The tadpole of Ecnomiohyla sukia Savage & Kubicki, 2010 (Amphibia: Hylidae)

Zootaxa ◽  
2011 ◽  
Vol 2793 (1) ◽  
pp. 63
Author(s):  
HEINZ HOFFMANN ◽  
BRIAN KUBICKI
Keyword(s):  
Costa Rica ◽  
Larval Development ◽  
Tree Frog ◽  
Adult Frog ◽  
Tree Hole ◽  
Adult Tree ◽  
The Village

Near the village of Guayacán de Siquirres, Limón province, Costa Rica (N 10°02”, W 83°31’) the call of a frog revealed a breeding hole about 15 m high in a Guarea tree. An adult was taken from this water-filled cavity along with some tadpoles. The adult tree frog was identified by B. Kubicki as Ecnomiohyla sukia Savage and Kubicki, 2010. Its sex was not determined. Ten tadpoles were extracted from the water-filled tree hole and it appeared they had only a few days of larval development, but the exact age was undeterminable. The conspecificity of the tadpoles found with the adult frog E. sukia was established by rearing them to metamorphic stages and froglets. The following description is based on tadpoles from stages 25, 36–37, and 41 (Gosner 1960).

A New Tree-Frog (Centrolenidae) from Costa Rica

Copeia ◽  
1967 ◽  
Vol 1967 (2) ◽  
pp. 325 ◽  
Author(s):  
Jay M. Savage
Keyword(s):  
Costa Rica ◽  
Tree Frog

Amphibian thalamic nuclear organization during larval development and in the adult frog Xenopus laevis : Genoarchitecture and hodological analysis

2020 ◽  
Vol 528 (14) ◽  
pp. 2361-2403
Author(s):  
Ruth Morona ◽  
Sandra Bandín ◽  
Jesús M. López ◽  
Nerea Moreno ◽  
Agustín González
Keyword(s):  
Xenopus Laevis ◽  
Larval Development ◽  
Nuclear Organization ◽  
Adult Frog

scholarly journals First extraction of eDNA from tree hole water to detect tree frogs: a simple field method piloted in Madagascar

2021 ◽  
Author(s):  
Katherine E. Mullin ◽  
Izabela M. Barata ◽  
Jeff Dawson ◽  
Pablo Orozco-terWengel
Keyword(s):  
Cryptic Species ◽  
Environmental Dna ◽  
Cost Effective ◽  
Field Method ◽  
Tree Frog ◽  
Endangered Tree ◽  
Small Water ◽  
Tree Hole ◽  
Two Samples ◽  
Tree Holes

AbstractEnvironmental DNA (eDNA) is becoming an increasingly used tool for monitoring cryptic species within terrestrial and aquatic systems. We present the first method for extracting water from tree holes for eDNA studies of tree-dwelling frogs, and the first use of eDNA for amphibian monitoring in Madagascar. This pilot study expands on a previously developed method and aims to provide a simple field protocol for DNA extraction from very small water samples, using a relatively inexpensive kit compared to other collection methods. We collected 20 ml of water from tree holes in Ambohitantely Special Reserve in Madagascar, with the aim to survey for the Critically Endangered tree frog Anodonthyla vallani, and we developed species specific cytochrome c oxidase 1 primers for this species. While our two samples did not detect A. vallani, we successfully extracted up to 16.6 ng/µl of eDNA from the samples and using 16S rRNA primers barcoded the tree frog Plethodontohyla mihanika in one of the samples. Despite just two samples being collected, we highlight the future potential of eDNA from tree holes for investigating cryptic habitat specialist amphibians given we extracted frog eDNA from just 20 ml of water. The method provides a rapid, simple, and cost-effective method which can assist cryptic species monitoring in challenging and time-consuming field conditions and should be developed further for frog surveying in Madagascar and beyond. The newly developed primers can be used for further work using this eDNA method to survey threatened Anodonthyla frog species.

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