scholarly journals Frequency of Deletion of AZF Region of the Y Chromosome in Chhattishgarh, India

2021 ◽  
Vol 9 (2) ◽  
pp. 36
Author(s):  
Manisha Barnwal Sinha ◽  
Rima Dada ◽  
Suprava Patel ◽  
Apoorva Joshi ◽  
Nilaj Bagde ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Azf Region

scholarly journals The role of modern technologies in the management of azoospermia using microdissection TESE in the framework of the ECF-ICSI program. A literature review. Part I.

2012 ◽  
Vol 58 (5) ◽  
pp. 66-74
Author(s):  
I I Vitiazeva ◽  
S V Bogoliubov ◽  
I I Dedov
Keyword(s):  
Prognostic Factors ◽  
Literature Review ◽  
Male Infertility ◽  
Y Chromosome ◽  
Modern Methods ◽  
Modern Classification ◽  
Modern Technologies ◽  
Azf Region

The present review is focused on the modern methods for the treatment of male infertility related to obstructive and unobstructive azoospermia. The criteria and prognostic factors for obtaining spermatozoa by means of invasive manipulations on the testicles are considered. Special attention is given to the results of investigations into the state of the AZF-region of Y-chromosome and of the studies of patients presenting with Klinefelter's syndrome. The modern classification of testicular biopsies is presented that allows to discriminate between different forms of compromised spermatogenesis.

scholarly journals The frequency of terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome

1970 ◽  
Vol 21 ◽  
pp. 301-305
Author(s):  
I. Ye. Haiboniuk ◽  
M. Ya. Tyrkus ◽  
H. V. Makukh
Keyword(s):  
Y Chromosome ◽  
Molecular Genetic ◽  
Terminal Deletion ◽  
Molecular Genetic Study ◽  
Salting Out ◽  
Azfc Region ◽  
Specific Pcr ◽  
Allele Specific ◽  
Keywords Male Infertility ◽  
Azf Region

Aim. Determine the frequency of absence marker terminal deletions sY160 among men with microdeletions of AZFc region Y-chromosome. Methods. DNA from probands blood samples was isolated using a modified salting out method. Microdeletions of Y chromosome AZF region were analyzed using two multiplex PCR. The molecular-genetic study of terminal deletions (absence of sY160) was carried out using allele-specific PCR and analysed by electrophoresis in a 2 % agarose gel. Results. Among infertile men (1500 individuals), Y chromosome microdeletions were detected in 7% males: microdeletions of AZFa subregion in 1 %, AZFb subregion – 3 %, AZF(b+c) subregions – 15 %, AZFc subregion – 67 %. The presence of heterochromatin marker sY160 was confirmed in 39 cases (84.8 %) and absence of sY160 in 7 men (15.2 %). Absence of sY160 was detected in 2 men with AZFc microdeletion and in 5 men with AZFb+AZFc microdeletions. It is important to point out that terminal AZFc deletion was confirmed in 83.3 % of cases of AZFb+c microdeletions and only in 5.1 % of isolated AZFc microdeletions. Conclusions. Thus, among 15.2 % man with different AZF microdeletions of Y-chromosome the heterochromatin marker of terminal deletion sY160 was absents. The implementation of testing of marker of terminal deletion – sY160 may help to determine if the deletion corresponds to the b2/b4 pattern and to avoid biopsy in man which most likely not benefit from the surgical procedure. Keywords: male infertility, spermatogenesis, Y chromosome, AZF region, terminal deletions.

Prognostic factors for the success of obtaining spermatozoa with a biopsy of testicular tissue in men with severe forms of pathozoospermia

2020 ◽  
pp. 96-97
Author(s):  
Т.А. Яманди ◽  
Н.Ю. Сафина ◽  
В.Б. Черных ◽  
Л.В. Акуленко
Keyword(s):  
Prognostic Factors ◽  
In Vitro Fertilization ◽  
Y Chromosome ◽  
Testicular Biopsy ◽  
Testicular Tissue ◽  
Inhibin B ◽  
Severe Oligozoospermia ◽  
Vitro Fertilization ◽  
Azf Region

Приводятся данные обследования мужчин с азооспермией и олигозооспермией тяжелой степени, имеющих и не имеющих микроделеции длинного плеча Y-хромосомы, а также результаты биопсии тестикулярной ткани. Отсутствие делеций региона AZF, отсутствие гипоплазии яичек, а также нормальные показатели ФСГ, ЛГ и ингибина В являются прогностически благоприятными критериями в отношении успешности получения сперматозоидов при биопсии ткани яичка для проведения экстракорпорального оплодотворения (ЭКО). The article presents the results of a survey of men with azoospermia and severe oligozoospermia, with and without microdeletion of the long arm of the Y chromosome, as well as the results of a testicular biopsy. The absence of deletions of the AZF region, the absence of testicular hypoplasia, as well as normal levels of FSH, LH and inhibin B are prognostically favorable criteria for the success of obtaining spermatozoa with a biopsy of testicular tissue for in vitro fertilization (IVF).

Copy number variants within AZF region of Y chromosome and their association with idiopathic male infertility in Serbian population

Andrologia ◽  
2021 ◽  
Author(s):  
Nemanja Vučić ◽  
Nevena Kotarac ◽  
Suzana Matijašević ◽  
Lana Radenković ◽  
Ivan Vuković ◽  
...  
Keyword(s):  
Male Infertility ◽  
Y Chromosome ◽  
Copy Number ◽  
Copy Number Variants ◽  
Idiopathic Male Infertility ◽  
Azf Region

Do microdeletions in the AZF region of the Y chromosome accompany cryptorchidism in Turkish children?

2007 ◽  
Vol 40 (3) ◽  
pp. 577-581 ◽  
Author(s):  
Necati Gurbuz ◽  
Bedi Ozbay ◽  
Bekir Aras ◽  
Ali Ihsan Tasci
Keyword(s):  
Y Chromosome ◽  
Turkish Children ◽  
Azf Region

Clinical, Hormonal, and Genetic Evaluation of Idiopathic Nonobstructive Azoospermia and Klinefelter Syndrome Patients

2017 ◽  
Vol 153 (4) ◽  
pp. 190-197 ◽  
Author(s):  
Shin Y. Kim ◽  
Bom Y. Lee ◽  
Ah R. Oh ◽  
So Y. Park ◽  
Hyo S. Lee ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Klinefelter Syndrome ◽  
Nonobstructive Azoospermia ◽  
Specific Sequence ◽  
Azoospermia Factor ◽  
Sequence Tagged Sites ◽  
Infertile Men ◽  
Y Chromosome Microdeletions ◽  
Genetic Abnormalities ◽  
Azf Region

To investigate the clinical, hormonal, and genetic factors in infertile men with idiopathic nonobstructive azoospermia (NOA) or azoospermic Klinefelter syndrome (KFS), a total of 556 and 96 patients, respectively, were included in this study. All patient samples were analyzed cytogenetically. Serum reproductive hormone levels were measured. Microdeletions in the azoospermia factor (AZF) region of the Y chromosome were detected by multiplex PCR using 16 specific sequence-tagged sites. FSH and LH levels in both NOA and KFS patients were significantly higher than the normal range, and the testosterone level in KFS patients was significantly lower. Ninety-two (95.8%) of the KFS patients showed non-mosaic 47,XXY karyotypes and 47,XXY,inv(9)(p11.1q13); the other KFS patients had mosaic karyotypes of 47,XXY/46,XY, 47,XXY/46,XX, and 47,XXY/48,XXXY/46,XX. Among the 556 idiopathic NOA patients with normal karyotypes, 67 (12.05%) had microdeletions in the AZF region of the Y chromosome. Microdeletions were most frequently detected in the AZFc region, followed by AZFa, AZFb, AZFbc, and partial AZFc deletions. However, Y chromosome microdeletions were not found in any of the azoospermic KFS patients. In view of the hormonal and genetic abnormalities in infertile men with idiopathic NOA and with azoospermic KFS, genetic testing for karyotype, Y chromosome microdeletions, and hormonal parameters is advocated.

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