Transplacental Induction of Mouse Lung Tumors: Stage of Fetal Organogenesis in Relation to Frequency, Morphology, Size, and Neoplastic Progression of N-Nitrosoethylurea-Induced Tumors

Aims and background Lung cancer, a leading cause of cancer death, is associated with exposure to inhalation carcinogens, most commonly those found in tobacco smoke. We investigated the in vivo effect of dietary supplementation with a nutrient mixture containing lysine, proline, arginine, ascorbic acid, green tea extract, N-acetyl cysteine, selenium, copper and manganese on the development of urethane-induced lung tumors in male A/J mice. Methods After one week of isolation, seven-week-old male A/J mice (n = 25) weighing 17–19 g were randomly divided into three groups: group A (n = 5), group B (n = 10), and group C (n = 10). Mice in groups B and C were each given a single intraperitoneal injection of urethane (1 mg/g body weight) in saline, whereas group A mice received an injection of saline alone. Groups A and B were fed a regular diet, whereas group C was fed the same diet supplemented with 0.5% nutrient mixture. After 20 weeks, mice were sacrificed, lungs were excised and weighed, and tumors were counted and processed for histology. Results Urethane-challenged mice developed tumors. However, the mean number of tumors and the mean lung weights in the mice on the supplemented diet were significantly reduced, by 49% (P <0.0001) and 18% (P = 0.0025), respectively, compared to mice on the control diet. We observed neither significant differences in body weight gains nor in diet consumption among the mice. Pulmonary lesions were morphologically similar for both the groups (adenomas), but lesions were smaller in the test group. Conclusions The results suggest that nutrient mixture has inhibitory potential on the development of mouse lung tumors induced by urethane

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Ki-67 (MIB 5) immunostaining of mouse lung tumors induced by 4-nitroquinoline 1-oxide

Histochemistry and Cell Biology ◽

10.1007/s004180050321 ◽

1998 ◽

Vol 110 (6) ◽

pp. 589-593 ◽

Cited By ~ 20

Author(s):

T. Ito ◽

Hideaki Mitui ◽

Naoka Udaka ◽

Kohji Okudela ◽

Masayoshi Kanisawa ◽

...

Keyword(s):

Lung Tumors ◽

Mouse Lung ◽

Ki 67

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Activation of Protooncogenes in Mouse Lung Tumors

Experimental Lung Research ◽

10.3109/01902149109064426 ◽

1991 ◽

Vol 17 (2) ◽

pp. 389-400 ◽

Cited By ~ 15

Author(s):

Ming You ◽

Yian Wang ◽

Angela Lineen ◽

Gary D. Stoner ◽

Liang You ◽

...

Keyword(s):

Lung Tumors ◽

Mouse Lung

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Alteration of microRNA expression in vinyl carbamate-induced mouse lung tumors and modulation by the chemopreventive agent indole-3-carbinol

Carcinogenesis ◽

10.1093/carcin/bgp208 ◽

2009 ◽

Vol 31 (2) ◽

pp. 252-258 ◽

Cited By ~ 108

Author(s):

T. Melkamu ◽

X. Zhang ◽

J. Tan ◽

Y. Zeng ◽

F. Kassie

Keyword(s):

Lung Tumors ◽

Microrna Expression ◽

Mouse Lung ◽

Chemopreventive Agent ◽

Vinyl Carbamate

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Elevated proteinase activities in mouse lung tumors quantitated by synthetic fluorogenic substrates.

Journal of Histochemistry & Cytochemistry ◽

10.1177/27.11.41874 ◽

1979 ◽

Vol 27 (11) ◽

pp. 1505-1508 ◽

Cited By ~ 9

Author(s):

R Grabske ◽

A Azevedo ◽

R E Smith

Keyword(s):

Lung Tumor ◽

Lung Tumors ◽

Normal Lung ◽

Mouse Lung ◽

Lewis Lung ◽

Fluorogenic Substrates ◽

Ph Optimum ◽

Peptide Moiety ◽

Hydrolytic Activities ◽

Hydrolysis Of

Proteinase activities in malignant and normal lung tissues were measured using two synthetic substrates that consist of a fluorophor coupled to a peptide moiety. The hydrolysis of CBZ-Val-Lys-Lys-Arg-4-methoxy-2-naphthylamide and BZ-Gly-Gly-Arg-4-methoxy-2-naphthylamide were studied in homogenates of two types of mouse lung tumors, the Lewis lung tumor of the C57 black mouse and the KHT tumor of the C3H mouse. The activity of CBZ-Val-Lys-Lys-Arg-4-methoxy-2-naphthylamide hydrolysis had a pH optimum of 6.3 and a Km of 2.1 x 10(-4) M, required a thiol activator, and was inhibited by leupeptin suggesting the activity of a cathepsin B-like enzyme. The activity of BZ-Gly-Gly-Arg-4-methoxy-2-naphthylamide hydrolysis had a pH optimum of 6.7 and a Km of 3 x 10(-5) M. Lung tumor homogenates contained higher hydrolytic activities for both substrates than normal lung homogenates.

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