A novel use of pneumococcal urinary antigen detection kits to identify Streptococcus pneumoniae cultures misidentified by the VITEK-2 system

Streptococcus pneumoniae infections continue to be an important cause of morbidity and mortality in low-and middle-income countries. Differentiating S. pneumoniae from viridans group streptococci is essential to ensure appropriate antibiotic therapy. Conventional microbial identification tests can often misidentify the two groups. We used a common pneumococcal urinary antigen test to identify S. pneumoniae that were misidentified by the VITEK 2. The performance of the test was similar to the pneumococcal latex agglutination test.

Download Full-text

Do clinicians consider the results of the BinaxNOW Streptococcus pneumoniae urinary antigen test when adapting antibiotic regimens for pneumonia patients?

Clinical Microbiology and Infection ◽

10.1111/j.1469-0691.2010.03088.x ◽

2010 ◽

Vol 16 (9) ◽

pp. 1389-1393 ◽

Cited By ~ 16

Author(s):

M. Matta ◽

S. Kernéis ◽

N. Day ◽

M. Lescat ◽

A. Buu Hoi ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Antigen Test ◽

Urinary Antigen ◽

Urinary Antigen Test

Download Full-text

Pneumococcal Vaccines: Challenges and Prospects

Vaccines ◽

10.3390/vaccines7010025 ◽

2019 ◽

Vol 7 (1) ◽

pp. 25

Author(s):

Paul Licciardi ◽

Ioanna Papadatou

Keyword(s):

Streptococcus Pneumoniae ◽

Morbidity And Mortality ◽

Pneumococcal Vaccines ◽

Middle Income ◽

Middle Income Countries ◽

Common Causes ◽

Low And Middle Income

Infections with the bacterium Streptococcus pneumoniae are one of the most common causes of morbidity and mortality in children less than five years of age worldwide, mostly in low- and middle-income countries (LMICs) [...]

Download Full-text

Use of the NOW Streptococcus pneumoniae urinary antigen test in cerebrospinal fluid for rapid diagnosis of pneumococcal meningitis

Diagnostic Microbiology and Infectious Disease ◽

10.1016/s0732-8893(02)00548-5 ◽

2003 ◽

Vol 45 (4) ◽

pp. 237-240 ◽

Cited By ~ 53

Author(s):

Zmira Samra ◽

Haim Shmuely ◽

Elhanan Nahum ◽

Dina Paghis ◽

Josef Ben-Ari

Keyword(s):

Cerebrospinal Fluid ◽

Streptococcus Pneumoniae ◽

Pneumococcal Meningitis ◽

Rapid Diagnosis ◽

Antigen Test ◽

Urinary Antigen ◽

Urinary Antigen Test

Download Full-text

Usefulness of Streptococcus pneumoniae urinary antigen test recommended in the management of community-acquired pneumonia in cancer patients.

Journal of Clinical Oncology ◽

10.1200/jco.2014.32.15_suppl.e20679 ◽

2014 ◽

Vol 32 (15_suppl) ◽

pp. e20679-e20679

Author(s):

Paul Louis Woerther ◽

Sami Antoun ◽

Arif Alibay ◽

Sylvie Khan ◽

Bertrand Gachot ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Cancer Patients ◽

Community Acquired Pneumonia ◽

Antigen Test ◽

Urinary Antigen ◽

Urinary Antigen Test

Download Full-text

Discovery of a Streptococcus pneumoniae serotype 33F capsular polysaccharide locus that lacks wcjE and contains a wcyO pseudogene

10.1101/308767 ◽

2018 ◽

Author(s):

Sam Manna ◽

Eileen M. Dunne ◽

Belinda D. Ortika ◽

Casey L. Pell ◽

Mike Kama ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Capsular Polysaccharide ◽

Sequence Similarity ◽

Phylogenetic Analyses ◽

Latex Agglutination ◽

Reference Sequence ◽

Middle Income ◽

Capsule Locus ◽

Vaccine Impact ◽

Quellung Reaction

AbstractObjectivesAs part of large on-going vaccine impact studies in Fiji and Mongolia, we identified 25/2750 (0.9%) of nasopharyngeal swabs by microarray that were positive for Streptococcus pneumoniae contained pneumococci with a divergent 33F capsular polysaccharide locus (designated ‘33F-1’). We investigated the 33F-1 capsular polysaccharide locus to better understand the genetic variation and its potential impact on serotyping results.MethodsWhole genome sequencing was conducted on ten 33F-1 pneumococcal isolates. Initially, sequence reads were used for molecular serotyping by PneumoCaT. Phenotypic typing of 33F-1 isolates was then performed using the Quellung reaction and latex agglutination. Genome assemblies were used in phylogenetic analyses of each gene in the capsular locus to investigate genetic divergence.ResultsAll ten pneumococcal isolates with the 33F-1 cps locus typed as 33F by Quellung and latex agglutination. Unlike the reference 33F capsule locus sequence, DNA microarray and PneumoCaT analyses found that 33F-1 pneumococci lack the wcjE gene, and instead contain wcyO with a frameshift mutation. Phylogenetic analyses found the wzg, wzh, wzd, wze, wchA, wciG and glf genes in the 33F-1 cps locus had higher DNA sequence similarity to homologues from other serotypes than to the 33F reference sequence.ConclusionsWe have discovered a novel genetic variant of serotype 33F, which lacks wcjE and contains a wcyO pseudogene. This finding adds to the understanding of molecular epidemiology of pneumococcal serotype diversity, which is poorly understood in low and middle-income countries.

Download Full-text