scholarly journals Laboratory Synthesis and Titrations of IronIIchloride-FeCl<sub>2</sub> and IronII Ethoxide-Fe(OEt)<sub>2</sub> Using Ethanol-90° - Hexane and Sodium Ethoxide-Na(OEt)

2021 ◽  
Vol 9 (5) ◽  
pp. 123
Author(s):  
Nambinina Richard Randriana ◽  
Avotra Marson Randrianomenjanahary ◽  
Andry Tahina Rabeharitsara
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.


2017 ◽  
Vol 51 (4) ◽  
pp. 315-328
Author(s):  
Arturo Mendoza-Flores ◽  
Mario Villalobos ◽  
Teresa Pi-Puig ◽  
Nadia Valentina Martínez-Villegas

1927 ◽  
Vol 0 (0) ◽  
pp. 194-200 ◽  
Author(s):  
George Macdonald Bennett ◽  
Leslie Vivian Donald Scorah
Keyword(s):  

1967 ◽  
Vol 0 (0) ◽  
pp. 1103-1108 ◽  
Author(s):  
P. Beltrame ◽  
P. L. Beltrame ◽  
O. Sighinolfi ◽  
M. Simonetta
Keyword(s):  

Author(s):  
Joshua Hollingshead ◽  
Makayla L. Ianuzzi ◽  
Jeffrey D. Moore ◽  
Grant A. Risha

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