The Hirtodrosophila melanderi species group (Diptera: Drosophilidae) from the Huanglong National Nature Reserve, Sichuan, China

Zootaxa ◽  
2019 ◽  
Vol 4623 (1) ◽  
pp. 113-131
Author(s):  
YU MEI ◽  
TAKEHIRO K. KATOH ◽  
JIAN-JUN GAO
Keyword(s):  
Species Delimitation ◽  
Nature Reserve ◽  
Field Survey ◽  
Species Group ◽  
Tibet Plateau ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
National Nature Reserve ◽  
Qinghai Tibet Plateau ◽  
I Gene

The Hirtodrosophila melanderi species group is currently known for thirteen described species, most of which were thought to be fungivorous. More than half known species of this species group were recorded exclusively from high altitude zone to the southeast of the Qinghai-Tibet Plateau in China. In our recent field survey in the Huanglong National Nature Reserve (located to the east of the Qinghai-Tibet Plateau) in Sichuan Province, China, we collected dozens of specimens of the H. melanderi group there. In the present study, these specimens are subjected to species delimitation based on data of not only morphology, but also DNA barcodes (nucleotide sequences of a 658-bp fragment of the mitochondrial cytochrome c oxidase subunit I gene). The five new species thus recognized are described: Hirtodrosophila minshanensis sp. nov., H. lambda sp. nov., H. zhangae sp. nov., H. zouae sp. nov., and H. nigrispina sp. nov. In addition, an updated key to all species of the H. melanderi species group is provided. 

Morphological and molecular evidence for a new species of the genus Panolis Hübner, [1821] (Lepidoptera: Noctuidae)

Zootaxa ◽  
2019 ◽  
Vol 4565 (4) ◽  
pp. 523
Author(s):  
XUAN AN ◽  
MAMORU OWADA ◽  
MIN WANG ◽  
HOU-SHUAI WANG
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Molecular Evidence ◽  
Mitochondrial Cytochrome ◽  
Molecular Phylogenetic ◽  
Lepidoptera Noctuidae ◽  
A New Species ◽  
I Gene

A new species of the genus Panolis Hübner, [1821], P. xundian sp. nov., is described and illustrated from southwestern China. It is well-defined morphologically by the male genitalia, with a well-developed pollex at the distal terminal of the sacculus and a broad, ventrally concave cucullus, the female corpus bursae with four long signum-stripes. Based on a 658 bp segment of the mitochondrial cytochrome c oxidase subunit I gene, we report the pairwise genetic distance of 2.5% from its allied species P. exquisita Draudt, 1950. Molecular phylogenetic analyses using three genes (2189 bp in total length) indicate that the new species belongs to the P. exquisita species group. 

Exploring the diversity of Asian Cryptocercus (Blattodea : Cryptocercidae): species delimitation based on chromosome numbers, morphology and molecular analysis

2018 ◽  
Vol 32 (1) ◽  
pp. 69 ◽  
Author(s):  
Qikun Bai ◽  
Lili Wang ◽  
Zongqing Wang ◽  
Nathan Lo ◽  
Yanli Che
Keyword(s):  
Chromosome Number ◽  
Species Delimitation ◽  
Phylogenetic Analyses ◽  
Western China ◽  
Tibet Plateau ◽  
28S Rrna ◽  
Candidate Species ◽  
The Rich ◽  
Coi Sequences ◽  
Qinghai Tibet Plateau

Woodroaches from the genus Cryptocercus Scudder, 1862 are known to display low levels of morphological divergence, yet significant genetic divergence and variability in chromosome number. Compared with Cryptocercus taxa from North America, the diversity of the genus in Asia has received relatively little attention. We performed morphological and karyotypic examinations of multiple taxa from several previously unsampled mountainous areas of central and south-western China, and identified nine candidate species primarily on the basis of chromosome number. We then investigated diversity across all Asian Cryptocercus, through phylogenetic analyses of 135 COI sequences and 74 28S rRNA sequences from individuals of 28 localities, including species delimitation analysis in General Mixed Yule Coalescent (GMYC) and Automatic Barcode Gap Discovery (ABGD). Phylogenetic results indicated that individuals from the same locality constituted well supported clades. The congruence of GMYC and ABGD results were in almost perfect accord, with 28 candidate species described on the basis of karyotypes (including the nine identified in this study). We provide evidence that each valley population in the Hengduan Mountains contains a separate evolving lineage. We conclude that the principal cause of the rich Cryptocercus diversity in China has been the uplift of the Qinghai-Tibet Plateau.

scholarly journals Revision of the species of Lytopylus from Area de Conservación Guanacaste, northwestern Costa Rica (Hymenoptera, Braconidae, Agathidinae)

ZooKeys ◽  
2017 ◽  
Vol 721 ◽  
pp. 93-158 ◽  
Author(s):  
Ilgoo Kang ◽  
Eric G. Chapman ◽  
Daniel H. Janzen ◽  
Winnie Hallwachs ◽  
Tanya Dapkey ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Dna Barcode ◽  
Mitochondrial Cytochrome ◽  
Neighbor Joining ◽  
Ecological Information ◽  
Dichotomous Key ◽  
Two New Species ◽  
Gene Coi ◽  
Collection Date ◽  
I Gene

Thirty two new species of Lytopylus (Agathidinae) are described with image plates for each species: Lytopylus alejandromasisisp. n., Lytopylus alfredomainierisp. n., Lytopylus anamariamongeaesp. n., Lytopylus angelagonzalezaesp. n., Lytopylus cesarmoraisp. n., Lytopylus eddysanchezisp. n., Lytopylus eliethcantillanoaesp. n., Lytopylus ericchapmanisp. n., Lytopylus gahyunaesp. n., Lytopylus gisukaesp. n., Lytopylus guillermopereiraisp. n., Lytopylus gustavoinduniisp. n., Lytopylus hartmanguidoisp. n., Lytopylus hernanbravoisp. n., Lytopylus hokwonisp. n., Lytopylus ivanniasandovalaesp. n., Lytopylus johanvalerioisp. n., Lytopylus josecortesisp. n., Lytopylus luisgaritaisp. n., Lytopylus mariamartachavarriaesp. n., Lytopylus miguelviquezisp. n., Lytopylus motohasegawaisp. n., Lytopylus okchunaesp. n., Lytopylus pablocobbisp. n., Lytopylus robertofernandezisp. n., Lytopylus rogerblancoisp. n., Lytopylus salvadorlopezisp. n., Lytopylus sangyeonisp. n., Lytopylus sarahmeierottoaesp. n., Lytopylus sergiobermudezisp. n., Lytopylus sigifredomarinisp. n., and Lytopylus youngcheaesp. n. A dichotomous key and a link to an electronic, interactive key are included. All specimens were reared from Lepidoptera larvae collected in Area de Conservación Guanacaste (ACG) and all are associated with ecological information including host caterpillar, collection date, eclosion date, caterpillar food plant, and locality. Neighbor-joining and maximum likelihood analyses of the barcode region of the mitochondrial cytochrome c oxidase subunit I gene (COI DNA barcode) were conducted to aid in species delimitation.

Revision of the subgenus Phortica (sensu stricto) (Diptera, Drosophilidae) from East Asia, with assessment of species delimitation using DNA barcodes

Zootaxa ◽  
2019 ◽  
Vol 4678 (1) ◽  
pp. 1-75
Author(s):  
JIA HUANG ◽  
LU GONG ◽  
SHUN-CHERN TSAUR ◽  
LIN ZHU ◽  
KEYING AN ◽  
...  
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Species Delimitation ◽  
Species Complex ◽  
Dna Barcode ◽  
Sensu Stricto ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Dna Barcodes ◽  
New Synonyms

A total of 50 (43 known and seven new) species in the subgenus Phortica (sensu stricto) were surveyed and (re)described from China: P. bicornuta (Chen & Toda, 1997); P. bipartita (Toda & Peng, 1992); P. biprotrusa (Chen & Toda, 1998); P. cardua (Okada, 1977); P. chi (Toda & Sidorenko, 1996); P. conifera (Okada, 1977); P. eparmata (Okada, 1977); P. eugamma (Toda & Peng, 1990); P. excrescentiosa (Toda & Peng, 1990); P. fangae (Máca, 1993); P. flexuosa (Zhang & Gan, 1986); P. foliata (Chen & Toda, 1997); P. gamma (Toda & Peng, 1990); P. gigas (Okada, 1977); P. glabtabula Chen & Gao, 2005; P. hainanensis (Chen & Toda, 1998); P. hongae (Máca, 1993); P. huazhii Cheng & Chen, 2008; P. iota (Toda & Sidorenko, 1996); P. jadete Zhu, Cao & Chen, 2018; P. kappa (Máca, 1977); P. lambda (Toda & Peng, 1990); P. latifoliacea Chen & Watabe, 2008; P. magna (Okada, 1960); P. okadai (Máca, 1977); P. omega (Okada, 1977); P. orientalis (Hendel, 1914); P. pangi Chen & Wen, 2005; P. paramagna (Okada, 1971); P. perforcipata (Máca & Lin, 1993); P. pi (Toda & Peng, 1990); P. protrusa (Zhang & Shi, 1997); P. pseudopi (Toda & Peng, 1990); P. pseudotau (Toda & Peng, 1990); P. psi (Zhang & Gan, 1986); P. rhagolobos Chen & Gao, 2008; P. saeta (Zhang & Gan, 1986); P. setitabula Chen & Gao, 2005; P. subradiata (Okada, 1977); P. tau (Toda & Peng, 1990); P. uncinata Chen & Gao, 2005; P. unipetala Chen & Wen, 2005; P. allomega Gong & Chen, sp. nov.; P. archikappa Gong & Chen, sp. nov.; P. dianzangensis Gong & Chen, sp. nov.; P. imbacilia Gong & Chen, sp. nov.; P. liukuni Gong & Chen, sp. nov.; P. tibeta Gong & Chen, sp. nov.; and P. xianfui Gong & Chen, sp. nov. In addition, seven new synonyms were recognized: P. acongruens (Zhang & Shi, 1997), syn. nov.; P. antillaria (Chen & Toda, 1997), syn. nov.; P. kukuanensis Máca, 2003, syn. nov.; P. linae (Máca & Chen, 1993), syn. nov.; P. shillongensis (Singh & Gupta, 1979), syn. nov.; P. takadai (Okada, 1977), syn. nov.; and P. watanabei (Máca & Lin, 1993), syn. nov. A key to all Asian species (except for the eparmata species complex) of this subgenus was provided. All currently available DNA barcode (partial mitochondrial cytochrome c oxidase subunit I (COI) gene) sequences of this subgenus (217 sequences of 54 species) are employed in a molecular analysis using different species delimitation methods. The results indicate that approximately 68.5% (37 of 54 spp.) of Phortica (s. str.) species could be clearly distinguished from closely related morphospecies or cryptic species. 

scholarly journals Spatial Origin and Diversification of the Lycocerus fainanus Species Group (Coleoptera, Cantharidae), with Descriptions of Four New Species from China and Vietnam

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 445
Author(s):  
Huacong Xi ◽  
Younan Wang ◽  
Tong Liu ◽  
Xingke Yang ◽  
Haoyu Liu ◽  
...  
Keyword(s):  
New Species ◽  
Southwest China ◽  
Mainland China ◽  
Molecular Data ◽  
Species Group ◽  
Morphological Data ◽  
Tibet Plateau ◽  
Distribution Map ◽  
Mcmc Method ◽  
Qinghai Tibet Plateau

Five previously known species were attributed to the Lycocerus fainanus species group, including L. inopaciceps (Pic 1926), L. oberthueri (Gorham 1889), L. oudai (Švihla 2004), L. metallipennis (Fairmaire 1887), and L. nigripes (Wittmer, 1995). Four new species of this group were discovered from China and Vietnam, L. binotatus sp. nov., L. testacicollis sp. nov., L. daliensis sp. nov., and L. vietnamensis sp. nov. An updated key to all species was provided. A geographical distribution map is presented, which shows that all the members were located between 18.69041–33.93441° N, and between 98.61413–121.77102° E. The ancestral geographical range was reconstructed based on a phylogeny of morphological data by the Bayesian Binary MCMC method. The result showed that the spatial origin of L. fainanus species group was probably located in northern Vietnam and southwest China. The divergence of the species in southwest China and Taiwan was caused by vicariance about 24 Ma ago, when the latter was separated in the Qinghai-Tibet Plateau, and the remaining species of mainland China all originated from Taiwan after traveling around Southeast Asia and back to China. Nevertheless, this conclusion should be verified when fossil evidence and molecular data are available.

scholarly journals Two new species of Lithobius on Qinghai-Tibetan plateau identified from morphology and COI sequences (Lithobiomorpha: Lithobiidae)

ZooKeys ◽  
2018 ◽  
Vol 785 ◽  
pp. 11-28 ◽  
Author(s):  
Penghai Qiao ◽  
Wen Qin ◽  
Huiqin Ma ◽  
Tongzuo Zhang ◽  
Jianping Su ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Molecular Data ◽  
Tibet Plateau ◽  
Mitochondrial Cytochrome ◽  
Distance Determination ◽  
Two New Species ◽  
Molecular Phylogenetic ◽  
Coi Sequences ◽  
Qinghai Tibet Plateau

Lithobius (Ezembius) longibasitarsussp. n. and Lithobius (Ezembius) datongensissp. n. (Lithobiomorpha: Lithobiidae), recently discovered from Qinghai-Tibet Plateau, China, are described. A key to the species of the subgenus Ezembius in China is presented. The partial mitochondrial cytochrome c oxidase subunit I barcoding gene was amplified and sequenced for eight individuals of the two new species and the dataset was used for molecular phylogenetic analysis and genetic distance determination. Both morphology and molecular data show that the specimens examined should be referred to Lithobius (Ezembius).

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