scholarly journals Corneal Sulfated Glycosaminoglycans and Their Effects on Trigeminal Nerve Growth Cone Behavior In Vitro: Roles for ECM in Cornea Innervation

2012 ◽  
Vol 53 (13) ◽  
pp. 8118 ◽  
Author(s):  
Tyler Schwend ◽  
Ryan J. Deaton ◽  
Yuntao Zhang ◽  
Bruce Caterson ◽  
Gary W. Conrad
Keyword(s):  
Trigeminal Nerve ◽  
Growth Cone ◽  
Nerve Growth ◽  
Nerve Growth Cone

scholarly journals Tubulin is phosphorylated at tyrosine by pp60c-src in nerve growth cone membranes.

1990 ◽  
Vol 111 (5) ◽  
pp. 1959-1970 ◽  
Author(s):  
W T Matten ◽  
M Aubry ◽  
J West ◽  
P F Maness
Keyword(s):  
Growth Cone ◽  
Cortical Neurons ◽  
Specific Protein ◽  
Beta Tubulin ◽  
Nerve Growth ◽  
Alpha Tubulin ◽  
Nerve Growth Cone ◽  
Phosphopeptide Mapping

We show here that tubulin is the major in vivo substrate of the tyrosine-specific protein kinase pp60c-src in nerve growth cone membranes. Phosphotyrosine antibodies were used to demonstrate phosphotyrosyl residues in a subpopulation of alpha- and beta-tubulin that was highly enriched in a subcellular fraction of growth cone membranes from fetal rat brain. The presence of phosphotyrosine-modified isoforms of alpha- and beta-tubulin in vivo was confirmed by 32p labeling of rat cortical neurons in culture. Tubulin in growth cone membranes was phosphorylated at tyrosine in endogenous membrane phosphorylation reactions (0.068 mol phosphotyrosine/mol alpha-tubulin and 0.045 mol phosphotyrosine/mol beta-tubulin), and phosphorylation was specifically inhibited by antibodies directed against pp60c-src, which is localized in the growth cone membranes. pp60c-src was capable of directly phosphorylating tubulin as shown in immune complex kinase assays with purified brain tubulin. Phosphopeptide mapping revealed a limited number of sites of tyrosine phosphorylation in alpha- and beta-tubulin, with similar phosphopeptides observed in vivo and in vitro. These results reveal a novel posttranslational modification of tubulin that could regulate microtubule dynamics at the growth cone.

scholarly journals Radixin Is Involved in Lamellipodial Stability during Nerve Growth Cone Motility

1999 ◽  
Vol 10 (5) ◽  
pp. 1511-1520 ◽  
Author(s):  
Leslie Castelo ◽  
Daniel G. Jay
Keyword(s):  
Growth Cone ◽  
Critical Role ◽  
Leading Edge ◽  
Growth Cones ◽  
Forward Movement ◽  
Nerve Growth ◽  
Nerve Growth Cone

Immunocytochemistry and in vitro studies have suggested that the ERM (ezrin-radixin-moesin) protein, radixin, may have a role in nerve growth cone motility. We tested the in situ role of radixin in chick dorsal root ganglion growth cones by observing the effects of its localized and acute inactivation. Microscale chromophore-assisted laser inactivation (micro-CALI) of radixin in growth cones causes a 30% reduction of lamellipodial area within the irradiated region whereas all control treatments did not affect lamellipodia. Micro-CALI of radixin targeted to the middle of the leading edge often split growth cones to form two smaller growth cones during continued forward movement (>80%). These findings suggest a critical role for radixin in growth cone lamellipodia that is similar to ezrin function in pseudopodia of transformed fibroblasts. They are consistent with radixin linking actin filaments to each other or to the membrane during motility.

scholarly journals Plasmalemmal insertion and modification of sodium channels at the nerve growth cone

1992 ◽  
Vol 12 (8) ◽  
pp. 2948-2959 ◽  
Author(s):  
MR Wood ◽  
J DeBin ◽  
GR Strichartz ◽  
KH Pfenninger
Keyword(s):  
Sodium Channels ◽  
Growth Cone ◽  
Nerve Growth ◽  
Nerve Growth Cone

scholarly journals c-src gene product in developing rat brain is enriched in nerve growth cone membranes.

1988 ◽  
Vol 85 (14) ◽  
pp. 5001-5005 ◽  
Author(s):  
P. F. Maness ◽  
M. Aubry ◽  
C. G. Shores ◽  
L. Frame ◽  
K. H. Pfenninger
Keyword(s):  
Rat Brain ◽  
Gene Product ◽  
Growth Cone ◽  
Nerve Growth ◽  
Src Gene ◽  
Nerve Growth Cone ◽  
Developing Rat

Characterization of Phospholipase A2 Activity Enriched in the Nerve Growth Cone

2002 ◽  
Vol 67 (6) ◽  
pp. 2599-2608 ◽  
Author(s):  
Pascale Nègre-Aminou ◽  
Raphael A. Nemenoff ◽  
Malcolm R. Wood ◽  
B. A. De La Houssaye ◽  
K. H. Pfenninger
Keyword(s):  
Phospholipase A2 ◽  
Growth Cone ◽  
Nerve Growth ◽  
Nerve Growth Cone ◽  
Phospholipase A2 Activity

scholarly journals Membrane Tension, Myosin Force, and Actin Turnover Maintain Actin Treadmill in the Nerve Growth Cone

2012 ◽  
Vol 102 (7) ◽  
pp. 1503-1513 ◽  
Author(s):  
Erin M. Craig ◽  
David Van Goor ◽  
Paul Forscher ◽  
Alex Mogilner
Keyword(s):  
Growth Cone ◽  
Membrane Tension ◽  
Nerve Growth ◽  
Actin Turnover ◽  
Nerve Growth Cone
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