Study on the Establishment of a Rapid Propagation System for Succulent Plant Haworthia heidelbergensis

2020 ◽  
Vol 14 (5) ◽  
pp. 632-638
Author(s):  
Zhongyong Cen ◽  
Jiang Su ◽  
Hongrun Tu ◽  
Shiting Lin
Keyword(s):  
In Vitro Culture ◽  
Callus Induction ◽  
Proliferation Rate ◽  
Medium Component ◽  
Succulent Plant ◽  
Induction Rate ◽  
Rapid Propagation ◽  
Callus Differentiation ◽  
Optimal Medium

This study took the inflorescence and leaves of the succulent plant Haworthia heidelbergensis as explants, and explored the effects of different mediums with different hormone ratios on the rapid propagation of Haworthia heidelbergensis. The results showed that the optimal medium component for inflorescence callus induction was MS (Murashige and Skoog)+2.0 mg/L 6-BA+1.0 mg/L 2,4-D+0.2 mg/L NAA, the callus induction rate was 90%; the optimal medium component for leaf callus induction was MS+0.5 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA; the optimal medium for callus differentiation was MS+1.0 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA, the clump bud differentiation rate was 50%; the optimal medium for clump bud proliferation was MS+0.5 mg/L 6-BA+1.0 mg/L KT+0.05 mg/L NAA, the proliferation rate was 600%; the best medium for rooting was 1/2 MS+ 0.2 mg/L NAA+0.3 g/L AC. In conclusion, this study selected the explants and culture mediums, established an aseptic propagation system and provided a reference for the in vitro culture and rapid propagation of Haworthia heidelbergensis.

scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

scholarly journals In Vitro Culture of Heuchera villosa ‘Caramel’

HortScience ◽  
2017 ◽  
Vol 52 (4) ◽  
pp. 622-624 ◽  
Author(s):  
Hua Q. Zhao ◽  
Qing H. He ◽  
Li L. Song ◽  
Mei F. Hou ◽  
Zhi G. Zhang
Keyword(s):  
Acetic Acid ◽  
In Vitro Culture ◽  
Butyric Acid ◽  
Shoot Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Induction Rate ◽  
Regenerated Plantlets ◽  
Indole 3 Acetic Acid

The procedure for Heuchera villosa ‘Caramel’ propagation was investigated, which involves shoot regeneration, rooting of regenerated shoots, and acclimation of regenerated plantlets. Petioles, as explants, were cultured on MS medium supplemented with 1-naphthylacetic acid (NAA), benzylaminopurine (BA), thidiazuron (TDZ) and callus formed on all media. Shoots were observed to proliferate from callus on media with BA and NAA, whereas no shoots regenerated on media with TDZ and NAA. On media containing 0.5 or 1.0 mg·L−1 BA in combination with NAA, the regenerated shoots showed severe hyperhydricity, whereas on media containing 0.1 mg·L−1 BA in combination with NAA, the regenerated shoots grew normally. The highest shoot induction rate, 90.6%, was obtained on media containing 0.1 mg·L−1 BA and 0.01 mg·L−1 NAA. The effects of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), and NAA on rooting of H. villosa ‘Caramel’ was explored. The highest rooting rate (95%) was obtained on 1/2 MS medium containing 0.2 mg·L−1 NAA. In the subsequent acclimation experiments, about 85% of rooted plantlets survived and grew normally.

In vitro culture and rapid propagation of Ilex asprella

2020 ◽  
Vol 12 (1) ◽  
pp. 114
Author(s):  
Xu Shiqiang ◽  
Ying Xiaobao ◽  
Cai Shike ◽  
Mei Yu ◽  
Wang Jihua
Keyword(s):  
In Vitro Culture ◽  
Rapid Propagation

scholarly journals In Vitro Indirect Plantlet Regeneration from Hypocotyl Segments and Cotyledonary Explant Derived Calli in Lady's Finger (Abelmoschus esculentus L. Monech)

1970 ◽  
Vol 16 ◽  
pp. 49-57
Author(s):  
MT Rahman ◽  
MJ Hossain ◽  
M Khalekuzzaman
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Abelmoschus Esculentus ◽  
Root Induction ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Induction Rate ◽  
Cotyledonary Explants ◽  
Hypocotyl Segments

An experiment was conducted to develop an efficient protocol for in vitro regeneration of plantlets from hypocotyl segments and cotyledonary explants of Abelmoschus esculentus L. Monech. In this investigation the cultivar OK-285 of Abelmoschus esculentus L. Monech was used. At first somatic embryogenic calli were induced using MS medium supplemented with different concentrations of auxin and cytokinin singly or in combination. The cotyledonary explants showed the best callus induction rate (87.9%) in MS medium containing 1.5mgl-l 2.4-D + 0.1mgl-l BAP hormonal concentration while hypocotyl segments showed 82.6% callus induction rate in the same medium. For shoot formation, calli were subcultured on MS solid medium and the hypocotyl segments showed the best result (72.1%) in MS medium containing 2.0 mgl-l BAP + 0.1 mgl-l IAA and the mean number of shoots per callus was recorded 4.2. For root induction from shoots MS and ½MS media were used. The highest 63.5% microshoots initiated roots in ½ MS + 0.1 mgl-l IBA medium and the highest mean number of root was 4.8. Rooted shoots were acclimated and successfully established into soil under natural condition with 70% survival. Key words: Abelmoschus esculentus, hypocotyl segments and cotyledon, embryogenic calli, regeneration.   DOI:10.3329/jbs.v16i0.3741 J. bio-sci. 16: 49-57, 2008

scholarly journals Development of Optimal Medium Content for Bioelements Accumulation in Bacopa monnieri (L.) In Vitro Culture

2014 ◽  
Vol 174 (4) ◽  
pp. 1535-1547 ◽  
Author(s):  
Maciej Łojewski ◽  
Bożena Muszyńska ◽  
Agata Smalec ◽  
Witold Reczyński ◽  
Włodzimierz Opoka ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Bacopa Monnieri ◽  
Optimal Medium

In vitro culture of coconut endosperm: callus induction and its fatty acids

1992 ◽  
Vol 11 (11) ◽  
Author(s):  
MyrnaS. Ceniza ◽  
Shinta Ueda ◽  
Yukio Sugimura
Keyword(s):  
Fatty Acids ◽  
In Vitro Culture ◽  
Callus Induction
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