New Achievements in Human Cell Toxicology: The 20th Annual Workshop on In Vitro Toxicology

The 20th Annual Workshop on In Vitro Toxicology (Oxford, UK, September 22–24, 2002) was convened as part of a European meeting entitled Human Cell Culture 2002. The meeting was arranged by the Scandinavian Society for Cell Toxicology (SSCT), the European Tissue Culture Society and the British Prostate Group. Two sessions, which are summarised in this report, were devoted to in vitro toxicology: Human Cell Toxicology and The SSCT Free Paper Session. Outstanding experts in the field of toxicology outlined contemporary approaches in toxicity testing in their lectures. Short oral presentations demonstrated a variety of in vitro model systems and methodologies, which can be useful for investigating human toxicity, as well as for studies on mechanisms of toxicity.

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Immortalized hepatocytes as in vitro model systems for toxicity testing: the comparative toxicity of menadione in immortalized cells, primary cultures of hepatocytes and HTC hepatoma cells

Toxicology in Vitro ◽

10.1016/s0887-2333(96)00059-8 ◽

1996 ◽

Vol 10 (6) ◽

pp. 721-727 ◽

Cited By ~ 14

Author(s):

K. Anderson ◽

L. Yin ◽

C. Macdonald ◽

M.H. Grant

Keyword(s):

In Vitro Model ◽

Primary Cultures ◽

Toxicity Testing ◽

Hepatoma Cells ◽

Model Systems ◽

Comparative Toxicity ◽

Immortalized Cells ◽

Vitro Model ◽

Immortalized Hepatocytes

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Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs

Communications Biology ◽

10.1038/s42003-021-02977-3 ◽

2022 ◽

Vol 5 (1) ◽

Author(s):

Madalena Cipriano ◽

Katharina Schlünder ◽

Christopher Probst ◽

Kirstin Linke ◽

Martin Weiss ◽

...

Keyword(s):

Immune Cell ◽

Complex Model ◽

Model Systems ◽

Biological Drugs ◽

Mechanisms Of Toxicity ◽

Vitro Model ◽

On Chip ◽

Peripheral Immune Cells

AbstractDisorders of the eye leading to visual impairment are a major issue that affects millions of people. On the other side ocular toxicities were described for e.g. molecularly targeted therapies in oncology and may hamper their development. Current ocular model systems feature a number of limitations affecting human-relevance and availability. To find new options for pharmacological treatment and assess mechanisms of toxicity, hence, novel complex model systems that are human-relevant and readily available are urgently required. Here, we report the development of a human immunocompetent Choroid-on-Chip (CoC), a human cell-based in vitro model of the choroid layer of the eye integrating melanocytes and microvascular endothelial cells, covered by a layer of retinal pigmented epithelial cells. Immunocompetence is achieved by perfusion of peripheral immune cells. We demonstrate controlled immune cell recruitment into the stromal compartments through a vascular monolayer and in vivo-like cytokine release profiles. To investigate applicability for both efficacy testing of immunosuppressive compounds as well as safety profiling of immunoactivating antibodies, we exposed the CoCs to cyclosporine and tested CD3 bispecific antibodies.

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Human immunocompetent Choroid-on-Chip: a novel tool for studying ocular effects of biological drugs

10.1101/2021.05.12.443846 ◽

2021 ◽

Author(s):

Madalena Cipriano ◽

Katharina Schluender ◽

Christopher Probst ◽

Kirstin Linke ◽

Martin Weiss ◽

...

Keyword(s):

Immune Cell ◽

Complex Model ◽

Model Systems ◽

Biological Drugs ◽

Mechanisms Of Toxicity ◽

Vitro Model ◽

On Chip ◽

Peripheral Immune Cells

Disorders of the eye leading to visual impairment are a major issue that affects millions of people. On the other side ocular toxicities were described for e.g. molecularly targeted therapies in oncology and may hamper their development. Current ocular model systems feature a number of limitations affecting human-relevance and availability. To find new options for pharmacological treatment and assess mechanisms of toxicity, hence, novel complex model systems that are human-relevant and readily available are urgently required. Here, we report the development of a human immunocompetent Choroid-on-Chip (CoC), a human cell-based in vitro model of the choroid layer of the eye integrating melanocytes and microvascular endothelial cells, covered by a layer of retinal pigmented epithelial cells. Immunocompetence is achieved by perfusion of peripheral immune cells. We demonstrate controlled immune cell recruitment into the stromal compartments through a vascular monolayer and in vivo-like cytokine release profiles. To investigate applicability for both efficacy testing of immunosuppressive compounds as well as safety profiling of immunoactivating antibodies, we exposed the CoCs to cyclosporine and tested CD3 bispecific antibodies.

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