Placement of Cacoceria and phylogenetic relationships of the xylotine genera of the tribe Milesiini (Diptera, Syrphidae: Eristalinae) based on molecular characters

Zootaxa ◽  
2006 ◽  
Vol 1171 (1) ◽  
pp. 17 ◽  
Author(s):  
GUNILLA STÅHLS
Keyword(s):  
Intraspecific Variation ◽  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Large Fragment ◽  
Mitochondrial Coi ◽  
Phylogenetic Placement ◽  
28S Rrna Gene ◽  
Molecular Characters

The phylogenetic relationships of traditional xylotine genera of tribe Milesiini (Diptera, Syrphidae: Eristalinae) were explored using molecular character of a large fragment of the mitochondrial COI and the D2-3 region of the nuclear ribosomal 28S rRNA gene. Of particular interest was the phylogenetic placement of the enigmatic Neotropical genus Cacoceria. The ingroup included 48 taxa, and Cheilosia illustrata (Rhingiini, Eristalinae) was used as outgroup. Multiple specimens of more common taxa were sequenced for surveying levels of intraspecific variation. The combined dataset was analysed using parsimony and optimisation alignment, using the program POY. Cacoceria was resolved within the Chalcosyrphus clade. Earlier hypotheses placed the taxon in the tribe Myoleptini or Chrysogasterini, or in Xylotini without stating a closer relationship to any particular xylotine taxon. The representatives of traditional xylotine taxa were resolved as ((Hadromyia + (Brachypalpoides + Blera + Lejota) + (Xylota sg. Hovaxylota + Xylota sg. Sterphoides) + (Xylota (including Sterphus)) while Neplas and Brachypalpus were resolved in the Myoleptini albeit with very low support.

Phylogenetic relationships of Telotylenchidae Siddiqi, 1960 and Merliniidae Siddiqi, 1971 (Nematoda: Tylenchida) from Iran, as inferred from the analysis of the D2D3 expansion fragments of 28S rRNA gene sequences

Nematology ◽  
2014 ◽  
Vol 16 (7) ◽  
pp. 863-877 ◽  
Author(s):  
Reza Ghaderi ◽  
Akbar Karegar ◽  
Gholamreza Niknam ◽  
Sergei A. Subbotin
Keyword(s):  
Phylogenetic Relationships ◽  
Gene Sequence ◽  
Rrna Gene ◽  
28S Rrna ◽  
Single Family ◽  
Rrna Gene Sequence ◽  
Gene Sequences ◽  
28S Rrna Gene ◽  
Likelihood Analysis ◽  
Large Genus

Sixteen species,Amplimerlinius globigerus,A. macrurus,Bitylenchus parvus,Merlinius brevidens,M. nanus,Neodolichorhynchus phaseoli,Paramerlinius neohexagrammus,Pratylenchoides alkani,P. ritteri,P. utahensis,Scutylenchus paniculoides,S. rugosus,S. tartuensis,Scutylenchussp. A,Trophurus imparandTylenchorhynchus brassicae, from the families Telotylenchidae and Merliniidae were collected from different locations in Iran and molecularly characterised using sequencing of the D2D3 expansion fragments of the 28S rRNA gene. Morphometrics and light micrography for studied species are also provided as vouchers. The phylogenetic relationships of Telotylenchidae and Merliniidae with other representatives of the order Tylenchida, as obtained from Bayesian inference and Maximum likelihood analysis of partial 28S rRNA gene sequences, are presented and discussed. The results of phylogenetic analysis were in accordance with classifications in whichBitylenchusandScutylenchusare considered as separate genera, butTessellusandTelotylenchuswere synonyms ofTylenchorhynchus. The Shimodaira-Hasegawa test of the 28S rRNA gene sequence alignment and trees rejected a large genus concept ofTylenchorhynchusand the constrained monophyly for Belonolaimidae revealed within this family two genera groups:i) BelonolaimusandIbipora; andii) CarphodorusandMorulaimus. The present results also support the combination ofPratylenchoidesand Merliniinae into a single family, the Merliniidae.

Descriptions of Trichodorus lownsberyi sp. n., Nanidorus minor (Colbran, 1956) Siddiqi, 1974 (Nematoda: Trichodoridae) and Longidorus quercus sp. n. (Nematoda: Longidoridae) from Mexico

Nematology ◽  
2021 ◽  
pp. 1-15
Author(s):  
Ignacio Cid del Prado Vera ◽  
Sergei A. Subbotin ◽  
Wilfrida Decraemer
Keyword(s):  
New Species ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
The Body ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
28S Rrna Gene ◽  
Buxus Sempervirens ◽  
Stubby Root Nematode

Summary A new species of stubby root nematode, Trichodorus lownsberyi sp. n., collected from soil around Buxus sempervirens at Montecillo Campus, Colegio de Postgraduados, Mexico, is described and illustrated. Trichodorus lownsberyi sp. n. is characterised by the abundant males with a characteristic spicule shape, the narrower part about mid-blade having visible bristles in most specimens, and the presence and position of the three ventromedian cervical papillae, which are all anterior to the secretory-excretory pore. Females possess a rhomboid-shaped vagina and oval to rounded triangular vaginal sclerotised pieces with a pore-like vulva. The phylogenetic relationships of T. lownsberyi sp. n. with related species were constructed using the ITS2 rRNA and the D2-D3 expansion segments of 28S rRNA gene sequences. Trichodorus lownsberyi sp. n. was a sister species to T. viruliferus in the phylogenetic trees. A Mexican population of Nanidorus minor from a peach orchard is also described and illustrated. The new needle nematode, Longidorus quercus sp. n., was recovered from soil around roots of oak, Quercus crassipes, from Cerro Jusda ‘El Diablo’ in Mexico State. Longidorus quercus sp. n. females are characterised by the C-shaped posterior end of the body after fixation, L = 4.9 (3.9-5.6) mm, a = 71 (60.8-93.2), lip region rounded, sometimes slightly flattened, marked by depression, odontostylet 170 (144-206) μm long, hemispherical to bluntly conoid tail and no males. The new species was characterised using the D2-D3 of 28S rRNA and COI gene sequences. The phylogenetic relationships of L. quercus sp. n. with other Longidorus species were reconstructed using the D2-D3 of 28S rRNA gene sequences.

scholarly journals Phylogenetic relationships of conifers inferred from Partial 28S rRNA gene sequences

1998 ◽  
Vol 85 (5) ◽  
pp. 688-697 ◽  
Author(s):  
Saša Stefanović ◽  
Muriel Jager ◽  
Jean Deutsch ◽  
Jean Broutin ◽  
Monique Masselot
Keyword(s):  
Phylogenetic Relationships ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
28S Rrna Gene

Conioscypha peruviana sp. nov., its phylogenetic placement based on 28S rRNA gene, and a report of Conioscypha gracilis comb. nov. from Peru

Mycoscience ◽  
2015 ◽  
Vol 56 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Steven E. Zelski ◽  
Huzefa A. Raja ◽  
Andrew N. Miller ◽  
Carol A. Shearer
Keyword(s):  
Rrna Gene ◽  
28S Rrna ◽  
Phylogenetic Placement ◽  
28S Rrna Gene

Characterisation of populations of Heterodera trifolii Goffart, 1932 (Nematoda: Heteroderidae) in Japan and their phylogenetic relationships with closely related species

Nematology ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. 543-558
Author(s):  
Shigeyuki Sekimoto ◽  
Taketo Uehara ◽  
Takayuki Mizukubo
Keyword(s):  
Related Species ◽  
Phylogenetic Relationships ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Closely Related Species ◽  
28S Rrna Gene ◽  
Intraspecific Variations ◽  
Low Levels

The Japanese populations of the clover cyst nematode,Heterodera trifolii, were characterised morphologically, morphometrically and molecularly. The morphology and morphometrics of six Japanese populations ofH. trifoliiwere in congruence with those of previous descriptions of this species from Germany, Italy, Japan, New Zealand, Russia, The Netherlands, UK and USA. The results of the phylogenetic analyses of the D2-D3 expansion segments of 28S rRNA gene and theCOImtDNA gene sequences discriminatedH. trifoliifrom most of the other closely related species of theSchachtiigroup. The intraspecific sequence differences in theSchachtiigroup were much higher inCOIthan in D2-D3. TheH. trifoliipopulations displayed very low levels of intraspecific variations (up to 0.3%) in theCOImtDNA gene sequences. The phylogenetic relationships ofH. trifoliiwith otherHeteroderaspecies, as inferred from the analyses of the ITS rRNA gene, D2-D3 of 28S rRNA gene andCOImtDNA gene sequences, were determined.

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