Equisetum ×moorei Newman (Equisetaceae) – a ‘new’ nothotaxon in the Polish flora

Abstract To investigate present and historical distribution of Equisetum ×moorei in Poland and its habitat requirements, field studies at sites of potential occurrence of this hybrid taxon as well as literature and herbarium search were performed. As a results of these investigations, E. ×moorei was found at five contemporary and at a few historical localities in the present territory of Poland. Since the Equisetum populations near Olkusz (S Poland) showed phenotype similar, to some extent, to triploid hybrid E. ×ascendens, we performed nuclear DNA content analysis of these populations. However, it turned out that the investigated individuals belonged to a diploid taxon that can be ascribed to E. ×moorei.

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Cytogenetic and Nuclear DNA Content Analysis in Anostomidae Fishes from the Sapucai River, Minas Gerais State, Brazil.

CYTOLOGIA ◽

10.1508/cytologia.67.289 ◽

2002 ◽

Vol 67 (3) ◽

pp. 289-296 ◽

Cited By ~ 4

Author(s):

Mirna Aparecida Pereira ◽

Claudio Oliveira ◽

Fausto Foresti ◽

Edson Luis Maistro

Keyword(s):

Content Analysis ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Minas Gerais ◽

Minas Gerais State

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Polystichum setiferum at the Northeastern Limit of Its Distribution Range

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.902 ◽

2021 ◽

Vol 90 ◽

Author(s):

Dariusz Tlałka ◽

Elwira Sliwinska ◽

Jerzy Kruk

Keyword(s):

Content Analysis ◽

Czech Republic ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Field Studies ◽

Herbarium Specimens ◽

Mountain Range ◽

Species Identity ◽

The Czech Republic ◽

Western Romania

During field studies in 2019 in the Beskid Śląski mountain range in southern Poland, one individual morphologically resembling Polystichum setiferum was found. Nuclear DNA content analysis confirmed the species identity. The new stand of P. setiferum corresponds to historical literature reports and herbarium specimens from the Czech Republic and Poland, and extends the northeastern limit of the distribution of this species. As the historical stands in both countries have not been confirmed, the newly discovered stand is at present strongly isolated, as the nearest extant locations of P. setiferum in Austria, southern Hungary, and western Romania are nearly 500 km away. Therefore, this unique stand deserves special attention and protection.

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Nuclear DNA Content Analysis of Plant Seeds by Flow Cytometry

Current Protocols in Cytometry ◽

10.1002/0471142956.cy0729s35 ◽

2006 ◽

Vol 35 (1) ◽

Cited By ~ 1

Author(s):

Elwira Sliwinska

Keyword(s):

Flow Cytometry ◽

Content Analysis ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Plant Seeds

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Taxonomic remarks on the genus Sternbergia L. (Amaryllidaceae) in Turkey based on leaf anatomy, karyosystematic analysis and nuclear DNA content

Phytotaxa ◽

10.11646/phytotaxa.265.3.4 ◽

2016 ◽

Vol 265 (3) ◽

pp. 238 ◽

Cited By ~ 2

Author(s):

MINE KOÇYIĞIT ◽

METIN TUNA

Keyword(s):

Content Analysis ◽

Cross Sections ◽

Dna Content ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Taxonomic Revision ◽

Chromosome Length ◽

Data Matrix ◽

Vascular Bundles ◽

Taxonomic Markers

Anatomy of leaf cross sections, karyological studies, and nuclear DNA content analysis by flow cytometry were carried out on the six Sternbergia species from Turkey: S. candida (endemic to the country), S. clusiana, S. colchiciflora, S. lutea, S. sicula and S. vernalis. In karyological analysis, number and morphology of the somatic chromosomes, the ploidy level and karyotype formula of the specimens used in the study were investigated in detail. The measurements of somatic chromosomes were calculated with formula of the relative variation in chromosome lenght (CVCL) and mean centromeric asymmetry (MCA). According to the results of this study, there are two different basic chromosome numbers. i.e. x = 10 (S. candida, S. clusiana, S. colchiciflora and S. vernalis) and x = 11 (S. lutea and S. sicula). Results of karyotype analysis corroborated results of previous studies. The smallest chromosome length was measured as 3.05 μm in S. clusiana while the longest length of 20.89 μm was observed in S. sicula. This is the first study reporting nuclear DNA content results for Sternbergia. Based on results of the nuclear DNA content analysis, there is a great variation within the genus in the interspecific level as 2C DNA content varies between 54.03 and 80.42 pg among the species investigated in the study. Anatomical features of the leaves were good taxonomic markers, especially concerning their palisade cells, ribs, cuticle, laticifer canals, vascular bundles, crystals, stomatal index, and stomata structure. Analytical drawings were presented. Six noteworthy leaf characters were quantified, organized in a data matrix, and analysed with UPGMA methods. In conclusion, cytological characteristics of the Sternbergia species together with their leaf features proved to be quite useful in identification and therefore can provide more clarity for taxonomic revision of the genus.

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