Role of Loop Mediated Isothermal Amplification Assay in Detecting Mycobacterium Tuberculosis

2019 ◽  
Author(s):  
K. Jana ◽  
K.V.V.V. Kaminedi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Assay

Diagnosis of osteoarticular tuberculosis: multi-targeted loop-mediated isothermal amplification assay versus multiplex-PCR

2021 ◽  
Author(s):  
Anish Khan ◽  
Ekta Kamra ◽  
Raj Singh ◽  
Vikrant Sharma ◽  
Vishwajeet Singh ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Lamp Assay ◽  
Cost Effective ◽  
Test Methods ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Osteoarticular Tuberculosis ◽  
Cost Effective Method ◽  
Amplification Assay

Aim: Diagnosis of osteoarticular tuberculosis (OATB) is quite challenging and there is an urgent need to design a prompt and precise diagnostic test. Methods: We developed a multi-targeted loop-mediated isothermal amplification (LAMP) assay using mpt64 (Rv1980c) and pstS1 (Rv0934) targets for the detection of Mycobacterium tuberculosis in OATB patients. Results: The sensitivities of 100 and 82.4% were obtained in confirmed (n = 10) and suspected (n = 57) OATB cases, respectively by multi-targeted LAMP with a specificity of 96.9% (n = 33). Moreover, the sensitivities attained by multi-targeted LAMP in total OATB cases were significantly higher (p < 0.05–0.01) than multiplex-PCR ( mpt64 +  pstS1) and GeneXpert assay. Conclusion: Our LAMP is simple, reliable and cost-effective method, which may develop into an attractive diagnostic kit for early detection of OATB cases.

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