Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content

Phytotaxa ◽  
2016 ◽  
Vol 265 (3) ◽  
pp. 238 ◽  
Author(s):  
MINE KOÇYIĞIT ◽  
METIN TUNA
Keyword(s):  
Content Analysis ◽  
Cross Sections ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Taxonomic Revision ◽  
Chromosome Length ◽  
Data Matrix ◽  
Vascular Bundles ◽  
Taxonomic Markers

Anatomy of leaf cross sections, karyological studies, and nuclear DNA content analysis by flow cytometry were carried out on the six Sternbergia species from Turkey: S. candida (endemic to the country), S. clusiana, S. colchiciflora, S. lutea, S. sicula and S. vernalis. In karyological analysis, number and morphology of the somatic chromosomes, the ploidy level and karyotype formula of the specimens used in the study were investigated in detail. The measurements of somatic chromosomes were calculated with formula of the relative variation in chromosome lenght (CVCL) and mean centromeric asymmetry (MCA). According to the results of this study, there are two different basic chromosome numbers. i.e. x = 10 (S. candida, S. clusiana, S. colchiciflora and S. vernalis) and x = 11 (S. lutea and S. sicula). Results of karyotype analysis corroborated results of previous studies. The smallest chromosome length was measured as 3.05 μm in S. clusiana while the longest length of 20.89 μm was observed in S. sicula. This is the first study reporting nuclear DNA content results for Sternbergia. Based on results of the nuclear DNA content analysis, there is a great variation within the genus in the interspecific level as 2C DNA content varies between 54.03 and 80.42 pg among the species investigated in the study. Anatomical features of the leaves were good taxonomic markers, especially concerning their palisade cells, ribs, cuticle, laticifer canals, vascular bundles, crystals, stomatal index, and stomata structure. Analytical drawings were presented. Six noteworthy leaf characters were quantified, organized in a data matrix, and analysed with UPGMA methods. In conclusion, cytological characteristics of the Sternbergia species together with their leaf features proved to be quite useful in identification and therefore can provide more clarity for taxonomic revision of the genus.

scholarly journals Cytogenetic and Nuclear DNA Content Analysis in Anostomidae Fishes from the Sapucai River, Minas Gerais State, Brazil.

CYTOLOGIA ◽  
2002 ◽  
Vol 67 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Mirna Aparecida Pereira ◽  
Claudio Oliveira ◽  
Fausto Foresti ◽  
Edson Luis Maistro
Keyword(s):  
Content Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Minas Gerais ◽  
Minas Gerais State

scholarly journals Equisetum ×moorei Newman (Equisetaceae) – a ‘new’ nothotaxon in the Polish flora

2016 ◽  
Vol 41 (1) ◽  
pp. 11-18 ◽  
Author(s):  
Paweł Kalinowski ◽  
Elwira Sliwinska ◽  
Jerzy Kruk
Keyword(s):  
Content Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Field Studies ◽  
Habitat Requirements ◽  
Triploid Hybrid ◽  
Historical Distribution ◽  
Diploid Taxon ◽  
S Poland

Abstract To investigate present and historical distribution of Equisetum ×moorei in Poland and its habitat requirements, field studies at sites of potential occurrence of this hybrid taxon as well as literature and herbarium search were performed. As a results of these investigations, E. ×moorei was found at five contemporary and at a few historical localities in the present territory of Poland. Since the Equisetum populations near Olkusz (S Poland) showed phenotype similar, to some extent, to triploid hybrid E. ×ascendens, we performed nuclear DNA content analysis of these populations. However, it turned out that the investigated individuals belonged to a diploid taxon that can be ascribed to E. ×moorei.

scholarly journals Cytogenetic Characterization and Nuclear DNA Content of Diploid and Tetraploid Forms of Stokes Aster

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2005-2012
Author(s):  
Jessica Gaus Barb ◽  
Dennis J. Werner ◽  
Shyamalrau P. Tallury
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome Length ◽  
Slight Reduction ◽  
Meiotic Pairing ◽  
Meiotic Irregularities ◽  
Cytogenetic Characterization ◽  
2C Dna Content ◽  
Chromosome Bridges

Stokesia laevis (J. Hill) Greene is a herbaceous perennial native to the southeastern United States. Most cultivars of Stokesia are diploid (2n = 2x = 14) except for ‘Omega Skyrocket’, a tetraploid (2n = 4x = 28) form selected from a natural population. A comparative study of the karyotypes and meiotic behavior of diploid cultivars, seed-derived accessions of ‘Omega Skyrocket’, synthetically derived autotetraploids, and triploid progeny from these taxa strongly suggest that ‘Omega Skyrocket’ is an autotetraploid form of Stokesia. Total karyotype length, 161 μm and 293 μm, and average chromosome length, 11.5 μm and 10.5 μm, of the diploid cultivars and tetraploid accessions of ‘Omega Skyrocket’, respectively, were determined. The karyotype of the diploid cultivars consisted of eight metacentric (m) and six submetacentric (sm) chromosomes with average arm ratio values ranging from 1.12 to 2.06. The karyotype of ‘Omega Skyrocket’ consisted of 23 m chromosomes and 5 sm chromosomes with average arm ratio values ranging from 1.22 to 2.02. Meiotic pairing in the diploids was normal. No meiotic irregularities such as laggards or bridges were observed and disjunction was balanced (7:7). Accessions of ‘Omega Skyrocket’ demonstrated a high frequency (60%) of quadrivalent formation; however, later stages of meiosis were regular with balanced disjunction (14:14) occurring in 95% of the cells. Meiotic configurations in synthetically derived autotetraploids and triploid hybrids from crosses of diploid cultivars × ‘Omega Skyrocket’ consisted of univalents, bivalents, trivalents, quadrivalents, and pentavalents. Abnormalities, including laggards, unequal and/or premature disjunction, chromosome bridges, and chromosome stickiness were observed. Average nuclear 2C DNA content was 20.3 pg for the diploid cultivars and 39.9 pg for the newly synthesized autotetraploids. Average nuclear 2C DNA content for ‘Omega Skyrocket’ was 37.3 pg, which was 8.2% less than twice the average 2C DNA content of the diploid accessions and 6.4% less than the newly synthesized autotetraploids, suggesting that genomic downsizing in ‘Omega Skyrocket’ has occurred. Similarity of the karyotypes of the diploids and ‘Omega Skyrocket’ and the slight reduction in nuclear DNA content suggest that ‘Omega Skyrocket’ has diverged little from its original diploid progenitor.

Significance of nuclear DNA content analysis in advanced lung cancer using transbronchial biopsy samples.

1993 ◽  
Vol 32 (6) ◽  
pp. 840-845 ◽  
Author(s):  
Akihiko GEMMA ◽  
Syouji HISAKATU ◽  
Yoshimitu YAMANO ◽  
Kouzou YOSHIMORI ◽  
Akira MURATA ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Content Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Transbronchial Biopsy ◽  
Advanced Lung Cancer

scholarly journals A case of basal cell adenocarcinoma undergoing nuclear DNA content analysis.

1998 ◽  
Vol 44 (12) ◽  
pp. 981-983
Author(s):  
Akiyuki MAEDA ◽  
Tsunehisa SHIMODA ◽  
Toshitaka UJI ◽  
Yosihide FUJIMURA ◽  
Junkichi TAKAKI ◽  
...  
Keyword(s):  
Content Analysis ◽  
Basal Cell ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Basal Cell Adenocarcinoma

Flow Cytometric Nuclear DNA Content Analysis of Renal Tumors in Children: Prognostic Significance of Nuclear DNA Ploidy

Tumor Biology ◽  
1995 ◽  
Vol 16 (6) ◽  
pp. 385-393 ◽  
Author(s):  
Pai Li ◽  
Kinji Yokomori ◽  
Yoshiaki Tsuchida ◽  
Masahiro Fujita ◽  
Koichi Shimizu ◽  
...  
Keyword(s):  
Content Analysis ◽  
Dna Content ◽  
Nuclear Dna ◽  
Dna Ploidy ◽  
Prognostic Significance ◽  
Nuclear Dna Content ◽  
Renal Tumors ◽  
Flow Cytometric

Evolutionary DNA variation in Papaver

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 763-768 ◽  
Author(s):  
Sangeeta Srivastava ◽  
U. C. Lavania
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome Length ◽  
Evolutionary Divergence ◽  
Strong Positive Correlation ◽  
Dna Amount ◽  
Dna Variation ◽  
Dna Systematics ◽  
Dna Reduction

In 23 species of Papaver L., 2C nuclear DNA amounts range from 4.64 pg in Papaver persicum (2n = 14) to 22.43 pg in Papaver orientale (2n = 42), revealing a fivefold variation within the genus. However, such variation is limited to only twofold among the species that have the same chromosome number (2n = 14). The distribution of DNA is discontinuously spread over six groups in the genus. A strong positive correlation exists between nuclear DNA content and metaphase chromosome length. Viewed in the context of evolutionary divergence, it is revealed that DNA reduction has taken place in conjunction with speciation. This is achieved by equal reduction to each chromosome independent of chromosome size, as apparent from the estimated DNA values for individual chromosomes within the complements. The diminution in DNA amount with evolutionary specialisation appears to be a genomic strategy to dispense with the less important DNA associated with heterochromatic segments. The uniform distribution of such dispensible DNA throughout the complement is probably nucleotypically conducive to allow the genomic loss to be adaptationally operative, lest it affects the very survival of the evolving species.Key words: Papaver, evolution, DNA content, DNA systematics.

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