Fluorescence In Situ Hybridization (FISH): A Powerful Method for the Detection of Genetic Abnormalities in Metaphases and in Interphase Nuclei

1999 ◽  
Vol 26 (3) ◽  
pp. 146-150
Author(s):  
J. Dierlamm ◽  
I. Wlodarska ◽  
L. Michaux ◽  
M. Stefanova ◽  
D. Seeger ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Powerful Method ◽  
Interphase Nuclei ◽  
Genetic Abnormalities

scholarly journals Suspension (S)-FISH, a New Technique for Interphase Nuclei

2002 ◽  
Vol 50 (12) ◽  
pp. 1697-1698 ◽  
Author(s):  
Ulf Steinhaeuser ◽  
Heike Starke ◽  
Angela Nietzel ◽  
Joerg Lindenau ◽  
Peter Ullmann ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nucleus ◽  
New Technique ◽  
Interphase Nuclei ◽  
First Results ◽  
A New Technique

We describe a versatile method for performing fluorescence in situ hybridization (FISH) in suspension instead of on a slide as usually done. This so-called suspension-FISH (S-FISH) opens new possibilities for the analysis of shape and functions of the human interphase nucleus. The procedure is described and the first results using this approach are presented.

scholarly journals Procedure for whole mount fluorescence in situ hybridization of interphase nuclei on Arabidopsis thaliana

1994 ◽  
Vol 6 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Serge Bauwens ◽  
Katerina Katsanis ◽  
Marc Van Montagu ◽  
Patrick Van Oostveldt ◽  
Gilbert Engler
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nuclei ◽  
Whole Mount

Use of fluorescence in situ hybridization to study the arrangement of DNA sequences in normal and disease-related chromosomes

1995 ◽  
Vol 53 ◽  
pp. 748-749
Author(s):  
Barbara J. F. Trask ◽  
Hillary Massa ◽  
Cynthia Friedman ◽  
Richard Esposito ◽  
Ger van den Engh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Sequences ◽  
Single Copy ◽  
Two Dimensions ◽  
Genetic Maps ◽  
Epifluorescence Microscopy ◽  
Metaphase Chromosomes ◽  
Interphase Nuclei

The sites of specific DNA sequences can be fluorescently tagged by fluorescence in situ hybridization (FISH). Different sequences can be labeled with different fluorochromes so that their arrangement can be studied using epifluorescence microscopy. The distances between points on the same or different chromosomes can be determined easily in a large number of interphase nuclei or metaphase chromosomes. A variety of probe types, ranging from single-copy sequences to highly repeated sequences can be employed. Our work has focussed on the analysis of hybridization patterns in two dimensions using conventional fluorescence microscopy.We have used FISH to study various aspects of genome organization that are difficult to study using other techniques. Examples of these applications will be presented.FISH is now the method of choice for determining the chromosomal location of DNA sequences. DNA sequences can be positioned in the genome with <1:1000 accuracy (to a 3-Mbp region within a 3000-Mbp genome). Through FISH, the cytogenetic, physical and genetic maps of chromosomes can be linked.

Update on Fluorescence In Situ Hybridization in Melanoma: State of the Art

2011 ◽  
Vol 135 (7) ◽  
pp. 830-837 ◽  
Author(s):  
Pedram Gerami ◽  
Artur Zembowicz
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Personal Experience ◽  
State Of The Art ◽  
Review Of The Literature ◽  
Multicolor Fish ◽  
Melanocytic Lesions ◽  
Genetic Abnormalities

Abstract Context.—Recent advances in understanding the molecular basis of melanoma have resulted in development of fluorescence in situ hybridization (FISH) protocols designed to detect genetic abnormalities discriminating melanoma from nevi. The most extensively studied is a 4-probe multicolor FISH probe panel targeting chromosomes 6 and 11. Validation studies showed promising sensitivity and specificity for distinguishing benign nevi and malignant melanoma by FISH. Recent studies show that a melanoma FISH assay has great potential for becoming an important diagnostic adjunct in classification of melanocytic lesions and in diagnosis of melanoma. Objective.—To present a comprehensive review of the science and practical aspects of FISH in melanoma for pathologists considering the use of melanoma FISH in their practice. Data Sources.—Review of the literature and personal experience of the authors. Conclusions.—Judicious use of a 4-probe multicolor melanoma FISH procedure can enhance accuracy for diagnosis of melanoma and improve classification of melanocytic proliferations.

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