Additions to Karst Fungi 3: Prosthemium sinense sp nov., from Guizhou Province, China

Phytotaxa ◽  
2016 ◽  
Vol 284 (4) ◽  
pp. 281 ◽  
Author(s):  
HIRAN A. ARIYAWANSA ◽  
KEVIN D. HYDE ◽  
KAZUAKI TANAKA ◽  
SAJEEWA S. N. MAHARACHCHIK-UMBURA ◽  
ABDULLAH M. AL-SADI ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
South China ◽  
Sequence Data ◽  
Novel Species ◽  
Guizhou Province ◽  
Karst Area ◽  
The Novel ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Size And Morphology

During an assessment of biodiversity of ascomycetous fungi in the South China Karst area in Guizhou Province, China, a novel species of Prosthemium was collected. We isolated the taxon and sequenced the ITS, LSU and TUB gene regions. The novel taxon is characterized by immersed, globose or depressed globose ascomata, flattened, papillate ostioles, cellular pseudoparaphyses, broadly cylindrical to broadly cylindro-clavate asci, narrowly oblong, muriform ascospores and a prosthemium-like asexual morph produced in culture. The result of phylogenetic analysis based on combined ITS, LSU and TUB sequence data indicate that the species belongs to the genus Prosthemium and formed a separate clade within the genus. The new fungus is most similar to Prosthemium betulinum, Pr. intermedium, Pr. neobetulinum and Pr. stellare, from which it differs in the overall smaller size and morphology of sexual morph and asexual morph, as well as phylogeny. Thus new fungus is described and illustrated herein as Prosthemium sinense.

Conioscypha tenebrosa sp. nov. (Conioscyphaceae) from China and notes on Conioscypha species

Phytotaxa ◽  
2019 ◽  
Vol 413 (2) ◽  
pp. 159-171
Author(s):  
NING-GUO LIU ◽  
D. JAYARAMA BHAT ◽  
KEVIN D. HYDE ◽  
JIAN-KUI (JACK) LIU
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Sequence Data ◽  
Guizhou Province ◽  
New Taxon ◽  
Morphological Comparison ◽  
Asexual Morph ◽  
Rounded Apex ◽  
The Family ◽  
Decaying Wood

Conioscypha is an asexual morph genus placed in the family Conioscyphaceae. During our study of brown-spored hyphomycetes, a new taxon C. tenebrosa was found on decaying wood collected in Guizhou Province, China. The new species is characterized by having micronematous, hyaline conidiophores which are often reduced to conidiogenous cells, with a cup-shaped, percurrently developed, multi-collaretted phialide, and globose to subglobose, obovoid conidia with broadly rounded apex and subtruncate base. The phylogenetic analysis of combined LSU, SSU, ITS and RPB2 sequence data showed that isolates of C. tenebrosa are phylogenetically distinct from other species. Conioscypha tenebrosa sp. nov. is therefore introduced here with a description and morphological illustration. Taxonomic notes and a morphological comparison of Conioscypha species are provided.

Molecular phylogeny of Aplosporella abexaminans: a novel species revealing the second report of sexual-asexual connection in Aplosporellaceae (Botryosphaeriales) from India

Phytotaxa ◽  
2021 ◽  
Vol 525 (3) ◽  
pp. 205-222
Author(s):  
SANJEET KUMAR VERMA ◽  
SANJAY YADAV ◽  
RAGHVENDRA SINGH
Keyword(s):  
Molecular Phylogeny ◽  
Sequence Data ◽  
Novel Species ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Murraya Koenigii ◽  
Asexual Morph ◽  
Sexual Morph ◽  
Asexual State

Aplosporella abexaminans, a new sexual morph fungus belongs to family Aplosporellaceae, was discovered on the bark of stem of Murraya koenigii (Rutaceae) and identified by morphological characteristics and analysis of combined ITS and LSU sequence data. This is the second report of a sexual morph with molecular evidence for this genus and the second record of conidiogenesis and chlamydospores associated with the asexual state of this family. It is characterized by its larger ascostromata, locules without ostioles, thinner locule peridium, two to multi-layered, larger asci, surrounded by an additional hyaline sac like structure, larger and hyaline to light olivaceous ascospores, asexual state without conidiomata formation, conidiogenous cells light brown to brown, conidia brown without granular content, smooth and presence of chlamydospores. The sexual morph of Aplosporella abexaminans resembles Bagnisiella and the asexual morph resembles Aplosporella, thus proving the sexual-asexual connection for the second time for this family.

Additions to Karst Fungi 2: Alpestrisphaeria jonesii from Guizhou Province, China

Phytotaxa ◽  
2016 ◽  
Vol 277 (3) ◽  
pp. 255 ◽  
Author(s):  
HIRAN A. ARIYAWANSA ◽  
KEVIN D. HYDE ◽  
KASUN M. THAMBUGALA ◽  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
ABDULLAH M. AL-SADI ◽  
...  
Keyword(s):  
Dead Wood ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Guizhou Province ◽  
Karst Area ◽  
New Taxon ◽  
The Novel ◽  
Diagnostic Features ◽  
Generic Type ◽  
The Family

Lophiostomataceae (Pleosporales, Dothideomycetes) is a ubiquitous family that includes saprobic species associated with a wide variety of substrates in various habitats. DNA based studies have shown numerous genera can be determined within the family Lophiostomataceae that do not always connect to species based on morphological characteristics. An undescribed ascomycete, similar to species of Lophiostomataceae, was collected from dead wood in the South China karst area in Guizhou Province. Phylogenetic analysis of ITS, SSU, LSU and EF1α sequence data revealed that the new taxon nested in the genus Alpestrisphaeria in Lophiostomataceae, thus the novel taxon is introduced as Alpestrisphaeria jonesii. Diagnostic features are erumpent, coriaceous to carbonaceous, subglobose ascomata, a slit-like ostiole with periphyses, filiform pseudoparaphyses, fissitunicate, cylindrical asci and filamentous, 10–15-euseptate, hyaline ascospores. Alpestrisphaeria jonesii can be readily distinguished from the generic type of Alpestrisphaeria, A. terricola by the shape of its asci and ascospores.

Steinernema khuongin. sp. (Panagrolaimomorpha, Steinernematidae), a new entomopathogenic nematode species from Florida, USA

2018 ◽  
Vol 93 (2) ◽  
pp. 226-241 ◽  
Author(s):  
S.P. Stock ◽  
R. Campos-Herrera ◽  
F.E. El-Borai ◽  
L.W. Duncan
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Novel Species ◽  
Entomopathogenic Nematode ◽  
Biological Species ◽  
Morphological Data ◽  
Biological Species Concept ◽  
The Novel ◽  
Ecological Data ◽  
Cross Hybridization

AbstractIn this study, molecular (ribosomal sequence data), morphological and cross-hybridization properties were used to identify a newSteinernemasp. from Florida, USA. Molecular and morphological data provided evidence for placing the novel species into Clade V, or the ‘glaseri-group’ ofSteinernemaspp. Within this clade, analysis of sequence data of the rDNA genes, 28S and internal transcribed spacer (ITS), depicted the novel species as a distinctive entity and closely related toS. glaseriandS. cubanum.Additionally, cross-hybridization assays showed that the new species is unable to interbreed with either of the latter two species, reinforcing its uniqueness from a biological species concept standpoint. Key morphological diagnostic characters forS. khuongin. sp. include the mean morphometric features of the third-stage infective juveniles: total body length (average: 1066 μm), tail length (average: 65 μm), location of the excretory pore (average: 80.5 μm) and the values ofc(average: 16.4),D% (average: 60.5),E% (average: 126) andH% (average: 46.6). Additionally, males can be differentiated fromS. glaseriandS. cubanumby the values of several ratios:D% (average: 68),E% (average: 323) and SW% (average: 120). The natural distribution of this species in Florida encompasses both natural areas and citrus groves, primarily in shallow groundwater ecoregions designated as ‘flatwoods’. The morphological, molecular, phylogenetic and ecological data associated with this nematode support its identity as a new species in theS. glaseri-group.

Tetraploa aquatica (Tetraplosphaeriaceae), a new freshwater fungal species from Yunnan Province, China

Phytotaxa ◽  
2020 ◽  
Vol 459 (2) ◽  
pp. 181-189
Author(s):  
WEN-LI LI ◽  
DAN-FENG BAO ◽  
D. JAYARAMA BHAT ◽  
HONG-YAN SU
Keyword(s):  
New Species ◽  
Yunnan Province ◽  
Sequence Data ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Fungal Species ◽  
Asexual Morph ◽  
Data Support ◽  
Decaying Wood

Asexual morph members of Tetraplosphaeriaceae (Pleosporales, Dothideomycetes) are generally hyphomycetes and usually found as saprobes occurring on various hosts in lentic habitats. Two isolates of a novel species, Tetraploa aquatica sp. nov., was collected from submerged decaying wood in Dulong river and a stream of Gaoligongshan mountain in Yunnan Province, China. The new species is characterized by short-cylindrical conidia, composed of 4-euseptate, short-cylindrical vertical columns which are verrucose at the base and with 4-setose vertical septate appendages. Tetraploa aquatica can be easily distinguished from other Tetraploa species by their columns which are 2–3-septate, aguttulate, vertical, setose appendaged which tend to remain parallel to one another apically. Phylogenetic analyses of combined LSU, ITS and SSU sequence data support its natural placement in Tetraploa. Detailed description and illustrations of this species and comparisons with other morphologically similar taxa in Tetraploa are provided.

Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia)

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 1179-1191 ◽  
Author(s):  
Wen Song ◽  
Jiamei Li ◽  
Weiwei Liu ◽  
Jiamei Jiang ◽  
Khaled A. S. Al- Rasheid ◽  
...  
Keyword(s):  
Sequence Data ◽  
Novel Species ◽  
Small Subunit ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
The Novel ◽  
Small Subunit Rrna ◽  
Ssu Rrna Gene ◽  
Single Clade ◽  
Ssu Rrna Gene Sequence

Three oligotrich ciliates, Apostrombidium parakielum spec. nov., Novistrombidium apsheronicum (Alekperov & Asadullayeva, 1997) Agatha, 2003 and Novistrombidium testaceum (Anigstein, 1914) Song & Bradbury, 1998 were collected from the coastal waters of China and their morphology and small-subunit rRNA (SSU rRNA) gene sequences were studied. The novel species can be recognized by the combination of its obconical body shape, 14–16 anterior and 6–8 ventral membranelles, somatic kinety in three parts and conspicuously long dorsal cilia. Based on the data obtained for this novel species, an improved diagnosis of the genus Apostrombidium is supplied. Descriptions of the population of N. apsheronicum and N. testaceum collected in this study are also provided and compared with the existing descriptions. In addition, the phylogenetic positions of these three species are inferred from their SSU rRNA gene sequence data. The results indicate that the genus Apostrombidium, the systematics of which has not previously been discussed using molecular information, clusters with Varistrombidium kielum and Omegastrombidium elegans, whereas N. testaceum and N. apsheronicum form a single clade.

scholarly journals Lewinella xylanilytica sp. nov., a member of the family Saprospiraceae isolated from coastal seawater

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3433-3438 ◽  
Author(s):  
Hye-Ri Sung ◽  
Jin-Man Lee ◽  
Mibang Kim ◽  
Kee-Sun Shin
Keyword(s):  
Fatty Acids ◽  
Phylogenetic Analysis ◽  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Unidentified Phospholipid ◽  
Gram Staining ◽  
The Family

An orange-pigmented bacterium, designated strain 13-9-B8T, was isolated from a seawater sample collected at Marado, Jeju Island, South Korea. The novel strain was Gram-staining-negative, non-motile, non-gliding, rod-shaped and aerobic. A phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain clustered with members of the genus Lewinella of the family Saprospiraceae in the phylum Bacteroidetes and was most closely related to the species Lewinella marina (95.6 % similarity to the type strain). Strain 13-9-B8T grew optimally at 30 °C, pH 7.0 and with 2 % (w/v) NaCl. Strain 13-9-B8T contained MK-7 as the predominant menquinone and summed feature 3, iso-C15 : 0 and iso-C17 : 0 3-OH as the major fatty acids. The polar lipids detected in strain 13-9-B8T were phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and eight unidentified lipids. The DNA G+C content of strain 13-9-B8T was 59.1 mol%. Based on phenotypic, chemotaxonomic and phylogenetic data presented, strain 13-9-B8T is considered to represent a novel species of the genus Lewinella, for which the name Lewinella xylanilytica sp. nov. is proposed. The type strain is 13-9-B8T ( = DSM 29526T = KCTC 32663T).

scholarly journals Delimitation of a novel member of genus Metarhizium (Clavicipitaceae) by phylogenetic and network analysis

Phytotaxa ◽  
2016 ◽  
Vol 288 (1) ◽  
pp. 51 ◽  
Author(s):  
HUA-LI CHU ◽  
WAN-HAO CHEN ◽  
TING-CHI WEN ◽  
ZONG-QI LIANG ◽  
FU-CHONG ZHENG ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Network Analysis ◽  
Sequence Data ◽  
Novel Species ◽  
Its Sequence ◽  
Wetland Area ◽  
Mucilaginous Sheath ◽  
Guiyang City

An entomopathogenic taxon from a wetland area in Guiyang City, China was found to be a novel species of Metarhizium based on both morphology and phylogeny. The new species, which has unique, slender and cylindrical, Akanthomyces-like synnemata is described, illustrated and named Metarhizium synnematis. Phylogenetic analysis of ITS sequence data confirmed that M. synnematis belongs in Metarhizium. It differs from other species of Metarhizium in having relatively small, ellipsoidal to cylindrical or sub-clavate conidia, coated with a thin mucilaginous sheath which aggregates spores into sticky masses.

scholarly journals Lactobacillus paracollinoides sp. nov., isolated from brewery environments

2004 ◽  
Vol 54 (1) ◽  
pp. 115-117 ◽  
Author(s):  
Koji Suzuki ◽  
Wataru Funahashi ◽  
Masahiro Koyanagi ◽  
Hiroshi Yamashita
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
The Novel ◽  
Rrna Gene Sequence ◽  
Dna Relatedness ◽  
Beer Spoilage

Three novel strains isolated from brewery environments are described. These strains were Gram-positive, facultatively anaerobic, heterofermentative rods that did not exhibit catalase activity. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that these strains belong to the genus Lactobacillus and are most closely related to Lactobacillus collinoides (approximately 99 % similarity). The novel strains could be differentiated from L. collinoides on the basis of DNA–DNA relatedness, differences in beer-spoilage ability and the inability to utilize d-fructose. These isolates represent a novel species, for which the name Lactobacillus paracollinoides sp. nov. is proposed. The type strain is LA2T (=DSM 15502T=JCM 11969T).

scholarly journals Two novel species of Neoaquastroma (Parabambusicolaceae, Pleosporales) with their phoma-like asexual morphs

MycoKeys ◽  
2018 ◽  
Vol 34 ◽  
pp. 47-62 ◽  
Author(s):  
Chayanard Phukhamsakda ◽  
Darbhe J. Bhat ◽  
Sinang Hongsanan ◽  
Jian-Chu Xu ◽  
Marc Stadler ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Taxa ◽  
Type Species ◽  
Sequence Data ◽  
Novel Species ◽  
Monotypic Genus ◽  
Dicotyledonous Plant ◽  
The Family ◽  
Distinct Lineage ◽  
Asexual Morphs

The monotypic genus Neoaquastroma (Parabambusicolaceae, Pleosporales) was introduced for a microfungus isolated from a collection of dried stems of a dicotyledonous plant in Thailand. In this paper, we introduce two novel species, N.bauhiniae and N.krabiense, in this genus. Their asexual morphs comprise conidiomata with aseptate and hyaline conidia. Neoaquastromabauhiniae has ascomata, asci and ascospores that are smaller than those of N.krabiense. Descriptions and illustrations of N.bauhiniae and N.krabiense are provided and the two species compared with the type species of the genus, N.guttulatum. Evidence for the introduction of the new taxa is also provided from phylogenetic analysis of a combined dataset of partial LSU, SSU, ITS and tef1 sequence data. The phylogenetic analysis revealed a distinct lineage for N.bauhiniae and N.krabiense within the family Parabambusicolaceae.

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