Effect of CO2 on short-term human lymphocyte culture in vitro

In Vitro ◽  
1977 ◽  
Vol 13 (11) ◽  
pp. 806-807 ◽  
Author(s):  
Ram S. Verma ◽  
Carole Rubenstein ◽  
Harvey Dosik
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Short Term ◽  
Culture In Vitro

Pesticide Induced Cytogenetic Risk Assessment in Human Lymphocyte Culture In Vitro

2005 ◽  
Vol 75 (1) ◽  
pp. 7-14 ◽  
Author(s):  
K. Jamil ◽  
A. P. Shaik ◽  
J. Lakshimi A.
Keyword(s):  
Risk Assessment ◽  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Culture In Vitro

scholarly journals Satureja subspicata and S. horvatii Extracts Induce Overexpression of the BCl-2 Family of Anti-apoptotic Genes and Reduce Micronuclei Frequency in Mice

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Jasmina Čakar ◽  
Naida Kadrić Lojo ◽  
Anja Haverić ◽  
Maida Hadžić ◽  
Lejla Lasić ◽  
...  
Keyword(s):  
Human Lymphocyte ◽  
Micronucleus Assay ◽  
Balkan Peninsula ◽  
Lymphocyte Culture ◽  
Cytotoxic Activities ◽  
Cytotoxic Effects ◽  
Apoptotic Genes ◽  
Apoptotic Activity

Satureja subspicata and S. horvatii are endemic species of the Balkan Peninsula and often used in traditional medicine in Bosnia and Herzegovina to treat different health conditions. We aimed to analyze the unevaluated apoptotic, genotoxic and cytotoxic effects of two Satureja species, as well as their content of phenolics that are mainly responsible for the plant's biological activity. Apoptotic and geno/cytotoxic activities of S. subspicata and S. horvatii were investigated in vitro in human lymphocyte culture and in vivo in mice. The content of the main phenolics in plant extracts was determined by ultra-high pressure liquid chromatography-MS-MS (UHPLC–MS/MS). Genotoxic and cytotoxic activities of Satureja extracts were evaluated in vitro by applying a cytokinesis-block micronucleus cytome assay in human lymphocyte culture and in vivo applying a mice reticulocytes micronucleus assay. SALSA RT-MLPA R011-C1 apoptosis assay was used for measuring the relative expression of 44 genes associated with the regulation of the apoptotic pathways in human lymphocyte cultures treated with different concentrations of two Satureja extracts. The first analysis of phenolic compounds in S. horvatii and S. subspicata determined by an UHPLC-MS/MS method revealed high levels of rosmarinic and caffeic acids. Minor genotoxic potential was determined in relation to the tested concentrations while no cytostatic and cytotoxic effects were revealed in vitro. However, when applied in concentrations of 200 mg/kg per os, aqueous extracts of two Satureja species significantly decreased frequency of reticulocytes micronuclei in treated mice against controls. Extracts of S. subspicata and S. horvatii in concentrations of 0.2 mg/mL, regardless of solvent used, downregulated pro-apoptotic and upregulated anti-apoptotic genes, showing anti-apoptotic activity. Our results indicate that the registered anti-genotoxic and anti-apoptotic activity is most likely related to the high level of phenolic acids (particularly rosmarinic and caffeic) in the tested extracts.

scholarly journals THE HISTOGENETIC AND BIOSYNTHETIC CAPABILITIES OF URODELE CHONDROCYTES AFTER SHORT-TERM AND PROLONGED CULTURE IN VITRO*

1972 ◽  
Vol 14 (4) ◽  
pp. 325-335 ◽  
Author(s):  
J. J. CHIAKULAS ◽  
T. TSAI ◽  
L. E. SCHEVING
Keyword(s):  
Short Term ◽  
Culture In Vitro ◽  
Prolonged Culture

Damage by Dichlorvos of Human Lymphocyte DNA

1980 ◽  
Vol 66 (4) ◽  
pp. 425-430 ◽  
Author(s):  
Paolo Perocco ◽  
Angela Fini
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocyte ◽  
Tritiated Thymidine ◽  
Human Lymphocytes ◽  
Ultraviolet Rays ◽  
Thymidine Uptake ◽  
Short Term ◽  
In Vitro System ◽  
Tritiated Thymidine Uptake

The action of dichlorvos (2.2-dichlorovinyldimethyl phosphate) was studied with a short-term in vitro system which utilizes human lymphocytes. The parameters studied were the action exerted by the pesticide on scheduled (semiconservative) and unscheduled (reparative) DNA synthesis measured as tritiated thymidine uptake. The results obtained show that dichlorvos affects semiconservative DNA synthesis, damages human lymphocyte DNA inducing low reparative synthesis, and interferes with DNA repair processes after damage exerted by ultraviolet rays.

scholarly journals Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts

2016 ◽  
Vol 67 (4) ◽  
pp. 266-276 ◽  
Author(s):  
Rafael Valencia-Quintana ◽  
Sandra Gómez-Arroyo ◽  
Juana Sánchez-Alarcón ◽  
Mirta Milić ◽  
José Luis Gómez Olivares ◽  
...  
Keyword(s):  
Vicia Faba ◽  
Human Lymphocyte ◽  
Cell Cycle Progression ◽  
Lymphocyte Culture ◽  
Root Tip ◽  
Genotoxic Effects ◽  
Animal Metabolism ◽  
Index Ratio

Abstract The aim of the study was to evaluate genotoxic effects of Pirimor-50®, a pirimicarb-based formulation (50 % active ingredient), in human lymphocyte cultures and Vicia faba root meristems. Furthermore, the objective was to examine a combined influence of insecticide treatment with mammalian microsomal S9 and vegetal S10 metabolic fractions or S10 mix metabolic transformation extracts (after Vicia faba primary roots treatment with Pirimor-50®). We used sister chromatid exchange assay-SCE and measured cell cycle progression and proliferation (proportion of M1-M3 metaphases and replication index ratio-RI). Two processes were used for plant promutagen activation: in vivo activation-Pirimor-50® was applied for 4 h to the plant and then S10 mix was added to lymphocytes; and, in vitro activation-lymphocytes were treated with Pirimor-50® and S10 or S9 for 2 h. Direct treatment induced significantly higher SCE frequencies in meristems at 0.01 mg mL-1. In lymphocytes, significantly higher SCE was at 1 mg mL-1 with decrease in RI and M1-M3 metaphase proportions at 0.5 mg mL-1 and cell division stop at 2.5 mg mL1. S10 mix lymphocyte treatment showed significantly elevated SCE values at 2-2.5 mg mL-1, with cell death at 3 mg mL-1. Lymphocyte treatment with Pirimor-50® together with S9 or S10 showed slightly elevated SCE frequency but had a significant influence on RI decrease, with lowest values in S9 treatment. Since no data are available on the genotoxicity of Pirimor-50®, this study is one of the first to evaluate and compare its direct effect in two bioassays, animal and vegetal, and also the effect of plant and animal metabolism on its genotoxic potential.

scholarly journals Does Caesalpinia bonducella ameliorate genotoxicity? An in vitro study in human lymphocyte culture and in vivo study in Albino mice

2013 ◽  
Vol 14 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Md. Sultan Ahmad ◽  
Sheeba Ahmad ◽  
Afsar Ali ◽  
Mohammad Afzal
Keyword(s):  
Human Lymphocyte ◽  
In Vitro Study ◽  
Lymphocyte Culture ◽  
Vitro Study ◽  
In Vivo Study ◽  
Caesalpinia Bonducella ◽  
Albino Mice

EFFECTS OF CYCLOPHOSPHAMIDE ON IN VITRO HUMAN LYMPHOCYTE CULTURE AND MITOGENIC STIMULATION

1983 ◽  
Vol 35 (2) ◽  
pp. 165-168 ◽  
Author(s):  
BRAHMA S. SHARMA
Keyword(s):  
Human Lymphocyte ◽  
Lymphocyte Culture ◽  
Mitogenic Stimulation

A histological study of the short-term effects of fluoride on enamel and dentine formation in hamster tooth-germs in organ culture in vitro

1984 ◽  
Vol 29 (10) ◽  
pp. 803-810 ◽  
Author(s):  
A.L.J.J. Bronckers ◽  
Lyda L. Jansen ◽  
J.H.M. Wöltgens
Keyword(s):  
Organ Culture ◽  
Histological Study ◽  
Short Term ◽  
Culture In Vitro ◽  
Tooth Germs ◽  
Short Term Effects

Study of the lymphocyte fractions in the mixed lymphocyte culture in vitro

1967 ◽  
Vol 23 (10) ◽  
pp. 848-849 ◽  
Author(s):  
D. C. Viza
Keyword(s):  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Culture In Vitro
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