Combined Treatment of 4′-EPI-Doxorubicin and Radiation on Hamster Lung Cells

1986 ◽  
Vol 72 (4) ◽  
pp. 339-344 ◽  
Author(s):  
Jasna Ban ◽  
Matija Bistrović ◽  
Željko Maričić ◽  
Krsto Kolarić
Keyword(s):  
Combined Treatment ◽  
Chinese Hamster ◽  
Drug Action ◽  
Simultaneous Action ◽  
Lung Cells ◽  
Cytotoxic Effects ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Cell Kill

The purpose of this study was to test the type and the degree of cytotoxic effects of epirubicin and radiation in combined treatment on Chinese hamster lung cells in vitro. Experiments were performed with proliferating tissue culture cells. Cell kill was determined by colony-forming ability. The maximum killing effects were obtained when simultaneous action of drug treatment and irradiation occurred. Their interaction was synergistic. Synergism depended on time of drug incubation (epirubicin present for 1 h). Slight antagonism was noted after prolonged drug action (24 h).

scholarly journals THE FINE STRUCTURE OF THE NUCLEOLUS IN MITOTIC DIVISIONS OF CHINESE HAMSTER CELLS IN VITRO

1965 ◽  
Vol 27 (2) ◽  
pp. 411-422 ◽  
Author(s):  
B. R. Brinkley
Keyword(s):  
Tissue Culture ◽  
Fine Structure ◽  
Osmium Tetroxide ◽  
Chinese Hamster ◽  
Late Prophase ◽  
Thin Sections ◽  
Chinese Hamster Cells ◽  
Culture Cells ◽  
Tissue Culture Cells

The nucleolus of Chinese hamster tissue culture cells (strain Dede) was studied in each stage of mitosis with the electron microscope. Mitotic cells were selectively removed from the cultures with 0.2 per cent trypsin and fixed in either osmium tetroxide or glutaraldehyde followed by osmium tetroxide. The cells were embedded in both prepolymerized methacrylate and Epon 812. Thin sections of interphase nucleoli revealed two consistent components; dense 150-A granules and fine fibrils which measured 50 A or less in diameter. During prophase, distinct zones which were observed in some interphase nucleoli (i.e. nucleolonema and pars amorpha) were lost and the nucleoli were observed to disperse into smaller masses. By late prophase or prometaphase, the nucleoli appeared as loosely wound, predominantly fibrous structures with widely dispersed granules. Such structures persisted throughout mitosis either free in the cytoplasm or associated with the chromosomes. At telophase, those nucleolar bodies associated with the chromosomes became included in the daughter nuclei, resumed their compact granular appearance, and reorganized into an interphase-type structure.

Chemical and organic characteristics of PM2.5 particles and their in-vitro cytotoxic effects on lung cells: The Middle East dust storms in Ahvaz, Iran

2019 ◽  
Vol 655 ◽  
pp. 434-445 ◽  
Author(s):  
Gholamreza Goudarzi ◽  
Mohammad Shirmardi ◽  
Abolfazl Naimabadi ◽  
Ata Ghadiri ◽  
Javad Sajedifar
Keyword(s):  
Middle East ◽  
Dust Storms ◽  
Lung Cells ◽  
Cytotoxic Effects

scholarly journals Analysis of the Dynein-Dynactin Interaction In Vitro and In Vivo

2003 ◽  
Vol 14 (12) ◽  
pp. 5089-5097 ◽  
Author(s):  
Stephen J. King ◽  
Christa L. Brown ◽  
Kerstin C. Maier ◽  
Nicholas J. Quintyne ◽  
Trina A. Schroer
Keyword(s):  
Cytoplasmic Dynein ◽  
Binding Assays ◽  
Microtubule Organization ◽  
Binding Domains ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Dynein Intermediate Chain ◽  
Transient Overexpression

Cytoplasmic dynein and dynactin are megadalton-sized multisubunit molecules that function together as a cytoskeletal motor. In the present study, we explore the mechanism of dynein-dynactin binding in vitro and then extend our findings to an in vivo context. Solution binding assays were used to define binding domains in the dynein intermediate chain (IC) and dynactin p150Glued subunit. Transient overexpression of a series of fragments of the dynein IC was used to determine the importance of this subunit for dynein function in mammalian tissue culture cells. Our results suggest that a functional dynein-dynactin interaction is required for proper microtubule organization and for the transport and localization of centrosomal components and endomembrane compartments. The dynein IC fragments have different effects on endomembrane localization, suggesting that different endomembranes may bind dynein via distinct mechanisms.

scholarly journals Study on Optimization of Cytoplasm Conditions for In Vitro Micronucleus Test Using Chinese Hamster Lung Cells

2018 ◽  
Vol 37 (3) ◽  
pp. 229-234
Author(s):  
Min Kyoung Paik ◽  
Areumnuri Kim ◽  
Hye Rim Shin ◽  
Kyongmi Chon ◽  
Kyung-Hun Park ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Chinese Hamster ◽  
Lung Cells

Human NADPH-P450 oxidoreductase modulates the level of cytochrome P450 CYP2D6 holoprotein via haem oxygenase-dependent and -independent pathways

2001 ◽  
Vol 356 (2) ◽  
pp. 613-619 ◽  
Author(s):  
Shaohong DING ◽  
Denggao YAO ◽  
Yusuf Y. DEENI ◽  
Brian BURCHELL ◽  
C. Roland WOLF ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cell Line ◽  
Combined Treatment ◽  
Chinese Hamster ◽  
Radical Scavenger ◽  
P450 Oxidoreductase ◽  
Haem Oxygenase ◽  
Mechanistic Basis ◽  
Rate Limiting

NADPH-P450 oxidoreductase (CPR) is essential for the activity of cytochrome P450 (P450). Previous studies demonstrated that CPR regulates the levels of various P450 isoforms in vitro. We investigated the mechanistic basis for this regulation. By transfection of Chinese hamster ovary DUKXB11 cells we obtained the cell line DUKX/2D6, which expressed human CYP2D6, a P450 isoform. Subsequently, DUKX/2D6 cells were transfected with human CPR cDNA to generate the cell line DUKX/2D6/CPR-3. Expression of recombinant CPR decreased the level of spectrally detectable CYP2D6 holoprotein in DUKX/2D6/CPR-3 cells by 70%, whereas the level of immunodetectable apoprotein remained unchanged. Addition of the radical scavenger DMSO increased levels of CYP2D6 holoenzyme in DUKX/2D6/CPR-3 cells but not in DUKX/2D6 cells. A similar effect was noted when cells were grown in the presence of hemin. Importantly, combined treatment with DMSO and hemin increased levels of CYP2D6 holoenzyme in DUKX/2D6/CPR-3 but not in DUKX/2D6 cells even further than either treatment alone. None of these treatments affected the level of immunodetectable CYP2D6. This demonstrates that expression of CPR increases production of damaging radicals but also that CPR may alter haem homoeostasis. In agreement with this, the activity of haem oxygenase, a rate-limiting enzyme in haem metabolism, was compared with that in DUKX/DHFR control cells (expressing dihydrofolate reductase), and was 3-fold higher in DUKX/2D6/CPR-3 but similar in DUKX/2D6 cells. Furthermore, treatment of cells with sodium arsenite increased levels of haem oxygenase concomitant with a marked decrease of spectrally detectable CYP2D6 and a rise in levels of ferritin, which sequesters free iron released from the destruction of haem. These data demonstrate that CPR regulates P450 activity by supplying electrons and also by altering P450 levels via radical-and haem oxygenase-mediated pathways.

Sign in / Sign up

Export Citation Format

Share Document