scholarly journals Development of a Quantitative Immunoassay for Tear Lacritin Proteoforms

2020 ◽  
Vol 9 (9) ◽  
pp. 13
Author(s):  
Brooke M. Justis ◽  
Casey E. Coburn ◽  
Ethan M. Tyler ◽  
Ryan S. Showalter ◽  
Brianna J. Dissler ◽  
...  
Keyword(s):  
Quantitative Immunoassay

Light Absorption Measurement with a CMOS Biochip for Quantitative Immunoassay Based Point-of-Care Applications

2021 ◽  
pp. 1-1
Author(s):  
Alexander Hofmann ◽  
Michael Meister ◽  
Alexander Rolapp ◽  
Peggy Reich ◽  
Friedrich Scholz ◽  
...  
Keyword(s):  
Light Absorption ◽  
Point Of Care ◽  
Absorption Measurement ◽  
Quantitative Immunoassay

iQuant™ Analyser: A rapid quantitative immunoassay reader

2017 ◽  
Author(s):  
Jayaraj Joseph ◽  
Jayaraman Kiruthi Vasan ◽  
Malay Shah ◽  
Mohansankar Sivaprakasam ◽  
Lalit Mahajan
Keyword(s):  
Quantitative Immunoassay

scholarly journals A Quantitative Immunoassay for the Lysine-Binding Function of Lipoprotein(a)

1996 ◽  
Vol 16 (5) ◽  
pp. 656-664 ◽  
Author(s):  
Jane L. Hoover-Plow ◽  
Nataya Boonmark ◽  
Pamela Skocir ◽  
Richard Lawn ◽  
Edward F. Plow
Keyword(s):  
Lipoprotein A ◽  
Binding Function ◽  
Quantitative Immunoassay

Workshop no. 3. Serodiagnosis of parasitic infections

Parasitology ◽  
1981 ◽  
Vol 82 (4) ◽  
pp. 49-55 ◽  
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Developed Countries ◽  
Parasitic Infections ◽  
Active Infection ◽  
Technical Problems ◽  
Bacterial Diseases ◽  
Quantitative Immunoassay ◽  
Long Time ◽  
Tropical Infections

Although methods for quantitative immunoassay have been available for a long time and have been used as a diagnostic tool for viral and bacterial diseases, as well as for immunological disorders, it is only recently that such methods have found wider application in parasitology. There are probably several reasons for this. One is that in developed countries parasitic diseases and in particular tropical infections were for a long time not recognized as severe health problems which deserved serious attention. Many technical problems have also been associated with parasite serology. Earlier serological methods were, for various reasons, unsuitable for use in the diagnosis of parasitic infections. For instance, the complement fixation test is, in many instances, not sensitive enough to detect active infection and the later introduced passive haemagglutination test, which has a considerably higher level of sensitivity, has the disadvantage of not detecting antibodies to carbohydrate antigens, such as some of the diagnostic antigens of adult schistosomes.

Enzyme immunochromatography--a quantitative immunoassay requiring no instrumentation.

1985 ◽  
Vol 31 (7) ◽  
pp. 1144-1150 ◽  
Author(s):  
R F Zuk ◽  
V K Ginsberg ◽  
T Houts ◽  
J Rabbie ◽  
H Merrick ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Matrix Effects ◽  
Biological Fluids ◽  
Test Strip ◽  
Paper Strip ◽  
Assay Result ◽  
Quantitative Immunoassay ◽  
Colored Zone ◽  
Enzyme Conjugate ◽  
Instrumental Methods

Abstract We describe a novel test-strip immunoassay for quantifying drugs in biological fluids. This enzyme immunochromatographic ("immunograph") method combines many features of the enzyme-channeling homogeneous immunoassay with immunochromatography and capillary migration to provide a non-separation, non-instrumental assay for theophylline in which quantification is based on the spatial distribution of enzyme label rather than on the modulation of enzyme activity. Sample antigen and hapten-enzyme conjugate are combined and moved by capillary action up a paper strip on which specific antibody has been immobilized. After color development, the assay result is evaluated by measuring the height of the colored zone on the test strip. Quantification is not a function of enzyme activity, so the method is relatively insensitive to sample matrix effects, enzyme instability, temperature, and incubation timing. Either whole blood or plasma can be used as sample. Results correlate well with those by established instrumental methods. The simple, rapid (15 min), two-incubation protocol is well suited for on-site testing in non-laboratory environments.

scholarly journals Quantitative immunoassay for complexes of prostate-specific antigen with alpha2-macroglobulin

1996 ◽  
Vol 42 (4) ◽  
pp. 545-550 ◽  
Author(s):  
F España ◽  
J Sánchez-Cuenca ◽  
A Estellés ◽  
J Gilabert ◽  
J H Griffin ◽  
...  
Keyword(s):  
Detection Limit ◽  
Prostate Specific Antigen ◽  
Protein C ◽  
Seminal Fluid ◽  
Specific Antigen ◽  
The Other ◽  
Diagnostic Significance ◽  
Healthy Men ◽  
Free Psa ◽  
Quantitative Immunoassay

Abstract We have developed two ELISAs for quantifying complexes of prostate-specific antigen (PSA) with alpha2-macroglobulin (alpha2M), using partially purified PSA:alpha2M complex as the calibrator. One ELISA was designed to evaluate )SA:alpha2M complex in fluids containing a huge excess of PSA over the amount of complex (semen-derived fluids), the other for use in fluids containing an excess of alpha2M over PSA (blood plasma). The range of the assays was 2-1000 micrograms/L for PSA complexed to alpha2M; the detection limit was 3 micrograms/: Intra- and interassay CVs were 7-13% and 11-17%, respectively, at complexed PSA concentrations of 6-500 micrograms/L. Seminal fluid from healthy men (n = 60) contained 5.2 +/- 2.6 micrograms/L PSA complexed with alpha2M. Prostatic and seminal vesicle fluids contained 6.5 +/- 2.9 ad 0.3 +/- 0.2 mg/L PSA complexed to alpha2M, respectively. When purified PSA was incubated with citrated plasma, between 45% and 65% of the added PSA was recovered as free PSA, whereas approximately 25% formed complexes with alpha2M, 10% complexed with alpha1-antichymotrypsin, and only 0.1-6% was complexed with protein C inhibitor. Of 30 patients with prostate disease, 20 showed detectable plasma PSA:alpha2M complexes; however, the potential diagnostic significance of this complex requires further investigation.

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