Residual Fluoride Concentrations and Scanning Electron Microscopic Examination of Root Surfaces of Human Teeth After Topical Application of Fluoride In Vivo

1975 ◽  
Vol 54 (4) ◽  
pp. 897-900 ◽  
Author(s):  
J. Ehrligh ◽  
N. Hochman ◽  
I. Gedalia ◽  
M. Tal
Keyword(s):  
Topical Application ◽  
Microscopic Examination ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Human Teeth ◽  
Scanning Electron Microscopic ◽  
Root Surfaces ◽  
Scanning Electron

Cell interactions of Listeria monocytogenes L forms and peritoneal exudative cells in rats

1993 ◽  
Vol 39 (11) ◽  
pp. 1014-1021 ◽  
Author(s):  
L. Mihailova ◽  
N. Markova ◽  
T. Radoucheva ◽  
D. Veljanov ◽  
S. Radoevska
Keyword(s):  
Listeria Monocytogenes ◽  
Peritoneal Cavity ◽  
Electron Microscopic Examination ◽  
Lavage Fluid ◽  
Host Cells ◽  
Electron Microscopic ◽  
Cellular Defense ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Listeria monocytogenes 4b and its forms without cell walls (L forms of a protoplastic type) were used to study in vivo interactions with host cells. Samples of peritoneal lavage fluid were obtained from rats intraperitoneally inoculated at intervals between 1 and 15 days after challenge, for scanning electron microscopic, bacteriological, biochemical, and cytometrical investigations. Scanning electron microscopic examination revealed continuous adhesion of L forms on the macrophage surface up to 15 days after inoculation. The persistence of the L forms within the peritoneal cavity was also shown bacteriologically at all sample times, while the parental bacterial forms were isolated from the peritoneal cavity up to 7 days after challenge. The total count of peritoneal exudative cells determined by automated flow peroxidase cytometry peaked on the 15th day in animals infected with parental forms, while in animals infected with L forms the peak was lower and the macrophage population was predominant. The glycolytic and acid phosphatase activity of peritoneal exudative cells was two times higher in rats infected with L forms as compared with rats infected with the L. monocytogenes parental forms on the 3rd day after challenge. An understanding of the nature of the interactions between L forms of L. monocytogenes and peritoneal exudative cells found in vivo could be used to establish the influence of L forms on host cellular defense mechanisms.Key words: Listeria monocytogenes, L forms, peritoneal exudative cells, electron microscopy.

Developmental Disturbances of the Rat Molar Induced By Two Diphosphonates

1989 ◽  
Vol 3 (2) ◽  
pp. 234-240 ◽  
Author(s):  
N. Fouda ◽  
M. Caracatsanis ◽  
L. Hammarstrom
Keyword(s):  
Microscopic Examination ◽  
Alveolar Bone ◽  
Morphological Changes ◽  
Electron Microscopic Examination ◽  
Enamel Matrix ◽  
Electron Microscopic ◽  
Developmental Disturbances ◽  
Scanning Electron Microscopic ◽  
Rat Molar ◽  
Scanning Electron

Very few reports have been published about the effects of diphosphonates on the cells and tissues of developing teeth. The present study was designed to investigate possible morphological changes in ameloblasts and odontoblasts and relate these changes to defects in the enamel surface of erupted teeth. Young rats were injected subcutaneously with single or multiple doses of HEDP or Cl2MDP (10 mg P/kg b.w.). Light microscopic examination of developing maxillary first molars showed that single injections of HEDP or Cl2MDP induced subameloblastic cysts between the secretory ameloblasts and the developing enamel. The ameloblastic lining of the cysts contained numerous calcified deposits. A few days after injection, hypoplasias were seen in the enamel in areas previously occupied by cysts. In the erupted teeth, scanning electron microscopic examination revealed enamel hypoplasias which were mainly localized on the mesial sides of the cusps. In addition to the previously mentioned disturbances, multiple injections resulted in more extensive cysts, some of which contained non-mineralized enamel matrix. Inhibition of the mineralization of dentin and alveolar bone was also noticed.

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