Effect of Jasmonic Acid (JA) and Glutamine on Callus Induction of Madagascar Periwinkle Plant (Catharanthus roseus L. cv. Nirvana Pink Blush) by in vitro Culture

An experiment on the effect of sucrose and mannitol on leave induced callus of Catharanthus roseus was conducted from February 2011 to May 2012. Callus induction was achieved by culturing leaves of the plant on MS medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin, The best medium to maintain callus was on MS medium modified with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. when different levels were added to MS medium for each Mannitol 0, 6000 ,8000 ,10000 mg /L and Sucrose 40, 60 ,80, 100 g /L in split experimental and control treatment was MS medium supplemented with 30 g/L sucrose. The results showed that medium supplemented with 100 gL of sucrose gave the highest quantity of Ajmalicine 32.27 µg/100 mg fresh weight of callus,as well as medium supplemented with 8000 mgL of Mannitol gave the highest value of Ajmalicine 120.19 µg/100 mg fresh weight of callus. The concentrations of Ajmalicine, derived from the leaves of the plants grown in pot, were lowest than the concentrations produced by the callus grown in vitro it was 0.047 µg/100 fresh weight of the leaves.

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Study on the Establishment of a Rapid Propagation System for Succulent Plant Haworthia heidelbergensis

Journal of Biobased Materials and Bioenergy ◽

10.1166/jbmb.2020.2001 ◽

2020 ◽

Vol 14 (5) ◽

pp. 632-638

Author(s):

Zhongyong Cen ◽

Jiang Su ◽

Hongrun Tu ◽

Shiting Lin

Keyword(s):

In Vitro Culture ◽

Callus Induction ◽

Proliferation Rate ◽

Medium Component ◽

Succulent Plant ◽

Induction Rate ◽

Rapid Propagation ◽

Callus Differentiation ◽

Optimal Medium

This study took the inflorescence and leaves of the succulent plant Haworthia heidelbergensis as explants, and explored the effects of different mediums with different hormone ratios on the rapid propagation of Haworthia heidelbergensis. The results showed that the optimal medium component for inflorescence callus induction was MS (Murashige and Skoog)+2.0 mg/L 6-BA+1.0 mg/L 2,4-D+0.2 mg/L NAA, the callus induction rate was 90%; the optimal medium component for leaf callus induction was MS+0.5 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA; the optimal medium for callus differentiation was MS+1.0 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA, the clump bud differentiation rate was 50%; the optimal medium for clump bud proliferation was MS+0.5 mg/L 6-BA+1.0 mg/L KT+0.05 mg/L NAA, the proliferation rate was 600%; the best medium for rooting was 1/2 MS+ 0.2 mg/L NAA+0.3 g/L AC. In conclusion, this study selected the explants and culture mediums, established an aseptic propagation system and provided a reference for the in vitro culture and rapid propagation of Haworthia heidelbergensis.

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Effect of gamma radiation on morphological and genetic variation in regenerated plantlets Catharanthus roseus L. (G) Don

Genetika ◽

10.2298/gensr2001015n ◽

2020 ◽

Vol 52 (1) ◽

pp. 15-28

Author(s):

Zahra Noormohammadi ◽

Narges Asghari-Mooneghi ◽

Farah Farahani

Keyword(s):

Gamma Radiation ◽

Catharanthus Roseus ◽

Genetic Difference ◽

Morphological Characteristics ◽

Stem Length ◽

Madagascar Periwinkle ◽

Genetic Changes ◽

Significant Difference ◽

Regenerated Plantlets

Catharanthus roseus L. (G) Don, commonly known as Madagascar periwinkle, is an important medicinal plant receiving much attention from researchers. This plant is a rich source of terpenoid indole alkaloids (TIAs). The gamma radiation and somaclonal variation may provide suitable methods for genetic changes to increase medicinal components of C. roseus. In the present study, 30 and 60 (Gy) gamma rays were irradiated on seeds. C. roseus seeds were cultured in Murashige and Skoog (MS) medium in three different groups, 0 Gy (control) 30, and 60 Gy gamma irradiations. Morphological characteristics of regenerated plantlets were measured for surface area of leaves, stem length, root length, and fresh weight. The genetic variations were evaluated using nine inter-simple sequence repeats and 25 sequences related amplified polymorphism markers. Morphological results showed a significant difference between studied groups in leaf surface and twisted leaf in 60 Gy irradiated plantlets. The analysis of molecular variance revealed the significant genetic difference among groups. STRUCTURE analysis showed genetic variability both within and among groups. Using the NJ tree analysis, the plants studied were categorized into three distinct groups, which were supported by principal coordinate analysis (PCoA). In conclusion, gamma irradiation produced morphological and genetic changes in C. roseus regenerated in vitro plantlets providing to be a suitable method for further mutation breeding in periwinkle.

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