scholarly journals Immunohistochemical Characterization of Nonhuman Primate Ovarian Sex Cord–Stromal Tumors

2012 ◽  
Vol 49 (5) ◽  
pp. 834-838 ◽  
Author(s):  
A. Durkes ◽  
M. Garner ◽  
C. Juan-Sallés ◽  
J. Ramos-Vara
Keyword(s):  
Gene Product ◽  
Nonhuman Primate ◽  
Protein Gene ◽  
Neuron Specific Enolase ◽  
Protein Gene Product 9.5 ◽  
Stromal Tumors ◽  
Protein Gene Product ◽  
Granulosa Cell Tumors ◽  
Sex Cord Stromal Tumors

This study evaluates the immunoreactivity of 12 sex cord–stromal tumors of nonhuman primates (11 granulosa cell tumors and 1 luteoma). The markers selected are used in the characterization of gonadal tumors in dogs and other species, including cytokeratins AE1/AE3, GATA-4, inhibin-α, neuron-specific enolase, protein gene product 9.5, and vimentin. A normal nonhuman primate ovary was used as a control and to optimize immunolabeling. Staining was graded as follows: 0 (nonstaining), 1+ (< 10% positive cells), 2+ (10%–50% positive cells), and 3+ (> 50% positive cells). Calretinin, GATA-4, neuron-specific enolase, and vimentin were the most consistently expressed markers (12 of 12). Cytokeratins AE1/AE3 were also consistently expressed (11 of 12). Inhibin-α and protein gene product 9.5 were expressed in 8 and 10 sex cord–stromal tumors, respectively. Results indicate that immunoreactivity of nonhuman primate sex cord–stromal tumors is similar to that observed in other species and that calretinin, GATA-4, and neuron-specific enolase are the most consistently expressed markers in nonhuman primate sex cord–stromal tumors.

scholarly journals Affinity purification and characterization of protein gene product 9.5 (PGP9.5) from retina

1996 ◽  
Vol 318 (2) ◽  
pp. 711-716 ◽  
Author(s):  
Marco PICCININI ◽  
Adalberto MERIGHI ◽  
Renato BRUNO ◽  
Paolo CASCIO ◽  
Magda CURTO ◽  
...  
Keyword(s):  
Gene Product ◽  
Primary Structure ◽  
Protein Gene ◽  
Affinity Purification ◽  
Hydrolytic Activity ◽  
Protein Gene Product 9.5 ◽  
Protein Gene Product ◽  
Km Value ◽  
Ph Range

Protein gene product 9.5 (PGP9.5) is a cytosolic protein that is highly expressed in vertebrate neurons, which is now included in the ubiquitin C-terminal hydrolase subclass (UCH) on the basis of primary-structure homology and hydrolytic activity on the synthetic substrate ubiquitin ethyl ester (UbOEt). Some UCHs show affinity for immobilized ubiquitin, a property exploited to purify them. In this study we show that this property can also be applied to PGP9.5, since a protein has been purified to homogeneity from bovine retina by affinity chromatography on a ubiquitin–Sepharose column that can be identified with: (a) PGP9.5 with respect to molecular mass, primary structure and immunological reactivity; (b) the known UCHs with respect to some catalytic properties, such as hydrolytic activity on UbOEt, (which also characterizes PGP9.5), Km value and reactivity with cysteine and histidine-specific reagents. However, it differs with respect to other properties, e.g. inhibition by UbOEt and a wider pH range of activity.

scholarly journals Immunoreactivities to protein gene product 9.5, neurofilament protein and neuron specific enolase in nerves in the oviduct of the sexually immature ostrich, Struthio camelus

2006 ◽  
Vol 73 (2) ◽  
Author(s):  
M-C. Madekurozwa
Keyword(s):  
Gene Product ◽  
Protein Gene ◽  
Neuron Specific Enolase ◽  
Shell Gland ◽  
Protein Gene Product 9.5 ◽  
Nerve Fibres ◽  
Neurofilament Protein ◽  
Struthio Camelus ◽  
Neuronal Markers ◽  
Protein Gene Product

The present study investigated the distribution of nerves in the oviduct of the sexually immature ostrich. The presence of protein gene product 9.5, neurofilament protein and neuron specific enolase nerve fibres were demonstrated in the infundibulum, magnum, isthmus, shell gland and vagina. Nerve fibres containing protein gene product 9.5, neuron specific enolase and neurofilament protein were particularly numerous in the tunica muscularis and intermuscular connective tissue areas of the shell gland and vagina. The presence of a large number of nerves in these oviductal regions is probably important in the coordination of muscle contraction. An interesting finding of the study was the presence of protein gene product 9.5 and neuron specific enolase immunopositive nerve fibres in the walls of blood vessels. It is likely that these nerves are autonomicin nature and play a role in the regulation of blood flow. This study has shown the presence of an extensive neural network in the oviduct of the ostrich. In addition, the results of the investigation have shown that the neuronal markers protein gene product 9.5, neurofilament protein and neuron specific enolase can be used to demonstate nerve fibres in the ostrich.

scholarly journals The structure of the human gene encoding protein gene product 9.5 (PGP9.5), a neuron-specific ubiquitin C-terminal hydrolase

1990 ◽  
Vol 268 (2) ◽  
pp. 521-524 ◽  
Author(s):  
I N Day ◽  
L J Hinks ◽  
R J Thompson
Keyword(s):  
Gene Product ◽  
Protein Gene ◽  
Human Gene ◽  
Neuron Specific Enolase ◽  
Specific Protein ◽  
Protein Gene Product 9.5 ◽  
Gene Encoding ◽  
Protein Gene Product ◽  
Sequence Identity ◽  
Encoding Protein

Database search using a bovine thymus ubiquitin C-terminal hydrolase sequence indicated 54% sequence identity with the abundant human neuron-specific protein gene product 9.5 (PGP9.5), which was then shown to possess the same activity [Wilkinson, Lee, Deshpande, Duerksen-Hughes, Boss & Pohl (1989) Science 246, 670-673]. A yeast counterpart of the enzyme is also known. The human PGP9.5 gene, described here, spans 10 kb, contains nine exons and displays 5′ features some common to many genes and some common with neurofilament neuron-specific enolase and Thy-1-antigen gene 5′ regions.

scholarly journals Histomorphologic and Immunohistochemical Characterization of a Cardiac Purkinjeoma in a Bearded Seal (Erignathus barbatus)

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
G. Krafsur ◽  
E. J. Ehrhart ◽  
J. Ramos-Vara ◽  
G. Mason ◽  
F. Sarren ◽  
...  
Keyword(s):  
Gene Product ◽  
Protein Gene ◽  
Neuron Specific Enolase ◽  
Purkinje Fiber ◽  
Benign Tumors ◽  
Cardiac Tumors ◽  
Protein Gene Product 9.5 ◽  
Bearded Seal ◽  
Protein Gene Product ◽  
Microscopic Findings

The most common cardiac tumors of heart muscle are rhabdomyomas, solitary or multiple benign tumors of striated muscle origin. While cardiac rhabdomyomas are well described in human medical literature, limited information depicting the occurrence of cardiac rhabdomyomas in veterinary species exists. A case of multiple firm white nonencapsulated nodules in the heart of a bearded seal is described. Microscopic findings included cytoplasmic vacuolization with formation of spider cells, glycogen vacuoles, and striated myofibrils. These cells expressed immunoreactivity for neuron-specific enolase and protein gene product 9.5, a marker for neuronal tissue and Purkinje fiber cells. Immunoreactivity for protein gene product 9.5 along with other microscopic findings substantiates Purkinje fiber cell origin of the cardiac rhabdomyoma in the bearded seal and use of the termpurkinjeomato describe this lesion.

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