scholarly journals An Antisense of Protein Kinase C- ζ Inhibits Proliferation of Human Airway Smooth Muscle Cells

2000 ◽  
Vol 23 (4) ◽  
pp. 555-559 ◽  
Author(s):  
Stephen Carlin ◽  
Philip Poronnik ◽  
David I. Cook ◽  
Lee Carpenter ◽  
Trevor J. Biden ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Human Airway Smooth Muscle ◽  
Human Airway ◽  
Kinase C

Protein kinase C‐ε mediates bradykinin‐induced cyclooxygenase‐2 expression in human airway smooth muscle cells

2002 ◽  
Vol 16 (11) ◽  
pp. 1435-1437 ◽  
Author(s):  
Linhua Pang ◽  
Mei Nie ◽  
Lisa Corbett ◽  
Richard Donnelly ◽  
Samuel Gray ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Human Airway Smooth Muscle ◽  
Human Airway ◽  
Kinase C

scholarly journals Phosphorylation of Kv7 potassium channels by protein kinase C in human airway smooth muscle cells

2013 ◽  
Vol 27 (S1) ◽  
Author(s):  
Jennifer M Haick ◽  
Lioubov I Brueggemann ◽  
Jeffrey R Prochot ◽  
Devjit S Randhawa ◽  
Christopher Wigfield ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Potassium Channels ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Human Airway Smooth Muscle ◽  
Kinase C

scholarly journals Protein Kinase C-ε Regulates Local Calcium Signaling in Airway Smooth Muscle Cells

2009 ◽  
Vol 40 (6) ◽  
pp. 663-671 ◽  
Author(s):  
Qing-Hua Liu ◽  
Yun-Min Zheng ◽  
Amit S. Korde ◽  
Xiao-Qiang Li ◽  
Jianjie Ma ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Smooth Muscle Cells ◽  
Calcium Signaling ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Kinase C

Protein kinase C isoforms in human airway smooth muscle cells: activation of PKC-ζ during proliferation

1999 ◽  
Vol 276 (3) ◽  
pp. L506-L512 ◽  
Author(s):  
Steve Carlin ◽  
Kenny X. F. Yang ◽  
Richard Donnelly ◽  
Judith L. Black
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Western Blot ◽  
Smooth Muscle Cells ◽  
Airway Smooth Muscle ◽  
Muscle Cells ◽  
Airway Smooth Muscle Cells ◽  
Kinase C ◽  
Pkc Ζ

Protein kinase C (PKC) is implicated in the regulation of smooth muscle contractility and growth. We have previously described the pattern of isoform expression of PKC in canine airway smooth muscle. This study identified the isoforms present in human cultured airway smooth muscle cells and also addressed the question of whether mitogenesis in these cells is associated with changes in a specific isoform, PKC-ζ. Western blot analysis revealed the presence of PKC-α, -βI, and -βII of the conventional group; PKC-δ, -θ, -ε, and -η of the novel group; and PKC-ζ, -μ, and -ι of the atypical group. There was a significant increase in density of the Western blot for PKC-ζ in cells proliferating in response to 10% fetal bovine serum (FBS) to 372 ± 115% of control values ( P < 0.05; n = 3 patients) in the cytosolic fraction. Platelet-derived growth factor (PDGF) produced increases in PKC-ζ in both the cytosolic and membrane fractions to 210 ± 49 and 443 ± 227%, respectively, of control values ( P < 0.05; n = 4 patients). There was no change in expression of PKC-α, -βI, -βII, -θ, -ε, -η, -δ, or -ι in response to the same stimuli. PGE2 (1 μM) added to the cells 30 min before PDGF reduced incorporation of [3H]thymidine from 5,580 ± 633 (SE) to 3,980 ± 126 dpm ( P < 0.05; n = 3 patients) and, in addition, reduced expression of PKC-ζ in the membrane fraction as determined by Western blotting from 266 ± 66 to 110 ± 4% of control values ( P < 0.05; n = 3 patients). PKC-ζ activity in stimulated cells (10% FBS), as assessed by immunoprecipitation and phosphorylation of glycogen synthase peptide, was ∼3-fold greater than that in unstimulated cells, and the amount of PKC-ζ protein correlated with isoenzyme activity ( r 2 = 0.91; P < 0.02; n = 4 patients). In conclusion, this study 1) provides the first description of which isoforms of PKC are present in human cultured airway smooth muscle cells and 2) shows that proliferation of these cells is associated with upregulation of PKC-ζ. Whether activation of PKC-ζ is a primary or secondary event in airway smooth muscle cell proliferation remains to be determined.

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