Therapy of Alveococcosis in Man

1977 ◽  
Vol 5 (5) ◽  
pp. 367-368 ◽  
Author(s):  
C E Lüdin ◽  
K Gyr ◽  
K Karoussos
Keyword(s):  
Partial Hepatectomy ◽  
Indirect Immunofluorescence ◽  
The Other ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Passive Haemagglutination ◽  
Indirect Immunofluorescence Test ◽  
Reliable Methods

Diagnostic, therapeutic and prognostic aspects are interpreted on the basis of two cases of Echinococcus alveolaris. Both patients had undergone partial hepatectomy and were subsequently treated chemotherapeutically. In one case dehydroemetine and mebendazole (Vermox®, Janssen), and in the other case metrifonate (Bilarcil®, Bayer A. G.) were used. The diagnosis of alveococcosis of the liver was demonstrated in one case histologically on the occasion of appendectomy, and in the other case by the indirect immunofluorescence test and passive haemagglutination. In case of suspected alveococcosis these serological tests are imperative, as they are the most reliable methods of demonstrating this disease. The latest trend in therapy aims at a combination of surgery and chemotherapy. Mebendazole seems to be promising as an anthelmintic agent for the treatment of alveococcosis. Remission of the disease was obtained in either case.

scholarly journals Indirect immunofluorescence test for serodiagnosis of Legionnaires disease: evidence for serogroup diversity of Legionnaires disease bacterial antigens and for multiple specificity of human antibodies

1979 ◽  
Vol 9 (3) ◽  
pp. 379-383
Author(s):  
H W Wilkinson ◽  
B J Fikes ◽  
D D Cruce
Keyword(s):  
Indirect Immunofluorescence ◽  
Direct Immunofluorescence ◽  
Immunofluorescence Test ◽  
Serological Tests ◽  
Fourfold Increase ◽  
Human Antibodies ◽  
Indirect Immunofluorescence Test ◽  
Human Sera ◽  
Legionnaires Disease ◽  
Group 2

Evidence obtained by others who used direct immunofluorescence staining to demonstrate serological differences among strains of Legionnaires disease bacterium prompted this study of parameters influencing the ability of the indirect immunofluorescence test to detect human antibodies to Legionnaires disease bacterium. A total of 25 Legionnaires disease bacterium strains, representing four serogroups, were used as immunofluorescence antigens to test selected human sera. The use of diethyl ether in preparing the antigens was discontinued when it was found that titers against ether-killed group 2 (Togus 1-like) antigens were impossible to determine. Instead, heat-killed suspensions of Legionnaires disease bacterium in 0.5% buffered normal chicken yolk sac were used to show the serogroup diversity of the strains and the serogroup specificity of the antibody response of some, but not all, patients with serological evidence of Legionnaires disease. These studies suggest that multiple antigens should be used in serological tests for Legionnaires disease. Furthermore, the fact that some sera contain antibodies that bind equally well to strains of all four serogroups implies that demonstration of a fourfold increase in titer of paired sera when tested with a single antigen should not be interpreted as evidence of infection with a strain of the same serogroup.

A study on antigen recognition by onchocerciasis patients with different clinical forms of disease

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 569-580 ◽  
Author(s):  
R. Lucius ◽  
D. W. Büttner ◽  
Christa Kirsten ◽  
H. J. Diesfeld
Keyword(s):  
Indirect Immunofluorescence ◽  
Antigen Recognition ◽  
The Other ◽  
Onchocerca Volvulus ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Sds Page ◽  
Antibody Titres ◽  
Low Levels ◽  
Yemen Arab Republic

SUMMARYSera from 40 onchocerciasis patients from the Yemen Arab Republic with either mild localized forms of onchocerciasis, intermediate or severe localized forms of the disease or generalized forms of infection were studies with rspect to their IgG and IgM response against Onchocerca volvulus antigens. Immunoblotting, performed with SDS–PAGE-separated proteins of female O. volvulus and quantified by densitometric scanning, revealed IgG and IgM antibodies against worm components in sera of all patients. Persons with intermediate or severe localized forms of onchocerciasis had a stronger IgG response against more proteins than individuals of the other group. However, some antigens (Mr 21, 23, 30, 33 kDa) induced comparable quantities of IgG in all groups. The IgM response of patients with mild localized forms of onchocerciasis was more intensive and directed against more antigensthan in the other groups. No antigens were detected that were recognized only by individuals with low levels of microfilaridermia. In all groups, varying concentrations of antibodies against cuticle, muscle/hypodermis layer and/or uterus of female O. volvulus were detected by the indirect immunofluorescence test using frozen worm sections as antigen. The highest mean antibody titres were found in patients with intermediate and severe localized forms of disease.

Recognition of a Non-HLA-ABC Antigen Present on B and T Lymphocytes and Monocytes only Detectable with the Indirect Immunofluorescence Test

Vox Sanguinis ◽  
1979 ◽  
Vol 36 (3) ◽  
pp. 150-158
Author(s):  
F. Décary ◽  
F.W.A. Verheugt ◽  
L. van Helden-Henningheim ◽  
E. von Riesz ◽  
R. Schreuder-van Gelder ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Antigen Present ◽  
B And T Lymphocytes

scholarly journals Behaviour of indirect immunofluorescence test and some cerebrospinal parameters in neurocysticercosis

1987 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Amauri Braga Simonetti ◽  
Jorge Teixeira
Keyword(s):  
Cerebrospinal Fluid ◽  
Indirect Immunofluorescence ◽  
False Positive ◽  
Clinical Evidence ◽  
Routine Analysis ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Immunofluorescent Test ◽  
Positive Results

Cerebrospinal fluid from 53 patients with clinical evidence of neurocysticercosis and 11 who suffered from several diseases were studied to evaluate the behaviour of indirect immunofluorescence test and some parameters of routine analysis. In neurocysticercosis there were pleocitosis in 88.7% of cases, eosinophilorrachia in 60.3%o, hyperproteinorrachia in 71.7% and hypoglucorrachia in 13.2%. The indirect immunofluorescence test was positive in 19.2% of cases but false-positive results were found when the samples showed xanthochromia or erythrocyte contamination. The authors discuss their results in comparison with those in literature and conclude that the immunofluorescent test is sensitive and useful in diagnosis of neurocysticercosis, except when the interferents previously mentioned are present.

Evaluation of the automated indirect immunofluorescence test for anti-dsDNA antibodies

2019 ◽  
Vol 498 ◽  
pp. 143-147 ◽  
Author(s):  
Shiji Wu ◽  
Feng Wang ◽  
Jin Huang ◽  
Botao Yin ◽  
Min Huang ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Dsdna Antibodies

Correlation between enzyme immunoassay and indirect immunofluorescence test for high titer of anti-ribosomal-P antibodies

2012 ◽  
Vol 413 (5-6) ◽  
pp. 650-652 ◽  
Author(s):  
Kyeong-Hee Kim ◽  
Bo-Ram Kim ◽  
Ji-Eun Kim ◽  
Kwang-Sook Woo ◽  
Jin-Yeong Han ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
High Titer ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Ribosomal P

Isolation of the Causative Organism of Canine Encephalitozoonosis

1978 ◽  
Vol 15 (4) ◽  
pp. 449-460 ◽  
Author(s):  
J. A. Shadduck ◽  
R. Bendele ◽  
G. T. Robinson
Keyword(s):  
Endothelial Cells ◽  
Indirect Immunofluorescence ◽  
Renal Tubule ◽  
Causative Organism ◽  
Encephalitozoon Cuniculi ◽  
Immunofluorescence Test ◽  
Indirect Immunofluorescence Test ◽  
Tubule Cells ◽  
Spontaneous Infection

Spontaneous infection with Encephalitozoon cuniculi resulted in a lethal disease in all but one of a litter of puppies in Texas. The disease was characterized by severe nonsuppurative nephritis, encephalitis and segmental vasculitis. Many protozoa were in renal tubule cells, endothelial cells and brain. The number of organisms decreased and the granulomatous character of the lesions became more prominent as the disease progressed. Sera from affected puppies and their parents reacted in an indirect immunofluorescence test with canine E. cuniculi propagated in vitro. The sera of owners of affected pups were negative.

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