scholarly journals Role of anti-human lymphocyte culture cytotoxic antibodies in recurrent spontaneous pregnancy loss women

2011 ◽  
Vol 4 (1) ◽  
pp. 17 ◽  
Author(s):  
Shankarkumar Umapathy ◽  
Aruna Shankarkumar ◽  
Vanita Ramrakhiyani ◽  
Kanjaksha Ghosh
Keyword(s):  
Human Lymphocyte ◽  
Pregnancy Loss ◽  
Lymphocyte Culture ◽  
Cytotoxic Antibodies ◽  
Spontaneous Pregnancy

The Role of Panchakarma in recurrent pregnancy loss- A case study

2018 ◽  
Vol 6 (5th) ◽  
Author(s):  
Amol Kanodje ◽  
Vijay Kumar Nawle
Keyword(s):  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Bed Rest ◽  
First Trimester ◽  
Second Trimester ◽  
Foetal Distress ◽  
Spontaneous Pregnancy ◽  
Female Baby

Recurrent pregnancy loss is defined as three or more consecutive spontaneous losses of pregnancy. In Ayurvedic samhita “GarbhastraviVandhya” is a term for recurrent habitual abortion in the first trimester and “Garbhapata” in second trimester up to 20 weeks from the last menstrual period. Spontaneous pregnancy loss is a surprisingly common occurrence and affects 1% of couples trying to conceive. The best available data suggest that the risk of miscarriage in subsequent pregnancies is 30% after 2 losses, compared with 33% after 3 losses.1 In this case study, interpretation of cause is done on basics of Ayurvedic diagnostic parameters and management is done according to that.  Deepan, pachan, panchakarma specially Uttar vasti were planned before conception and after conception complete bed rest along with proper antenatal medicines and advices were given. There was uneventful antenatal period except foetal distress at 39.5 weeks of gestation and the patient was delivered by emergency caesarean section. A female baby with 3.1 kg birth weight was born.

Production of interferon by red deer (Cervus elaphus) conceptuses and the effects of roIFN-tau on the timing of luteolysis and the success of asynchronous embryo transfer

Reproduction ◽  
2000 ◽  
pp. 387-395 ◽  
Author(s):  
KJ Demmers ◽  
HN Jabbour ◽  
DW Deakin ◽  
AP Flint
Keyword(s):  
Embryo Transfer ◽  
Early Pregnancy ◽  
Cervus Elaphus ◽  
Pregnancy Loss ◽  
Red Deer ◽  
Interferon Tau ◽  
Recombinant Interferon ◽  
Uterine Flushing ◽  
Calving Rate

The role of interferon in early pregnancy in red deer was investigated by (a) measuring production of interferon by the conceptus, (b) testing the anti-luteolytic effect of recombinant interferon-tau in non-pregnant hinds, and (c) treatment of hinds with interferon after asynchronous embryo transfer. Blastocysts were collected from 34 hinds by uterine flushing 14 (n = 2), 16 (n = 2), 18 (n = 8), 20 (n = 13) or 22 (n = 9) days after synchronization of oestrus with progesterone withdrawal. Interferon anti-viral activity was detectable in uterine flushings from day 16 to day 22, and increased with duration of gestation (P < 0.01) and developmental stage (P < 0.01). When interferon-tau was administered daily between day 14 and day 20 to non-pregnant hinds to mimic natural blastocyst production, luteolysis was delayed by a dose of 0.2 mg day(-1) (27.3 +/- 1.3 days after synchronization, n = 4 versus 21 +/- 0 days in control hinds, n = 3; P < 0.05). Interferon-tau was administered to hinds after asynchronous embryo transfer to determine whether it protects the conceptus against early pregnancy loss. Embryos (n = 24) collected on day 6 from naturally mated, superovulated donors (n = 15) were transferred into synchronized recipients on day 10 or day 11. Interferon-tau treatment (0.2 mg daily from day 14 to 20) increased calving rate from 0 to 64% in all recipients (0/11 versus 7/11, P < 0.005), and from 0 to 67% in day 10 recipients (0/8 versus 6/9, P < 0.01). The increased success rate of asynchronous embryo transfer after interferon-tau treatment in cervids may be of benefit where mismatched embryo-maternal signalling leads to failure in the establishment of pregnancy.

scholarly journals Satureja subspicata and S. horvatii Extracts Induce Overexpression of the BCl-2 Family of Anti-apoptotic Genes and Reduce Micronuclei Frequency in Mice

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Jasmina Čakar ◽  
Naida Kadrić Lojo ◽  
Anja Haverić ◽  
Maida Hadžić ◽  
Lejla Lasić ◽  
...  
Keyword(s):  
Human Lymphocyte ◽  
Micronucleus Assay ◽  
Balkan Peninsula ◽  
Lymphocyte Culture ◽  
Cytotoxic Activities ◽  
Cytotoxic Effects ◽  
Apoptotic Genes ◽  
Apoptotic Activity

Satureja subspicata and S. horvatii are endemic species of the Balkan Peninsula and often used in traditional medicine in Bosnia and Herzegovina to treat different health conditions. We aimed to analyze the unevaluated apoptotic, genotoxic and cytotoxic effects of two Satureja species, as well as their content of phenolics that are mainly responsible for the plant's biological activity. Apoptotic and geno/cytotoxic activities of S. subspicata and S. horvatii were investigated in vitro in human lymphocyte culture and in vivo in mice. The content of the main phenolics in plant extracts was determined by ultra-high pressure liquid chromatography-MS-MS (UHPLC–MS/MS). Genotoxic and cytotoxic activities of Satureja extracts were evaluated in vitro by applying a cytokinesis-block micronucleus cytome assay in human lymphocyte culture and in vivo applying a mice reticulocytes micronucleus assay. SALSA RT-MLPA R011-C1 apoptosis assay was used for measuring the relative expression of 44 genes associated with the regulation of the apoptotic pathways in human lymphocyte cultures treated with different concentrations of two Satureja extracts. The first analysis of phenolic compounds in S. horvatii and S. subspicata determined by an UHPLC-MS/MS method revealed high levels of rosmarinic and caffeic acids. Minor genotoxic potential was determined in relation to the tested concentrations while no cytostatic and cytotoxic effects were revealed in vitro. However, when applied in concentrations of 200 mg/kg per os, aqueous extracts of two Satureja species significantly decreased frequency of reticulocytes micronuclei in treated mice against controls. Extracts of S. subspicata and S. horvatii in concentrations of 0.2 mg/mL, regardless of solvent used, downregulated pro-apoptotic and upregulated anti-apoptotic genes, showing anti-apoptotic activity. Our results indicate that the registered anti-genotoxic and anti-apoptotic activity is most likely related to the high level of phenolic acids (particularly rosmarinic and caffeic) in the tested extracts.

scholarly journals Distribution of diandric and digynic triploidy depending on gestational age

2021 ◽  
Author(s):  
Diana Massalska ◽  
Katarzyna Ozdarska ◽  
Tomasz Roszkowski ◽  
Julia Bijok ◽  
Anna Kucińska-Chahwan ◽  
...  
Keyword(s):  
Gestational Age ◽  
Pregnancy Loss ◽  
First Trimester ◽  
Parental Origin ◽  
Second Trimester ◽  
Chain Reaction ◽  
Spontaneous Pregnancy ◽  
First Trimester Of Pregnancy ◽  
Polymerase Chain ◽  
Fluorescent Polymerase Chain Reaction

Abstract Purpose To establish the distribution of diandric and digynic triploidy depending on gestational age. Methods 107 triploid samples tested prospectively in a single genetic department during a four-year period were analyzed for parental origin of triploidy by Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR) (n=95) with the use of matching parental samples or by MS-MLPA (n=12), when parental samples were unavailable. Tested pregnancies were divided into three subgroups with regard to the gestational age at spontaneous pregnancy loss: <11 gestational weeks, 11–14 gestational weeks, and >14 gestational weeks. Results Diandric triploidy constituted overall 44.9% (46.5% in samples miscarried <11 gestational weeks, 64.3% in samples miscarried between 11 and 14 gestational weeks, and 27.8% in pregnancies which survived >14 gestational weeks). Conclusions The distribution of diandric and digynic triploidy depends on gestational age. The majority of diandric triploid pregnancies is lost in the first trimester of pregnancy. In the second trimester, diandric cases are at least twice less frequent than digynic ones.

scholarly journals The role of low molecular weight heparin on recurrent pregnancy loss: A systematic review and meta-analysis

2021 ◽  
Vol 60 (1) ◽  
pp. 1-8
Author(s):  
Fangfei Jiang ◽  
Xiuying Hu ◽  
Kang Jiang ◽  
Hongxia Pi ◽  
Qiyao He ◽  
...  
Keyword(s):  
Systematic Review ◽  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Pregnancy Loss ◽  
Recurrent Pregnancy Loss ◽  
Meta Analysis ◽  
Low Molecular Weight

scholarly journals Role of amino groups in formation of human lymphocyte-xenogeneic erythrocyte rosettes; a proposed mechanism for antigen recognition.

1975 ◽  
Vol 72 (2) ◽  
pp. 697-700 ◽  
Author(s):  
P. Lalezari ◽  
S. L. Nehlsen ◽  
J. Novodoff ◽  
I. Lalezari
Keyword(s):  
Human Lymphocyte ◽  
Antigen Recognition ◽  
Amino Groups

Effect of CO2 on short-term human lymphocyte culture in vitro

In Vitro ◽  
1977 ◽  
Vol 13 (11) ◽  
pp. 806-807 ◽  
Author(s):  
Ram S. Verma ◽  
Carole Rubenstein ◽  
Harvey Dosik
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Short Term ◽  
Culture In Vitro

Pregnancy Loss and Endometriosis: Pathogenic Role of Anti-Laminin-1 Autoantibodies

2005 ◽  
Vol 1051 (1) ◽  
pp. 174-184 ◽  
Author(s):  
JUNKO INAGAKI ◽  
AKANE KONDO ◽  
LUIS R. LOPEZ ◽  
YEHUDA SHOENFELD ◽  
EIJI MATSUURA
Keyword(s):  
Pregnancy Loss ◽  
Pathogenic Role ◽  
Laminin 1
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