Corneal Epithelial Immune Dendritic Cell Alterations in Subtypes of Dry Eye Disease: A Pilot In Vivo Confocal Microscopic Study

Purpose. To evaluate the efficacy of self-retained cryopreserved amniotic membrane (CAM) in promoting corneal nerve regeneration and improving corneal sensitivity in dry eye disease (DED).Methods. In this prospective randomized clinical trial, subjects with DED were randomized to receive CAM (study group) or conventional maximum treatment (control). Changes in signs and symptoms, corneal sensitivity, topography, and in vivo confocal microscopy (IVCM) were evaluated at baseline, 1 month, and 3 months.Results. Twenty subjects (age 66.9 ± 8.9) were enrolled and 17 completed all follow-up visits. Signs and symptoms were significantly improved in the study group yet remained constant in the control. IVCM showed a significant increase in corneal nerve density in the study group (12,241 ± 5083 μm/mm2at baseline, 16,364 ± 3734 μm/mm2at 1 month, and 18,827 ± 5453 μm/mm2at 3 months,p=0.015) but was unchanged in the control. This improvement was accompanied with a significant increase in corneal sensitivity (3.25 ± 0.6 cm at baseline, 5.2 ± 0.5 cm at 1 month, and 5.6 ± 0.4 cm at 3 months,p<0.001) and corneal topography only in the study group.Conclusions. Self-retained CAM is a promising therapy for corneal nerve regeneration and accelerated recovery of the ocular surface health in patients with DED. The study is registered at clinicaltrials.gov with trial identifier:NCT02764814.

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Evaluation of Corneal Epithelial Thickness and Dry Eye Disease Tests in Thalassemic Adolescents

Clinical Ophthalmology ◽

10.2147/opth.s276335 ◽

2021 ◽

Vol Volume 15 ◽

pp. 1425-1431

Author(s):

Weam Mohamed Ebeid ◽

Mahmoud Adel Kenny ◽

Tamer AbdelFattah Badran

Keyword(s):

Dry Eye ◽

Dry Eye Disease ◽

Eye Disease ◽

Corneal Epithelial ◽

Epithelial Thickness ◽

Corneal Epithelial Thickness

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A new approach of ocular nebulization with vitamin B12 versus oxytocin for the treatment of dry eye disease: an in vivo confocal microscopy study

Drug Design Development and Therapy ◽

10.2147/dddt.s203464 ◽

2019 ◽

Vol Volume 13 ◽

pp. 2381-2391

Author(s):

Jiarui Yang ◽

Yushi Liu ◽

Yanhui Xu ◽

Xiaodan Li ◽

Jiayu Fu ◽

...

Keyword(s):

Confocal Microscopy ◽

Vitamin B12 ◽

Dry Eye ◽

Microscopy Study ◽

Dry Eye Disease ◽

Eye Disease ◽

In Vivo Confocal Microscopy ◽

New Approach

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Achyranthis radix Extract Improves Urban Particulate Matter-Induced Dry Eye Disease

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16183229 ◽

2019 ◽

Vol 16 (18) ◽

pp. 3229 ◽

Cited By ~ 2

Author(s):

Tae Gu Lee ◽

Soo-Wang Hyun ◽

Kyuhyung Jo ◽

Bongkyun Park ◽

Ik Soo Lee ◽

...

Keyword(s):

Particulate Matter ◽

Dry Eye ◽

Water Extract ◽

Dry Eye Disease ◽

Hot Water ◽

Eye Disease ◽

Corneal Epithelial ◽

Urban Particulate Matter ◽

Eye Health ◽

Induced Apoptosis

Dry eye disease (DED) is a multifactorial inflammatory disease that severely impairs patients’ quality of life. Particulate matter comprises a harmful mixture of particles less than 10 μm in size, which on contact with the eye, causes inflammation in the cornea/conjunctival epithelium, threatening eye health and triggering the onset of DED. Achyranthis radix is an ingredient of traditional medicine generally used for treating osteoporosis, trauma, and thrombosis in Asian countries. However, the effect of Achyranthis radix on eye health has not been elucidated. In this study, we evaluate the protective effect of Achyranthis radix hot water extract (ARE) in a rat model of urban particulate matter (UPM)-induced DED. UPM with or without ARE were topically administered on both eyes thrice daily for 10 days. ARE induced tear secretion and improved corneal irregularity. Additionally, ARE treatment protected the corneal epithelial cells from UPM-induced apoptosis. It also restored rMuc4 expression in the cornea and increased goblet cell density in the conjunctiva. These results are suggestive of the potential of ARE as a topical therapeutic agent for treating DED.

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Intravital Multiphoton Microscopy of the Ocular Surface: Alterations in Conventional Dendritic Cell Morphology and Kinetics in Dry Eye Disease

Frontiers in Immunology ◽

10.3389/fimmu.2020.00742 ◽

2020 ◽

Vol 11 ◽

Cited By ~ 1

Author(s):

Arsia Jamali ◽

Yashar Seyed-Razavi ◽

Cecilia Chao ◽

Gustavo Ortiz ◽

Brendan Kenyon ◽

...

Keyword(s):

Dendritic Cell ◽

Dry Eye ◽

Cell Morphology ◽

Ocular Surface ◽

Multiphoton Microscopy ◽

Dry Eye Disease ◽

Eye Disease

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New model to better diagnose dry eye disease integrating OCT corneal epithelial mapping

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2021-318826 ◽

2021 ◽

pp. bjophthalmol-2021-318826

Author(s):

Norah A Edorh ◽

Adil El Maftouhi ◽

Zoubir Djerada ◽

Carl Arndt ◽

Alexandre Denoyer

Keyword(s):

Dry Eye ◽

Roc Curves ◽

Dry Eye Disease ◽

Eye Disease ◽

Control Group ◽

Scoring Method ◽

Corneal Epithelial ◽

Epithelial Thickness ◽

Final Multivariable Model ◽

The Difference

PurposeTo optimise the objective diagnosis of dry eye disease (DED), the capabilities of wide corneal epithelial mapping using optical coherence tomography (OCT) were studied and subsequently integrated into a new scoring method.MethodsFifty-nine patients (118 eyes) with DED and 55 control subjects (110 eyes) were included. All patients underwent a complete ocular surface evaluation. Corneal epithelial thickness was collected using OCT for seven zones. DED and the control group were compared using a t-test, and univariate receiver operating characteristic (ROC) curves were calculated to define the diagnostic ability of OCT epithelial mapping. Multivariate analyses were performed using artificial intelligence (random forest) and logistic regression approaches to define the best way to integrate OCT mapping in the diagnosis of DED. Then, a final multivariable model for diagnosing DED was validated through a bootstrapping method.ResultsThe DED group had significant epithelial thinning compared with the controls, regardless of location. Superior intermediate epithelial thickness was the best marker for diagnosing DED using OCT (binormal estimated area under ROC: 0.87; best cut-off value: 50 µm thickness). The difference between the inferior and superior peripheral zones was the best marker for grading the severity of DED (analysis of variance, p=0.009). A multivariate approach identified other significant covariables which were integrated into a multivariate model to improve the sensitivity (86.4%) and specificity (91.7%) of this innovative diagnostic method.ConclusionIncluding OCT corneal epithelial mapping in a new diagnostic tool for DED could allow optimisation of the screening and staging of the disease in current practice as well as for clinical research purposes.

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