scholarly journals A Multiplex Dot Hybridization Assay For Detection and Differentiation ofAcanthamoebaand Herpes Keratitis

2016 ◽  
Vol 57 (4) ◽  
pp. 2158 ◽  
Author(s):  
Ming-Tse Kuo ◽  
Po-Chiung Fang ◽  
Hun-Ju Yu ◽  
Tsae-Ling Chao ◽  
Chun-Chih Chien ◽  
...  
Keyword(s):  
Hybridization Assay ◽  
Dot Hybridization ◽  
Herpes Keratitis

Biotin–psolaren labeled cDNA amplicons for genotyping rotavirus strains by dot hybridization assay

2007 ◽  
Vol 140 (1-2) ◽  
pp. 228-231
Author(s):  
André Luiz S. Domingues ◽  
Maria Helena Silva ◽  
Vera Gouvea
Keyword(s):  
Hybridization Assay ◽  
Dot Hybridization

scholarly journals Dot hybridization assay for distinction of rotavirus serotypes.

1989 ◽  
Vol 27 (1) ◽  
pp. 29-34 ◽  
Author(s):  
J Flores ◽  
K Y Green ◽  
D Garcia ◽  
J Sears ◽  
I Perez-Schael ◽  
...  
Keyword(s):  
Hybridization Assay ◽  
Dot Hybridization

scholarly journals A Genomic Approach to Investigating Ocular Surface Microorganisms: Monitoring Core Microbiota on Eyelid Margin with a Dot hybridization Assay

2020 ◽  
Vol 21 (21) ◽  
pp. 8299
Author(s):  
Ming-Tse Kuo ◽  
Tsai-Ling Chao ◽  
Shu-Fang Kuo ◽  
Chun-Chih Chien ◽  
Alexander Chen ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Cataract Surgery ◽  
Ocular Surface ◽  
New Technologies ◽  
Hybridization Assay ◽  
Eyelid Margin ◽  
Conventional Culture ◽  
Oxacillin Resistance ◽  
Dot Hybridization ◽  
Genomic Approach

A sound ocular surface microbiota has been recognized as a part of ocular surface health following a growing body of evidence from next-generation sequencing technique and metagenomic analysis. However, even from the perspective of contemporary precision medicine, it is difficult to directly apply these new technologies to clinical practice. Therefore, we proposed a model based on dot hybridization assay (DHA) to bridge conventional culture with a metagenomic approach in investigating and monitoring ocular surface microbiota. Endophthalmitis, mostly caused by bacterial infection, is the most severe complication of many intraocular surgeries, such as cataract surgery. Hazardous microorganisms hiding and proliferating in the ocular surface microbiota not only increase the risk of endophthalmitis but also jeopardize the effectiveness of the preoperative aseptic procedure and postoperative topical antibiotics. The DHA model enables the simultaneous assessment of bacterial bioburden, detection of target pathogens and microorganisms, and surveillance of methicillin/oxacillin resistance gene mecA in the ocular surface microbiota. This assay revealed heavier bacterial bioburden in men, compatible with a higher risk of endophthalmitis in male patients who underwent cataract surgery. No occurrence of endophthalmitis for these patients was compatible with non-hazardous microorganisms identified by specific dots for target pathogens. Moreover, the mecA dot detected oxacillin-resistant strains, of which culture failed to isolate. Therefore, the DHA model could provide an alternative genomic approach to investigate and monitor ocular surface microorganisms in clinical practice nowadays.

Rapid diagnosis of rabies infection by means of a dot hybridization assay

1988 ◽  
Vol 2 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Alain Ermine ◽  
Noël Tordo ◽  
Henri Tsiang
Keyword(s):  
Rapid Diagnosis ◽  
Hybridization Assay ◽  
Dot Hybridization

Rapid control of purity for the cytoplasm of male-sterile seed stocks by means of a dot hybridization assay

1991 ◽  
Vol 5 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Sylvain Santoni ◽  
Patricia Faivre-Rampant ◽  
Eric Moreau ◽  
André Bervillé
Keyword(s):  
Hybridization Assay ◽  
Male Sterile ◽  
Dot Hybridization ◽  
Rapid Control ◽  
Sterile Seed

scholarly journals Specificity of dot hybridization assay in the presence of rRNA for detection of rotaviruses in clinical specimens.

1987 ◽  
Vol 25 (9) ◽  
pp. 1809-1811 ◽  
Author(s):  
J Eiden ◽  
S Sato ◽  
R Yolken
Keyword(s):  
Hybridization Assay ◽  
Clinical Specimens ◽  
Dot Hybridization

scholarly journals Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe

1992 ◽  
Vol 108 (1) ◽  
pp. 175-184 ◽  
Author(s):  
J. Fernández ◽  
A. Sandino ◽  
A. Yudelevich ◽  
L. F. Avendaño ◽  
A. Venegas ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Hybridization Assay ◽  
Hybridization Method ◽  
Dot Blot ◽  
Laboratory Procedure ◽  
Dot Blot Hybridization ◽  
Gene Coding ◽  
Dot Hybridization ◽  
Stool Specimens ◽  
Higher Sensitivity

SUMMARYA synthetic oligodeoxynucletide of 40 nucleotides corresponding to nucleotides 33–72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radiactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.

Electrochemical Aptasensor Based on Proximity-Dependent Surface Hybridization Assay for Single-Step, Reusable, Sensitive Protein Detection

2007 ◽  
Vol 129 (50) ◽  
pp. 15448-15449 ◽  
Author(s):  
Yan-Li Zhang ◽  
Yong Huang ◽  
Jian-Hui Jiang ◽  
Guo-Li Shen ◽  
Ru-Qin Yu
Keyword(s):  
Protein Detection ◽  
Single Step ◽  
Hybridization Assay ◽  
Electrochemical Aptasensor
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