The new species is characterized by morphometrics of the infective juvenile (IJ) with length (L) = 611 (578–666) μm, head to excretory pore length (EP) = 115 (101–126) μm, tail = 69 (62–79) μm long, (EP/tail) × 100 (E%) = 165 (149–182) and L/maximum body diameter (ratio a) = 28 (25–31). The male has spicules 45 (40–49) μm long, gubernaculum 20 (17–22) μm long and (spicule length/anal body diameter) × 100 (SW%) = 205 (179–249). The hermaphroditic adult has shallow cuticular folds immediately anterior and posterior to the vulva, a slight post-anal swelling and a finely rounded tail terminus. Morphologically, H. atacamensis n. sp. resembles H. safricana, H. marelatus, H. downesi and H. amazonensis, but can be distinguished by characters of adult and IJ stages. In particular, for adult males, H. atacamensis n. sp. differs from H. amazonensis by the number and orientation of the genital papillae and from H. downesi by the position of the excretory pore; by the shape of the female tail terminus from H. downesi and by the position of the IJ hemizonid from H. marelatus. Heterorhabditis atacamensis n. sp. is further characterized by internal transcribed spacer (ITS) and D2D3 rDNA sequences, the closest species, H. safricana, being separated by 13 bp across 730 bp of the ITS (incorporating ITS1 (partial sequence), 5.8S (complete sequence), ITS2 (complete sequence)) and 5 bp across 592 bp of the partial 28S (incorporating D2D3) sequence. The morphological and molecular data confirm that H. atacamensis n. sp. is a valid species.

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