Determination of cytotoxic and genotoxic effects of naphthalene, 1-naphthol and 2-naphthol on human lymphocyte culture

2012 ◽  
Vol 30 (1) ◽  
pp. 82-89 ◽  
Author(s):  
Mete Kapuci ◽  
Zeynep Ulker ◽  
Sezin Gurkan ◽  
Lokman Alpsoy
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Genotoxic Effects

scholarly journals Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

2016 ◽  
Vol 67 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Rafael Valencia-Quintana ◽  
Sandra Gómez-Arroyo ◽  
Juana Sánchez-Alarcón ◽  
Mirta Milić ◽  
José Luis Gómez Olivares ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Human Lymphocyte ◽  
Cell Cycle Progression ◽  
Lymphocyte Culture ◽  
Root Tip ◽  
Genotoxic Effects ◽  
Animal Metabolism ◽  
Index Ratio

Abstract The aim of the study was to evaluate genotoxic effects of Pirimor-50®, a pirimicarb-based formulation (50 % active ingredient), in human lymphocyte cultures and Vicia faba root meristems. Furthermore, the objective was to examine a combined influence of insecticide treatment with mammalian microsomal S9 and vegetal S10 metabolic fractions or S10 mix metabolic transformation extracts (after Vicia faba primary roots treatment with Pirimor-50®). We used sister chromatid exchange assay-SCE and measured cell cycle progression and proliferation (proportion of M1-M3 metaphases and replication index ratio-RI). Two processes were used for plant promutagen activation: in vivo activation-Pirimor-50® was applied for 4 h to the plant and then S10 mix was added to lymphocytes; and, in vitro activation-lymphocytes were treated with Pirimor-50® and S10 or S9 for 2 h. Direct treatment induced significantly higher SCE frequencies in meristems at 0.01 mg mL-1. In lymphocytes, significantly higher SCE was at 1 mg mL-1 with decrease in RI and M1-M3 metaphase proportions at 0.5 mg mL-1 and cell division stop at 2.5 mg mL1. S10 mix lymphocyte treatment showed significantly elevated SCE values at 2-2.5 mg mL-1, with cell death at 3 mg mL-1. Lymphocyte treatment with Pirimor-50® together with S9 or S10 showed slightly elevated SCE frequency but had a significant influence on RI decrease, with lowest values in S9 treatment. Since no data are available on the genotoxicity of Pirimor-50®, this study is one of the first to evaluate and compare its direct effect in two bioassays, animal and vegetal, and also the effect of plant and animal metabolism on its genotoxic potential.

scholarly journals Analysis of Delphinidin and Luteolin Genotoxicity in Human Lymphocyte Culture

2015 ◽  
Vol 5 (2) ◽  
pp. 41-45 ◽  
Author(s):  
Jasmin Ezić ◽  
Amina Kugić ◽  
Maida Hadžić ◽  
Anja Haverić ◽  
Kasim Bajrović ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Human Lymphocyte ◽  
Chromosome Aberrations ◽  
Topoisomerase Ii ◽  
Human Lymphocytes ◽  
Lymphocyte Culture ◽  
Genotoxic Effects ◽  
Genotoxic Potential

Introduction: Bioflavonoids delphinidin (2-(3,4,5-Trihydroxyphenyl)chromenylium-3,5,7-triol) and luteolin (2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-4-chromenone) have been recognized as promising antioxidants and anticancer substances. Due to their extensive use, the goal of the research was to determine whether they have any genotoxic potential in vitro.Methods: Analysis of genotoxic potential was performed applying chromosome aberrations test in human lymphocyte culture, as this kind of research was not conducted abundantly for these two bioflavonoids. Delphinidin and luteolin were dissolved in DMSO and added to cultures in final concentrations of 25, 50 and 100 μM.Results: In human lymphocytes cultures Delphinidin induced PCDs in all treatments, potentially affecting the cell cycle and topoisomerase II activity. In concentration of 50 μM luteolin showed strong genotoxic effects and caused significant reduction of cell proliferation.Conclusion: Luteolin exhibited certain genotoxic and cytostatic potential. Delphinidin was not considered genotoxic, however its impact on mitosis, especially topoisomerase II activity, was revealed.

Anti-oxidative and anti-genotoxic effects of carnosine on human lymphocyte culture

2011 ◽  
Vol 30 (12) ◽  
pp. 1979-1985 ◽  
Author(s):  
Lokman Alpsoy ◽  
Gamze Akcayoglu ◽  
Hilal Sahin
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Human Lymphocyte ◽  
Test System ◽  
Lymphocyte Culture ◽  
Total Glutathione ◽  
Genotoxic Effects ◽  
Significant Attenuation ◽  
Oxidative Effects ◽  
Sce Test

The aim of this study was to investigate the effects of carnosine, a biological antioxidant, on the oxidative stress and genotoxicity by a single dose of carbon tetrachloride (CCl4; 5 mM) in the human lymphocyte culture. We studied the anti-genotoxic effects of carnosine by using sister chromatid exchange (SCE) test system. Also, the anti-oxidative effects of carnosine were evaluated by using superoxide dismutase (SOD), glutathione peroxidase (GPx), total glutathione (GSH) and malondialdehyde (MDA) assay. The SCE frequency was increased when treated with CCl4. Carnosine at 10 and 20 mM reduced SCE frequency in the human lymphocyte ( p < 0.001). In addition, CCl4 treatment significantly depleted the level of GSH, reduced the activity of SOD and GPx and elevated the level of MDA ( p < 0.001). Carnosine treatment led to significant attenuation of CCl4-induced oxidative stress by normalization of the activities of SOD and GPx and the level of GSH and MDA ( p < 0.05 or 0.001). These results suggest that carnosine could provide anti-oxidative and anti-genotoxic protection for the oxidative and genotoxic agents that cause many diseases including cancer and neurodegenerative disease.

scholarly journals IN VITRO GENOTOXICITY OF THREE PLANTS FROM ASTERACEAE FAMILY IN HUMAN LYMPHOCYTES CULTURES

2022 ◽  
Author(s):  
M. Kinan Aljaja ◽  
Adawia Kitaz ◽  
Raghda Lahdo
Keyword(s):  
Medicinal Plants ◽  
Peer Review ◽  
Mitotic Index ◽  
Human Lymphocyte ◽  
Human Lymphocytes ◽  
Genotoxic Effects ◽  
Medicinal Uses ◽  
Lymphocyte Cultures

Background: Onopordum carduiforme, Centaurea verutum, and Achillea santolina are medicinal plants grown in Syria and commonly used in traditional medicine. Such as antibacterial, antioxidant and anticancer properties. However, the genotoxic effects of these plants have not been studied. Aim and objective: the aim of this study was to evaluate the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures model by evaluating the cell proliferation, determination of mitotic index (MI), and their effects on chromosomes. Methods: the hydroethanolic extracts of the aerial parts of the three plants were extracted using an Ultrasonic bath. Then the genotoxic effects of hydroethanolic extracts of these plants on human lymphocyte cultures was conducting by determination of mitotic index (MI). Results: the results showed that all three plants decreased non-significantly the mean of mitotic index in comparison with negative control (normal MI) (p>0.05) at concentrations (1, 3, 5 mg/ml) and the mitotic index values ranged was between (2.25±0.07 and 3.3±0.28). However, C. verutum showed the lowest mitotic index (3±0.14 at 1 mg/ml) and (2.25±0.07 at 5 mg/ml), and did not induce chromatid or chromosome breaks or gaps. Conclusion: these preliminary results on cytotoxicity and mutagenicity of these plants provide valuable information about the safety of using them in alternative medicine.                    Peer Review History: Received: 11 November 2021; Revised: 13 December; Accepted: 28 December, Available online: 15 January 2022 Academic Editor:  Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, [email protected] Dr. Dennis Amaechi, MrsFoluBabade Mini Estate , Flat 5 by Old Soldiers Quarter, Sabongari/Bwari, Abuja- Federal Capital Territory, Nigeria. [email protected]  Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, [email protected] Similar Articles: A STUDY ON DIFFERENT PLANTS OF APOCYNACEAE FAMILY AND THEIR MEDICINAL USES STUDY LITERATION OF CHEMICAL CONTENTS OF SOME PLANTS THAT POTENTIALLY AS THE SOLAR SOWS EXPLORING THE ANTIPARASITIC ACTIVITY OF MEDICINAL PLANTS

scholarly journals Satureja subspicata and S. horvatii Extracts Induce Overexpression of the BCl-2 Family of Anti-apoptotic Genes and Reduce Micronuclei Frequency in Mice

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Jasmina Čakar ◽  
Naida Kadrić Lojo ◽  
Anja Haverić ◽  
Maida Hadžić ◽  
Lejla Lasić ◽  
...  
Keyword(s):  
Human Lymphocyte ◽  
Micronucleus Assay ◽  
Balkan Peninsula ◽  
Lymphocyte Culture ◽  
Cytotoxic Activities ◽  
Cytotoxic Effects ◽  
Apoptotic Genes ◽  
Apoptotic Activity

Satureja subspicata and S. horvatii are endemic species of the Balkan Peninsula and often used in traditional medicine in Bosnia and Herzegovina to treat different health conditions. We aimed to analyze the unevaluated apoptotic, genotoxic and cytotoxic effects of two Satureja species, as well as their content of phenolics that are mainly responsible for the plant's biological activity. Apoptotic and geno/cytotoxic activities of S. subspicata and S. horvatii were investigated in vitro in human lymphocyte culture and in vivo in mice. The content of the main phenolics in plant extracts was determined by ultra-high pressure liquid chromatography-MS-MS (UHPLC–MS/MS). Genotoxic and cytotoxic activities of Satureja extracts were evaluated in vitro by applying a cytokinesis-block micronucleus cytome assay in human lymphocyte culture and in vivo applying a mice reticulocytes micronucleus assay. SALSA RT-MLPA R011-C1 apoptosis assay was used for measuring the relative expression of 44 genes associated with the regulation of the apoptotic pathways in human lymphocyte cultures treated with different concentrations of two Satureja extracts. The first analysis of phenolic compounds in S. horvatii and S. subspicata determined by an UHPLC-MS/MS method revealed high levels of rosmarinic and caffeic acids. Minor genotoxic potential was determined in relation to the tested concentrations while no cytostatic and cytotoxic effects were revealed in vitro. However, when applied in concentrations of 200 mg/kg per os, aqueous extracts of two Satureja species significantly decreased frequency of reticulocytes micronuclei in treated mice against controls. Extracts of S. subspicata and S. horvatii in concentrations of 0.2 mg/mL, regardless of solvent used, downregulated pro-apoptotic and upregulated anti-apoptotic genes, showing anti-apoptotic activity. Our results indicate that the registered anti-genotoxic and anti-apoptotic activity is most likely related to the high level of phenolic acids (particularly rosmarinic and caffeic) in the tested extracts.

Effect of CO2 on short-term human lymphocyte culture in vitro

In Vitro ◽  
1977 ◽  
Vol 13 (11) ◽  
pp. 806-807 ◽  
Author(s):  
Ram S. Verma ◽  
Carole Rubenstein ◽  
Harvey Dosik
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Short Term ◽  
Culture In Vitro

scholarly journals Role of anti-human lymphocyte culture cytotoxic antibodies in recurrent spontaneous pregnancy loss women

2011 ◽  
Vol 4 (1) ◽  
pp. 17 ◽  
Author(s):  
Shankarkumar Umapathy ◽  
Aruna Shankarkumar ◽  
Vanita Ramrakhiyani ◽  
Kanjaksha Ghosh
Keyword(s):  
Human Lymphocyte ◽  
Pregnancy Loss ◽  
Lymphocyte Culture ◽  
Cytotoxic Antibodies ◽  
Spontaneous Pregnancy

Determination of the genotoxic effects of Convolvulus arvensis extracts on corn (Zea mays L.) seeds

2012 ◽  
Vol 29 (5) ◽  
pp. 449-459 ◽  
Author(s):  
Serap Sunar ◽  
Nalan Yildirim ◽  
Ozkan Aksakal ◽  
Guleray Agar
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Genotoxic Effects ◽  
Convolvulus Arvensis

Investigation of decomposition of alkylating mutagens of the ethylenimine series in human lymphocyte culture

1976 ◽  
Vol 81 (5) ◽  
pp. 689-691 ◽  
Author(s):  
O. P. Kirichenko ◽  
A. N. Chebotarev ◽  
K. N. Yakovenko
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Alkylating Mutagens

Determination of genotoxic effects of stanozolol in rats

2015 ◽  
Vol 238 (2) ◽  
pp. S238
Author(s):  
B. Alpertunga ◽  
M. Kara ◽  
E. Özçağlı ◽  
P. Fragkiadaki ◽  
A. Tsatsakis
Keyword(s):  
Genotoxic Effects
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