scholarly journals Affinity purification and characterization of protein gene product 9.5 (PGP9.5) from retina

1996 ◽  
Vol 318 (2) ◽  
pp. 711-716 ◽  
Author(s):  
Marco PICCININI ◽  
Adalberto MERIGHI ◽  
Renato BRUNO ◽  
Paolo CASCIO ◽  
Magda CURTO ◽  
...  
Keyword(s):  
Gene Product ◽  
Primary Structure ◽  
Protein Gene ◽  
Affinity Purification ◽  
Hydrolytic Activity ◽  
Protein Gene Product 9.5 ◽  
Protein Gene Product ◽  
Km Value ◽  
Ph Range

Protein gene product 9.5 (PGP9.5) is a cytosolic protein that is highly expressed in vertebrate neurons, which is now included in the ubiquitin C-terminal hydrolase subclass (UCH) on the basis of primary-structure homology and hydrolytic activity on the synthetic substrate ubiquitin ethyl ester (UbOEt). Some UCHs show affinity for immobilized ubiquitin, a property exploited to purify them. In this study we show that this property can also be applied to PGP9.5, since a protein has been purified to homogeneity from bovine retina by affinity chromatography on a ubiquitin–Sepharose column that can be identified with: (a) PGP9.5 with respect to molecular mass, primary structure and immunological reactivity; (b) the known UCHs with respect to some catalytic properties, such as hydrolytic activity on UbOEt, (which also characterizes PGP9.5), Km value and reactivity with cysteine and histidine-specific reagents. However, it differs with respect to other properties, e.g. inhibition by UbOEt and a wider pH range of activity.

scholarly journals Immunohistochemical Characterization of Nonhuman Primate Ovarian Sex Cord–Stromal Tumors

2012 ◽  
Vol 49 (5) ◽  
pp. 834-838 ◽  
Author(s):  
A. Durkes ◽  
M. Garner ◽  
C. Juan-Sallés ◽  
J. Ramos-Vara
Keyword(s):  
Gene Product ◽  
Nonhuman Primate ◽  
Protein Gene ◽  
Neuron Specific Enolase ◽  
Protein Gene Product 9.5 ◽  
Stromal Tumors ◽  
Protein Gene Product ◽  
Granulosa Cell Tumors ◽  
Sex Cord Stromal Tumors

This study evaluates the immunoreactivity of 12 sex cord–stromal tumors of nonhuman primates (11 granulosa cell tumors and 1 luteoma). The markers selected are used in the characterization of gonadal tumors in dogs and other species, including cytokeratins AE1/AE3, GATA-4, inhibin-α, neuron-specific enolase, protein gene product 9.5, and vimentin. A normal nonhuman primate ovary was used as a control and to optimize immunolabeling. Staining was graded as follows: 0 (nonstaining), 1+ (< 10% positive cells), 2+ (10%–50% positive cells), and 3+ (> 50% positive cells). Calretinin, GATA-4, neuron-specific enolase, and vimentin were the most consistently expressed markers (12 of 12). Cytokeratins AE1/AE3 were also consistently expressed (11 of 12). Inhibin-α and protein gene product 9.5 were expressed in 8 and 10 sex cord–stromal tumors, respectively. Results indicate that immunoreactivity of nonhuman primate sex cord–stromal tumors is similar to that observed in other species and that calretinin, GATA-4, and neuron-specific enolase are the most consistently expressed markers in nonhuman primate sex cord–stromal tumors.

Ultrastructural localization and regulation of protein gene product 9.5

Neuroreport ◽  
1997 ◽  
Vol 8 (14) ◽  
pp. 2999-3004 ◽  
Author(s):  
Whei-Min Lin ◽  
Sung-Tsang Hsieh ◽  
Ia-Tang Huang ◽  
John W. Griffin ◽  
Wen-Pin Chen
Keyword(s):  
Gene Product ◽  
Protein Gene ◽  
Ultrastructural Localization ◽  
Protein Gene Product 9.5 ◽  
Protein Gene Product

Expression of protein gene product 9.5 in the anterior lens epithelial cells of atopic cataracts

2002 ◽  
Vol 28 (11) ◽  
pp. 2035-2039 ◽  
Author(s):  
Junko Nakajima ◽  
Atsushi Mekada ◽  
Jiro Nakamura ◽  
Yasuhiro Nishida ◽  
Yoshimitsu Tokunaga
Keyword(s):  
Epithelial Cells ◽  
Gene Product ◽  
Protein Gene ◽  
Lens Epithelial Cells ◽  
Protein Gene Product 9.5 ◽  
Protein Gene Product
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