scholarly journals Jacob’s ladder Polemonium caeruleum L. and black salsify Sсorzonera hispanica L. in vitro culture

2018 ◽  
Vol 22 ◽  
pp. 216-221
Author(s):  
O. V. Bulko ◽  
L. G. Lioshina
Keyword(s):  
In Vitro Culture ◽  
Root Culture ◽  
Medium Composition ◽  
Cultivation Medium ◽  
Plant Cultivation ◽  
Regenerated Plants ◽  
Composition Modification ◽  
Jacob's Ladder ◽  
Optimal Medium

Aim. Micropropagation of Jacob’s ladder Polemonium caeruleum L. and black salsify Scorzonera hispanica L., obtaining root culture and regenerated plants. Methods. In vitro plant cultivation, medium composition modification for micropropagation, inoculation of explants with agrobacterial strains. Results. In vitro cultures of Jacob’s ladder and black salsify have been obtained, the optimal medium composition has been determined for the effective plants multiplication, rooting and growth, root cultures and regenerated plants of studied species have been obtained. Conclusions. Obtained technology of in vitro culture establishment of P. caeruleum and S. hispanica can be used for plants microclonal propagation so as root culture and regenerated plants acquiring due to the agrobacterial transformation – for further studies of secondary metabolism of these plants. Keywords: P. caeruleum L., S. hispanica L., micropropagation, phytohormones, root culture.

In vitro culture of Aloe barbadensis Mill.: quantitative DNA variations in regenerated plants

Plant Science ◽  
1993 ◽  
Vol 91 (2) ◽  
pp. 223-229 ◽  
Author(s):  
A. Cavallini ◽  
L. Natali ◽  
G. Cionini ◽  
O. Sassoli ◽  
I. Castorena-Sanchez
Keyword(s):  
In Vitro Culture ◽  
Aloe Barbadensis ◽  
Regenerated Plants ◽  
Dna Variations

scholarly journals Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

2000 ◽  
Vol 23 (2) ◽  
pp. 479-493 ◽  
Author(s):  
Guillermo Pratta ◽  
Roxana Zorzoli ◽  
Liliana Amelia Picardi
Keyword(s):  
Multivariate Analysis ◽  
In Vitro Culture ◽  
Principal Component ◽  
Eigenvalues And Eigenvectors ◽  
Morphometric Traits ◽  
Regenerated Plants ◽  
Phenotypic Structure ◽  
Regeneration Cycle ◽  
The Stability

The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.

scholarly journals Regenerating Adventitious Shoots from in Vitro Culture of Liatris spicata (L.) Willd. Cotyledons

HortScience ◽  
1996 ◽  
Vol 31 (1) ◽  
pp. 154-155
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Ms Medium ◽  
Adventitious Shoot Formation ◽  
Regenerated Plants ◽  
Murashige And Skoog Medium ◽  
Homogeneous Chemical

Cotyledons from developing 6- to 8-week-old embryos of Liatris spicata (L.) Willd. (blazing star) were cultured on Murashige and Skoog medium containing 0, 0.4, 4.4, or 44.4 μm BA or 0, 0.2, 2.2, or 22.2 μm TDZ to induce adventitious shoot formation. The highest percentage of cotyledons forming the most shoots was on medium containing 2.2 μm TDZ. Cotyledon-derived callus cultured on medium containing 4.4 μm BA formed ≈16 times more adventitious shoots than on 2.2 μm TDZ. Adventitious shoots derived from cotyledons or callus produced roots when placed on MS medium containing 5.0 μm IBA. Regenerated plants that flowered in the field appeared homogeneous. Chemical names used: N6-benzyladenine (BA), thidiazuron (TDZ), indole-3-butyric acid (IBA).

THE IMPACT OF NUTRIENT MEDIUM COMPOSITION ON PROPAGATION OF PHLOX SIBIRICA L. IN IN VITRO CULTURE

2019 ◽  
Vol 0 (2) ◽  
pp. 66-72
Author(s):  
A.Sh. Akhmetova ◽  
◽  
A.A. Zaripova ◽  
A.I. Shigapova ◽  
◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Nutrient Medium ◽  
Medium Composition ◽  
The Impact

Study on the Establishment of a Rapid Propagation System for Succulent Plant Haworthia heidelbergensis

2020 ◽  
Vol 14 (5) ◽  
pp. 632-638
Author(s):  
Zhongyong Cen ◽  
Jiang Su ◽  
Hongrun Tu ◽  
Shiting Lin
Keyword(s):  
In Vitro Culture ◽  
Callus Induction ◽  
Proliferation Rate ◽  
Medium Component ◽  
Succulent Plant ◽  
Induction Rate ◽  
Rapid Propagation ◽  
Callus Differentiation ◽  
Optimal Medium

This study took the inflorescence and leaves of the succulent plant Haworthia heidelbergensis as explants, and explored the effects of different mediums with different hormone ratios on the rapid propagation of Haworthia heidelbergensis. The results showed that the optimal medium component for inflorescence callus induction was MS (Murashige and Skoog)+2.0 mg/L 6-BA+1.0 mg/L 2,4-D+0.2 mg/L NAA, the callus induction rate was 90%; the optimal medium component for leaf callus induction was MS+0.5 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA; the optimal medium for callus differentiation was MS+1.0 mg/L 6-BA+2.0 mg/L KT+0.3 mg/L NAA, the clump bud differentiation rate was 50%; the optimal medium for clump bud proliferation was MS+0.5 mg/L 6-BA+1.0 mg/L KT+0.05 mg/L NAA, the proliferation rate was 600%; the best medium for rooting was 1/2 MS+ 0.2 mg/L NAA+0.3 g/L AC. In conclusion, this study selected the explants and culture mediums, established an aseptic propagation system and provided a reference for the in vitro culture and rapid propagation of Haworthia heidelbergensis.

Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality

Zygote ◽  
2012 ◽  
Vol 21 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Rafael Rossetto ◽  
Márcia Viviane Alves Saraiva ◽  
Regiane Rodrigues dos Santos ◽  
Cleidson Manoel Gomes da Silva ◽  
Luciana Rocha Faustino ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Medium Composition ◽  
Minimum Essential Medium ◽  
Antral Follicles ◽  
Suitable Medium

SummaryThis study investigated the effect of three different culture media (α minimum essential medium (α-MEM), McCoy or TCM199 during the in vitro culture (IVC) of bovine isolated pre-antral follicles. Pre-antral follicles greater than 150 μm in size were isolated and cultured for 0 (control), 8 or 16 days in one of the abovementioned culture media. Follicles were evaluated for survival, growth and antrum formation at days 8 and 16. The results showed that TCM199 was the most suitable medium to preserve follicular viability and ultrastructure, resulting in the highest rates of antrum formation. In conclusion, TCM199 promotes the in vitro development of isolated pre-antral follicles without hampering follicular functionality by sustaining in vitro growth and antrum formation.

scholarly journals Development of Optimal Medium Content for Bioelements Accumulation in Bacopa monnieri (L.) In Vitro Culture

2014 ◽  
Vol 174 (4) ◽  
pp. 1535-1547 ◽  
Author(s):  
Maciej Łojewski ◽  
Bożena Muszyńska ◽  
Agata Smalec ◽  
Witold Reczyński ◽  
Włodzimierz Opoka ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Bacopa Monnieri ◽  
Optimal Medium

scholarly journals Biosynthesis of components with antifungal activity against Aspergillus spp. using Streptomyces hygroscopicus

2015 ◽  
Vol 69 (2) ◽  
pp. 201-208
Author(s):  
Jelena Dodic ◽  
Zorana Roncevic ◽  
Jovana Grahovac ◽  
Bojana Bajic ◽  
Olivera Korolija
Keyword(s):  
Antifungal Activity ◽  
Soybean Meal ◽  
Plant Protection ◽  
Desirability Function ◽  
Inhibition Zone ◽  
Medium Composition ◽  
Streptomyces Hygroscopicus ◽  
Medium Components ◽  
Optimal Medium

Losses of apple fruit during storage are mainly caused by fungal phytopathogens. Traditionally, postharvest fungal disease is controlled by the application of synthetic fungicides. However, the harmful impact on environment as well as human health largely limits their application. To reduce these problems in agrochemicals usage, new compounds for plant protection, which are eco-friendly, should be developed. The aim of this study is optimization of medium composition in terms of glucose, soybean meal and phosphates content, by applying response surface methodology, for the production of agents with antifungal activity against Aspergillus spp. For biosynthesis was used strain of Streptomyces hygroscopicus isolated from the environment. Experiments were carried out in accordance with Box-Behnken design with three factors on three levels and three repetitions in the central point. Antifungal activity of the obtained cultivation mediums against Aspergillus oryzae and Aspergillus niger was determined, in vitro, using the diffusion - disc method. For determination optimal medium components desirability function was used. Achieved model predicts that the maximum inhibition zone diameter (40.93 mm) against test microorganisms is produced when the initial content of glucose, soybean meal and phosphates are 47.77 g/l, 24.54 g/l and 0.98 g/l, respectively. To minimize the consumption of medium components and costs of effluents processing, additional three sets of optimization were made. The chosen method for optimization of medium components was efficient, relatively simple and time and material saving. Obtained results can be used for the further techno-economic analysis of the process to select optimal medium composition for industrial application.

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