Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India

Phytotaxa ◽  
2021 ◽  
Vol 525 (3) ◽  
pp. 205-222
Author(s):  
SANJEET KUMAR VERMA ◽  
SANJAY YADAV ◽  
RAGHVENDRA SINGH
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Murraya Koenigii ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Asexual State

Aplosporella abexaminans, a new sexual morph fungus belongs to family Aplosporellaceae, was discovered on the bark of stem of Murraya koenigii (Rutaceae) and identified by morphological characteristics and analysis of combined ITS and LSU sequence data. This is the second report of a sexual morph with molecular evidence for this genus and the second record of conidiogenesis and chlamydospores associated with the asexual state of this family. It is characterized by its larger ascostromata, locules without ostioles, thinner locule peridium, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, larger and hyaline to light olivaceous ascospores, asexual state without conidiomata formation, conidiogenous cells light brown to brown, conidia brown without granular content, smooth and presence of chlamydospores. The sexual morph of Aplosporella abexaminans resembles Bagnisiella and the asexual morph resembles Aplosporella, thus proving the sexual-asexual connection for the second time for this family.

Additions to Karst Fungi 3: Prosthemium sinense sp nov., from Guizhou Province, China

Phytotaxa ◽  
2016 ◽  
Vol 284 (4) ◽  
pp. 281 ◽  
Author(s):  
HIRAN A. ARIYAWANSA ◽  
KEVIN D. HYDE ◽  
KAZUAKI TANAKA ◽  
SAJEEWA S. N. MAHARACHCHIK-UMBURA ◽  
ABDULLAH M. AL-SADI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South China ◽  
Sequence Data ◽  
Novel Species ◽  
Guizhou Province ◽  
Karst Area ◽  
The Novel ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Size And Morphology

During an assessment of biodiversity of ascomycetous fungi in the South China Karst area in Guizhou Province, China, a novel species of Prosthemium was collected. We isolated the taxon and sequenced the ITS, LSU and TUB gene regions. The novel taxon is characterized by immersed, globose or depressed globose ascomata, flattened, papillate ostioles, cellular pseudoparaphyses, broadly cylindrical to broadly cylindro-clavate asci, narrowly oblong, muriform ascospores and a prosthemium-like asexual morph produced in culture. The result of phylogenetic analysis based on combined ITS, LSU and TUB sequence data indicate that the species belongs to the genus Prosthemium and formed a separate clade within the genus. The new fungus is most similar to Prosthemium betulinum, Pr. intermedium, Pr. neobetulinum and Pr. stellare, from which it differs in the overall smaller size and morphology of sexual morph and asexual morph, as well as phylogeny. Thus new fungus is described and illustrated herein as Prosthemium sinense.

Morphological characteristics and phylogenetic analyses reveal Antrodia yunnanensis sp. nov. (Polyporales, Basidiomycota) from China

Phytotaxa ◽  
2020 ◽  
Vol 460 (1) ◽  
pp. 1-11
Author(s):  
MEI-LING HAN ◽  
QI AN ◽  
WEN-XIAN FU ◽  
XUAN CHENG ◽  
TIAN BU ◽  
...  
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Sister Group ◽  
Southwestern China ◽  
Molecular Evidence ◽  
Pore Surface ◽  
Hyphal System ◽  
Thin Walled

A new polypore, Antrodia yunnanensis, collected from southwestern China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by annual, resupinate basidiocarps with greyish blue to dark greyish blue pore surface upon drying, round to angular pores measuring 2–3 per mm, a dimitic hyphal system with clamped generative hyphae, subicular hyphae bearing fine crystals, and cylindrical, thin-walled, smooth basidiospores measuring 7–9.9 × 2.5–3.1 µm. Molecular phylogeny inferred from ITS and nLSU sequence data showed that samples of A. yunnanensis formed a distinctive lineage in Antrodia sensu lato, and it nested a sister group with A. tropica.

Gibellula flava sp. nov. (Cordycipitaceae, Hypocreales), a new pathogen of spider from China

Phytotaxa ◽  
2021 ◽  
Vol 527 (2) ◽  
pp. 125-133
Author(s):  
MING-JUN CHEN ◽  
TING WANG ◽  
YAN LIN ◽  
BO HUANG
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Anhui Province ◽  
Molecular Evidence ◽  
Asexual Morph ◽  
Phylogenetic Placement ◽  
Sexual Morph ◽  
Conidial State ◽  
A New Species

Gibellula is an exclusively spider-pathogen genus, with most species immediately recognized by their distinctive conidial state, but also including the teleomorphic states of these fungi that were previously included in the rejected genus Torrubiella. Two sexual specimens and an asexual morph specimen were collected from Anhui Province, China, and were recognized as the same species based on molecular evidence. Its asexual morph differs from Gibellula pulchra, to which it is morphologically similar, in having shorter verrucose conidiophores with slightly smaller metulae, phialides and conidia. Its sexual morph is characterized by producing superficial and elongate ellipsoid small perithecia with short asci on a cream-yellow mycelial mat covering the host. Multi-locus (SSU, LSU, TEF, and RPB1) phylogenetic analyses showed that the specimens belong to a strongly supported subclade, and formed a sister subclade with G. pulchra and Gibellula sp. A combination of morphological characteristics and its phylogenetic placement confirmed that these unique specimens are a new species, which is described as Gibellula flava.

Tetraploa aquatica (Tetraplosphaeriaceae), a new freshwater fungal species from Yunnan Province, China

Phytotaxa ◽  
2020 ◽  
Vol 459 (2) ◽  
pp. 181-189
Author(s):  
WEN-LI LI ◽  
DAN-FENG BAO ◽  
D. JAYARAMA BHAT ◽  
HONG-YAN SU
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Fungal Species ◽  
Asexual Morph ◽  
Data Support ◽  
Decaying Wood

Asexual morph members of Tetraplosphaeriaceae (Pleosporales, Dothideomycetes) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. Two isolates of a novel species, Tetraploa aquatica sp. nov., was collected from submerged decaying wood in Dulong river and a stream of Gaoligongshan mountain in Yunnan Province, China. The new species is characterized by short-cylindrical conidia, composed of 4-euseptate, short-cylindrical vertical columns which are verrucose at the base and with 4-setose vertical septate appendages. Tetraploa aquatica can be easily distinguished from other Tetraploa species by their columns which are 2–3-septate, aguttulate, vertical, setose appendaged which tend to remain parallel to one another apically. Phylogenetic analyses of combined LSU, ITS and SSU sequence data support its natural placement in Tetraploa. Detailed description and illustrations of this species and comparisons with other morphologically similar taxa in Tetraploa are provided.

Molecular phylogeny and morphology of Cunninghamella guizhouensis sp. nov. (Cunninghamellaceae, Mucorales), from soil in Guizhou, China

Phytotaxa ◽  
2020 ◽  
Vol 455 (1) ◽  
pp. 31-39
Author(s):  
ZHI-YUAN ZHANG ◽  
YI-XUAN ZHAO ◽  
XIN SHEN ◽  
WAN-HAO CHEN ◽  
YAN-FENG HAN ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Keratinolytic Fungi ◽  
A New Species ◽  
Shape And Size

During a survey of keratinolytic fungi in China, three Cunninghamella strains were isolated. Phylogenetic analyses of ITS and ITS+LSU+EF-1α sequence data showed that these strains constitute a new species related to C. blakesleeana, C. bigelovii, C. multiverticillata and C. phaeospora. The new species differs from C. multiverticillata and C. phaeospora in the shape and size of its teminal and lateral vesicles and can be distinguished from C. blakesleeana and C. bigelovii by the absent of zygosporangia, and the shape and size of it sporangioles. The results of phylogenetic and morphological analyses indicate that the three strains are a new species of Cunninghamella. Descriptions and illustrations of this novel species are provided in this paper.

Xanthagaricus siamensis sp. nov. (Agaricaceae), a new species with dull green lamellae from northern Thailand

Phytotaxa ◽  
2020 ◽  
Vol 437 (1) ◽  
pp. 14-22
Author(s):  
YUAN S. LIU ◽  
JIAN-KUI (JACK) LIU ◽  
PHONGEUN SYSOUPHANTHONG ◽  
KEVIN D. HYDE ◽  
SAISAMORN LUMYONG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Molecular Evidence ◽  
Internal Transcribed Spacer Region ◽  
Northern Thailand ◽  
A New Species

Xanthagaricus siamensis, a new species in the family Agaricaceae, was discovered in northern Thailand and is here introduced based on its morphological features and molecular data. It is characterized by small to medium-sized basidiomata, a convex to plano-convex with depressed center pileus when mature, the presence of greyish orange to violet-brown fibrillose squamules on the pileus, lamellae that start out white, change to pinkish white when damaged, then change to dull green with age. Additionally, the annulus is fugacious, and the pileipellis is recognized as a cutis which morphologically distinguishes it from all other known Xanthagaricus. Phylogenetic analysis of the internal transcribed spacer region (ITS) and the large subunit of the nuclear ribosomal DNA (nrLSU) sequence data indicated that the three specimens of X. siamensis form a distinct lineage within Xanthagaricus, and they formed a well-supported clade representing the genus Xanthagaricus. Detailed illustrations of macro- and micro-morphological characteristics and descriptions are provided, as well as other relevant molecular evidence.

Ophiostoma pehueninum, a new species associated with Araucaria araucana in Chile

Phytotaxa ◽  
2018 ◽  
Vol 346 (1) ◽  
pp. 93
Author(s):  
MARIO ZAPATA ◽  
MARIA ANTONIETA PALMA ◽  
EDUARDO PIONTELLI
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Andean Region ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Mating Compatibility ◽  
Araucaria Araucana ◽  
A New Species ◽  
Β Tubulin

During a survey of fungi on Araucaria araucana in Chile, a species of Ophiostoma morphologically different to O. araucariae was collected in the Andean region. Morphological characteristics and phylogenetic analyses of ITS, β-tubulin and EF-1α sequences confirmed that this species represents a novel taxon. This taxon is described here as Ophiostoma pehueninum sp. nov. The sexual morph of O. pehueninum was characterized by long neck and peridial hyphae around the bases, and allantoid ascospores surrounded by a gelatinous sheath. In culture a hyalorhinocladiella-like asexual morph was observed. Mating compatibility tests indicated that the species is heterothallic.

scholarly journals Two novel species and two new records of Distoseptispora from freshwater habitats in China and Thailand

MycoKeys ◽  
2021 ◽  
Vol 84 ◽  
pp. 79-101
Author(s):  
Hong-Wei Shen ◽  
Dan-Feng Bao ◽  
Kevin D. Hyde ◽  
Hong-Yan Su ◽  
Darbhe J. Bhat ◽  
...  
Keyword(s):  
New Records ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Freshwater Fungi ◽  
Freshwater Habitat ◽  
Two New Species ◽  
Freshwater Habitats ◽  
Great Mekong Subregion

During investigations into freshwater fungi from the Great Mekong Subregion, four Distoseptispora taxa were collected from China and Thailand. Based on morphological characteristics, and phylogenetic analyses of combined LSU, ITS, SSU, TEF1-α, and RPB2 sequence data, two new species Distoseptispora bangkokensis and D. lancangjiangensis are introduced, and two known species D. clematidis and D. thysanolaenae were first reported in freshwater habitat. Illustrations and descriptions of these taxa are provided, along with comparisons with extant taxa in the genus.

scholarly journals Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. https://doi.org/10.3897/mycokeys.61.46446

MycoKeys ◽  
2020 ◽  
Vol 63 ◽  
pp. 163-172
Author(s):  
Xiao-Lan He ◽  
Egon Horak ◽  
Di Wang ◽  
Tai-Hui Li ◽  
Wei-Hong Peng ◽  
...  
Keyword(s):  
New Species ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Novel Species ◽  
Molecular Data ◽  
Monophyletic Group ◽  
Molecular Evidence ◽  
Morphological And Molecular Data ◽  
High Level ◽  
Ecological Range

Entoloma subgenus Claudopus is widely distributed, yet the taxonomy and systematics of its species are still poorly documented. In the present study, more than forty collections of Claudopus were gathered in China and subsequently analysed, based on morphological and molecular data. The results revealed first a high level of species diversity of Claudopus in China and second, there is a wide ecological range regarding the substrates and the habitats ranging from temperate, tropical to subalpine locations. Based on morphological and molecular evidence, five novel species from China are proposed, viz. E. conchatum, E. flabellatum, E. gregarium, E. pleurotoides and E. reductum. Molecular phylogeny of Entoloma s.l. was also reconstructed, based on 187 representatives of Entoloma s.l. by employing the combined ITS, LSU, mtSSU and RPB2 sequences. Ten monophyletic clades (Claudopus, Leptonia, Nolanea, Cuboid-spored Inocephalus, “Alboleptonia”, Cyanula, Pouzarella, Rhodopolia, Prunuloides and Rusticoides) were recovered, while 13 taxa could not be placed in any defined clades. The results confirmed that Claudopus in a traditional morphological sense is not monophyletic and the Rusticoides-group, previously considered within Claudopus, formed a separate clade; but section Claudopus and relatives of E. undatum belong to a distinctive monophyletic group. Despite some monophyletic groups in Entoloma s.l. being distinctive in both morphology and molecular phylogeny, they were still treated as subgenera of Entoloma s.l. temporarily, because accepting them as genera will make Entoloma s.l. paraphyletic.

scholarly journals Additions to the Genus Arthrinium (Apiosporaceae) From Bamboos in China

2021 ◽  
Vol 12 ◽  
Author(s):  
Yao Feng ◽  
Jian-Kui (Jack) Liu ◽  
Chuan-Gen Lin ◽  
Ya-Ya Chen ◽  
Mei-Mei Xiang ◽  
...  
Keyword(s):  
New Species ◽  
Marine Algae ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Human Tissues ◽  
Widespread Distribution ◽  
Phylogenetic Characterization ◽  
Asexual Morph ◽  
Sexual Morph ◽  
First Time

Arthrinium has a widespread distribution occurring in various substrates (e.g., air, soil debris, plants, lichens, marine algae and even human tissues). It is characterized by the basauxic conidiogenesis in the asexual morph, with apiospores in the sexual morph. In this study, seventeen isolates of Arthrinium were collected in China. Based on their morphology and phylogenetic characterization, four new species (A. biseriale, A. cyclobalanopsidis, A. gelatinosum, and A. septatum) are described and seven known species (A. arundinis, A. garethjonesii, A. guizhouense, A. hydei, A. neosubglobosa, A. phyllostachium and A. psedoparenchymaticum) are identified, of which the sexual morph of three species (A. guizhouense, A. phyllostachium and A. psedoparenchymaticum) and asexual morph of A. garethjonesii are reported for the first time. The detailed descriptions, illustrations and comparisons with related taxa of these new collections are provided. Phylogenetic analyses of combined ITS, LSU, TUB2, and TEF sequence data support their placements in the genus Arthrinium and justify the new species establishments and identifications of known species.

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