Hepatitis B Viral Markers in the Human Milk of HBsAg-Positive Mothers: An Observational Study

Background: Quantification of viral antigens and viral loads in human milk samples from mothers infected with hepatitis B virus is largely unknown. Research Aim: The aim of the study was to quantitatively measure the levels of viral antigens and deoxyribonucleic acid of hepatitis B virus in human milk from mothers infected with hepatitis B virus. Methods: Fifty-five pairs of milk and serum samples from mothers with positive hepatitis B surface antigen, including 11 hepatitis B e antigen positive, were quantitatively tested to measure viral antigens by microparticle enzyme immunoassay and viral loads by real-time polymerase chain reaction assay. Results: The median level of hepatitis B surface antigen in the human milk samples of mothers with positive or negative hepatitis B e antigen was each lower than that in the sera, respectively (1.10 vs. 4.32 log10 IU/ml, t = 10.693, p < .001; -0.77 vs. 2.53 log10 IU/ml, t = -25.135, p < .001). The titers of hepatitis B surface antigen or hepatitis B e antigen in the human milk samples were each correlated with that in maternal serum. The detectable level of deoxyribonucleic acid of hepatitis B virus in human milk ranged from 1.42–5.27 log10 IU/ml, whereas that in maternal sera was 1.44–8.66 log10 IU/ml. The viral level in human milk was not correlated with that in maternal circulation. Conclusion: The present study data illustrate the relatively low titers of viral markers in the milk of mothers with positive hepatitis B surface antigen.

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Serum hepatitis B surface antigen quantification as a useful assessment for significant fibrosis in hepatitis B e antigen-positive hepatitis B virus carriers

Journal of Gastroenterology and Hepatology ◽

10.1111/jgh.12304 ◽

2013 ◽

Vol 28 (11) ◽

pp. 1746-1755 ◽

Cited By ~ 19

Author(s):

Yun-hao Xun ◽

Guo-qing Zang ◽

Jian-chun Guo ◽

Xiu-li Yu ◽

Hong Liu ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Serum Hepatitis ◽

Hepatitis B E Antigen ◽

Significant Fibrosis ◽

B Virus

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Hepatitis B Surface Antigen Levels Can Be Used to Rule Out Cirrhosis in Hepatitis B e Antigen-Positive Chronic Hepatitis B: Results From the SONIC-B Study

The Journal of Infectious Diseases ◽

10.1093/infdis/jiaa192 ◽

2020 ◽

Cited By ~ 1

Author(s):

Milan J Sonneveld ◽

Bettina E Hansen ◽

Willem P Brouwer ◽

Henry L-Y Chan ◽

Teerha Piratvisuth ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Chronic Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Hepatitis B E Antigen ◽

Significant Fibrosis ◽

B Virus ◽

Rule Out

Abstract Background Serum hepatitis B surface antigen (HBsAg) levels correlate with the duration of chronic hepatitis B virus (HBV) infection and may predict the extent of hepatic fibrosis. Methods We analyzed data from the SONIC-B database, which contains data from 8 global randomized trials and 2 large hepatology centers. Relationship between HBsAg levels and presence of significant fibrosis (Ishak 3–4) or cirrhosis (Ishak 5–6) were explored, and clinically relevant cutoffs were identified to rule out cirrhosis. Results The dataset included 2779 patients: 1866 hepatitis B e antigen (HBeAg)-positive; 322 with cirrhosis. Among HBeAg-positive patients, lower HBsAg levels were associated with higher rates of significant fibrosis (odds ratio [OR], 0.419; P < .001) and cirrhosis (OR, 0.435; P < .001). No relationship was observed among HBeAg-negative patients. Among HBeAg-positive patients, genotype-specific HBsAg cutoffs had excellent negative predictive values (>97%) and low misclassification rates (≤7.1%) and may therefore have utility in ruling out cirrhosis. Diagnostic performance of the HBsAg cutoffs was comparable among patients in whom cirrhosis could not be ruled out with fibrosis 4 (FIB-4). Conclusions Hepatitis B virus genotype-specific HBsAg cutoffs may have utility in ruling out presence of cirrhosis in HBeAg-positive patients with genotypes B, C, and D and can be an adjunct to FIB-4 to reduce the need for further testing.

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Possible transmission of hepatitis B virus between spouses and their children in Babol, Northern Iran

Tropical Doctor ◽

10.1258/004947507782332856 ◽

2007 ◽

Vol 37 (4) ◽

pp. 245-247 ◽

Cited By ~ 3

Author(s):

Mohammad Reza Hasanjani Roushan ◽

Minoo Mohraz ◽

Ali Akbar Velayati

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

Hepatitis B Surface Antigen ◽

Family Members ◽

Hbv Infection ◽

Hepatitis B E Antigen ◽

Northern Iran ◽

Hbv Markers ◽

B Virus

To determine the possible routes of transmission of hepatitis B virus (HBV) infection between spouses and their children in Babol, Northern Iran, the spouses of 54 infected husbands and 49 wives without any evidence of HBV infection in their family members were evaluated from March 1998 to April 2005. All of these cases were hepatitis B surface antigen and anti-hepatitis B 'e' antigen positive. Mean duration of marriage for husbands was 14.4±6.49 and for wives12.46±6.24 years. Past HBV infection was found in 46.3% wives of infected husbands and in 65.3% husbands of infected wives ( P = 0.074). HBV markers were seen in 32.5% children of infected fathers and 64.5% children of infected mothers ( P = 0.0001). HBV carrier rates in boys and girls of infected mothers were significantly higher than in those of infected fathers ( P = 0.002 and P = 0.0001, respectively). The results show that transmission of HBV between spouses and their children were the main routes of transmission of HBV in our region.

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HEPATITIS B VIRUS

The Professional Medical Journal ◽

10.29309/tpmj/2007.14.02.4883 ◽

2007 ◽

Vol 14 (02) ◽

pp. 241-247

Author(s):

JIANBO XIAO ◽

Lei Zhang ◽

XIAOQING CHEN ◽

Ming Xu * ◽

XINYU JIANG

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

Hepg2 Cells ◽

Morphological Changes ◽

Hepatitis B Surface Antigen ◽

Hbv Dna ◽

Hepatitis B E Antigen ◽

B Virus ◽

Clone Cells

Dried leaves of Marchantia convoluta are largely used to protect livers,and to treat tumefaction of skins in China. Flavonoids from Marchantia Convoluta (MCF) was one of the mostpotentially effective anti-inflammatory. MCF was studied here for its ability to inhibit the proliferation of 2,2,15 cells(clone cells derived from HepG2 cells that were transected with a plasmid containing HBV, DNA). All concentrations(5,10,20 and 40 :g/ml) of MCF inhibit hepatitis B surface antigen (HbsAg) and hepatitis B E antigen (HbeAg) in thecultured medium released from 2.2.15 cells. Analysis of morphological changes of MCF-treated phase- contrastmicroscope revealed a possible model of action for MCF to inhibit Proliferation of 2.2.15 cells by inducing apoptosis.

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