Morphological Classification of Parvalbumin-Containing Retinal Ganglion Cells in Mouse: Single-Cell Injection after Immunocytochemistry

2006 ◽  
Vol 47 (7) ◽  
pp. 2757 ◽  
Author(s):  
Tae-Jin Kim ◽  
Chang-Jin Jeon
Keyword(s):  
Single Cell ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Morphological Classification ◽  
Cell Injection

A Morphological Classification of Retinal Ganglion Cells in the Japanese Catshark Scyliorhinus torazame

2014 ◽  
Vol 83 (3) ◽  
pp. 199-215 ◽  
Author(s):  
Kaori Muguruma ◽  
William K. Stell ◽  
Naoyuki Yamamoto
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Morphological Classification

Morphological classification of retinal ganglion cells in mice

1995 ◽  
Vol 356 (3) ◽  
pp. 368-386 ◽  
Author(s):  
Motoaki Doi ◽  
Yukitaka Uji ◽  
Hideki Yamamura
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Morphological Classification

Morphological classification of retinal ganglion cells in adultXenopus laevis

1988 ◽  
Vol 178 (2) ◽  
pp. 143-153 ◽  
Author(s):  
Charles Straznicky ◽  
Ivan T. Straznicky
Keyword(s):  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Retinal Ganglion ◽  
Morphological Classification

scholarly journals Author Correction: Single cell transcriptome profiling of retinal ganglion cells identifies cellular subtypes

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Bruce A. Rheaume ◽  
Amyeo Jereen ◽  
Mohan Bolisetty ◽  
Muhammad S. Sajid ◽  
Yue Yang ◽  
...  
Keyword(s):  
Single Cell ◽  
Retinal Ganglion Cells ◽  
Ganglion Cells ◽  
Transcriptome Profiling ◽  
Retinal Ganglion ◽  
Cell Transcriptome ◽  
Single Cell Transcriptome

scholarly journals Single Cell Sequencing of Induced Pluripotent Stem Cell Derived Retinal Ganglion Cells (iPSC-RGC) Reveals Distinct Molecular Signatures and RGC Subtypes

Genes ◽  
2021 ◽  
Vol 12 (12) ◽  
pp. 2015
Author(s):  
Harini V. Gudiseva ◽  
Vrathasha Vrathasha ◽  
Jie He ◽  
Devesh Bungatavula ◽  
Joan M. O’Brien ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Single Cell ◽  
Retinal Ganglion Cells ◽  
Pluripotent Stem Cell ◽  
Ganglion Cells ◽  
Induced Pluripotent Stem Cell ◽  
Retinal Ganglion ◽  
Single Cell Sequencing ◽  
Induced Pluripotent

We intend to identify marker genes with differential gene expression (DEG) and RGC subtypes in cultures of human-induced pluripotent stem cell (iPSC)-derived retinal ganglion cells. Single-cell sequencing was performed on mature and functional iPSC-RGCs at day 40 using Chromium Single Cell 3’ V3 protocols (10X Genomics). Sequencing libraries were run on Illumina Novaseq to generate 150 PE reads. Demultiplexed FASTQ files were mapped to the hg38 reference genome using the STAR package, and cluster analyses were performed using a cell ranger and BBrowser2 software. QC analysis was performed by removing the reads corresponding to ribosomal and mitochondrial genes, as well as cells that had less than 1X mean absolute deviation (MAD), resulting in 4705 cells that were used for further analyses. Cells were separated into clusters based on the gene expression normalization via PCA and TSNE analyses using the Seurat tool and/or Louvain clustering when using BBrowser2 software. DEG analysis identified subsets of RGCs with markers like MAP2, RBPMS, TUJ1, BRN3A, SOX4, TUBB3, SNCG, PAX6 and NRN1 in iPSC-RGCs. Differential expression analysis between separate clusters identified significant DEG transcripts associated with cell cycle, neuron regulatory networks, protein kinases, calcium signaling, growth factor hormones, and homeobox transcription factors. Further cluster refinement identified RGC diversity and subtype specification within iPSC-RGCs. DEGs can be used as biomarkers for RGC subtype classification, which will allow screening model systems that represent a spectrum of diseases with RGC pathology.

Sign in / Sign up

Export Citation Format

Share Document