Determination of the Bioavailability of [14C]-Hexaminolevulinate Using Accelerator Mass Spectrometry After Intravesical Administration to Human Volunteers

Traditionally, the bioavailability of vitamin B-12 (B12) from in vivo labeled foods was determined by labeling the vitamin with radiocobalt (57Co, 58Co or 60Co). This required use of penetrating radioactivity and sometimes used higher doses of B12 than the physiological limit of B12 absorption. The aim of this study was to determine the bioavailability and absorbed B12 from chicken eggs endogenously labeled with 14C-B12 using accelerator mass spectrometry (AMS). 14C-B12 was injected intramuscularly into hens to produce eggs enriched in vivo with the 14C labeled vitamin. The eggs, which provided 1.4 to 2.6 μg of B12 (~1.1 kBq) per serving, were scrambled, cooked and fed to 10 human volunteers. Baseline and post-ingestion blood, urine and stool samples were collected over a one-week period and assessed for 14C-B12 content using AMS. Bioavailability ranged from 13.2 to 57.7% (mean 30.2 ± 16.4%). Difference among subjects was explained by dose of B12, with percent bioavailability from 2.6 μg only half that from 1.4 μg. The total amount of B12 absorbed was limited to 0.5–0.8 μg (mean 0.55 ± 0.19 μg B12) and was relatively unaffected by the amount consumed. The use of 14C-B12 offers the only currently available method for quantifying B12 absorption in humans, including food cobalamin absorption. An egg is confirmed as a good source of B12, supplying approximately 20% of the average adult daily requirement (RDA for adults = 2.4 μg/day).

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Determination of IL-23 Pharmacokinetics by Highly Sensitive Accelerator Mass Spectrometry and Subsequent Modeling to Project IL-23 Suppression in Psoriasis Patients Treated with Anti-IL-23 Antibodies

The AAPS Journal ◽

10.1208/s12248-019-0352-8 ◽

2019 ◽

Vol 21 (5) ◽

Cited By ~ 2

Author(s):

Ting-Ting Zhang ◽

Junli Ma ◽

Kenneth R. Durbin ◽

Timothy Montavon ◽

Susan E. Lacy ◽

...

Keyword(s):

Mass Spectrometry ◽

Accelerator Mass Spectrometry ◽

Highly Sensitive

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Determination of Protein-Ligand Interactions Using Accelerator Mass Spectrometry: Modified Crosslinking Assay

Analytical Sciences ◽

10.2116/analsci.25.731 ◽

2009 ◽

Vol 25 (5) ◽

pp. 731-733 ◽

Cited By ~ 6

Author(s):

Sang Soo HAH

Keyword(s):

Mass Spectrometry ◽

Accelerator Mass Spectrometry ◽

Protein Ligand Interactions ◽

Ligand Interactions

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Sequential Injection Approach for Simultaneous Determination of Ultratrace Plutonium and Neptunium in Urine with Accelerator Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac4020319 ◽

2013 ◽

Vol 85 (18) ◽

pp. 8826-8833 ◽

Cited By ~ 17

Author(s):

Jixin Qiao ◽

Xiaolin Hou ◽

Per Roos ◽

Johannes Lachner ◽

Marcus Christl ◽

...

Keyword(s):

Mass Spectrometry ◽

Simultaneous Determination ◽

Accelerator Mass Spectrometry ◽

Sequential Injection

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Determination of the Radiocarbon Age of Parchment of the Vinland Map

Radiocarbon ◽

10.1017/s0033822200064651 ◽

2002 ◽

Vol 44 (1) ◽

pp. 45-52 ◽

Cited By ~ 29

Author(s):

D J Donahue ◽

J S Olin ◽

G Harbottle

Keyword(s):

Mass Spectrometry ◽

Atlantic Ocean ◽

Confidence Level ◽

Accelerator Mass Spectrometry ◽

Yale University ◽

Rare Book ◽

Radiocarbon Age ◽

The One ◽

Age Range

The Vinland Map, drawn on a 27.8 × 41.0 cm parchment bifolium, is housed in the Beinecke Rare Book and Manuscript Library at Yale University. In the northwest Atlantic Ocean, it shows “the Island of Vinland, discovered by Bjarni and Leif in company.” Skelton, Marston, and Painter (Skelton et al. 1965, 1995) firmly argued the map's authenticity, associating it with the Council of Basle (AD 1431–1449), that is, half a century before Columbus's voyage. Nevertheless, vigorous scholarly questioning of the map's authenticity has persisted (Washburn 1966; McCrone 1974; Olin and Towe 1976; Cahill et al. 1987; McCrone 1988; Towe 1990). We have determined the precise radiocarbon age of the map's parchment by accelerator mass spectrometry (AMS). The one-sigma calibrated calendrical date range is AD 1434 ± 11 years: the 95% confidence level age range is AD 1411–1468.

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