Chromosomal aberrations induced by imipramine and desipramine in mouse

2010 ◽  
Vol 29 (4) ◽  
pp. 297-302 ◽  
Author(s):  
E. Madrigal-Bujaidar ◽  
Y. Cárdenas García ◽  
I. Álvarez-González
Keyword(s):  
Chromosomal Aberrations ◽  
Bone Marrow Cells ◽  
Sister Chromatid Exchanges ◽  
Control Group ◽  
Mouse Bone Marrow ◽  
Chromosomal Damage ◽  
Drug Induced ◽  
Treatment Of Depression ◽  
Chromatid Exchanges

Imipramine (IMI) and desipramine (DES) are two drugs widely used for the treatment of depression as well as for other diseases. In the present study, we determined their capacity to induce chromosomal aberrations in mouse bone marrow cells. Three doses of each compound were tested and their results were compared with the frequency of chromosomal aberrations obtained in a control group as well as with a group treated with cyclophosphamide. Our results showed a significant increase in chromosome damage with the doses tested for each compound: 7, 20, and 60 mg/kg in the case of IMI, and 2, 20, and 60 mg/kg as regards DES. This last drug induced stronger chromosomal damage than IMI. Our results agree with previous studies regarding the induction of micronuclei and sister chromatid exchanges by the drugs in mouse and suggest caution with respect to their use in long-term treatments.

Chromosomal Aberrations and Sister Chromatid Exchanges in Lymphocytes and Urine Mutagenicity of Migraine Patients: A Comparison of Chronic Feverfew Users and Matched Non-users

1988 ◽  
Vol 7 (2) ◽  
pp. 145-152 ◽  
Author(s):  
D. Anderson ◽  
P.C. Jenkinson ◽  
R.S. Dewdney ◽  
S.D. Blowers ◽  
E.S. Johnson ◽  
...  
Keyword(s):  
Chromosomal Aberrations ◽  
Sister Chromatid ◽  
Test System ◽  
Sister Chromatid Exchanges ◽  
Control Group ◽  
Matched Pairs ◽  
User Group ◽  
Mean Frequency ◽  
The Mean ◽  
Chromatid Exchanges

1 Thirty migraine patients who had taken the leaves, tablets or capsules of feverfew daily for more than 11 consecutive months were compared to 30 feverfew non-user migraine patients who had been individually age- and sex-matched. 2 The frequency of chromosomal aberrations and sister chromatid exchanges (SCE) were determined from lymphocyte cultures established from blood samples taken over a period of several months. Matched pairs were sampled on the same date for two-thirds of the cases, and the greatest difference in sampling time of the remainder was 20 days. Also, the mutagenicity of urine samples from 10 feverfew user migraine patients was compared to that from 10 matched non-user migraine patients using the Ames Salmonella mutagenicity test system. Paired samples were given on the same date. 3 The mean frequency of chromosomal aberrations in the feverfew user group was lower than that in the non-user group both in terms of cells with breaks (2.13% vs 2.76%) and in terms of cells with all aberrations (4.34% vs 5.11%). However, this difference was small and not significant. 4 The mean frequency of SCE in the feverfew exposed group was lower than that in the control group (8.78 vs 8.80 SCE/cell), but, this difference was not significant as determined by factorial analysis of variance (P = 0.897). There was a highly significant variance between the frequencies of SCE in the matched pairs of migraine patients but this was not related to age, sex or feverfew exposure. 5 The mean number of revertants in the Ames mutagenicity assay was greater for the urine of the feverfew user migraine patients than that of the non-user migraine patients, in both strains of bacteria, with or without the inclusion of an S-9 metabolizing system. However, the increases were small and not significant. 6 The data indicate that the prophylactic use of feverfew for the alleviation of migraine symptoms affects neither the frequency of chromosomal aberrations nor the frequency of SCE in the circulating peripheral lymphocytes. Also, the mutagenicity of urine from feverfew user migraine patients is unaffected compared to urine from non-user migraine patients detectable by the methods used in this study.

scholarly journals Genotoxic and Cytotoxic Studies of Beta-Sitosterol and Pteropodine in Mouse

2005 ◽  
Vol 2005 (3) ◽  
pp. 242-247 ◽  
Author(s):  
R. Paniagua-Pérez ◽  
E. Madrigal-Bujaidar ◽  
S. Reyes-Cadena ◽  
D. Molina-Jasso ◽  
J. Pérez Gallaga ◽  
...  
Keyword(s):  
Cellular Proliferation ◽  
Bone Marrow Cells ◽  
Safety Evaluation ◽  
Sister Chromatid Exchanges ◽  
Mouse Bone Marrow ◽  
Pharmacological Activities ◽  
Polychromatic Erythrocytes ◽  
Cytotoxic Studies ◽  
Chromatid Exchanges ◽  
The Relationship

Beta-sitosterol (BS) and pteropodine (PT) are constituents of various plants with pharmacological activities potentially useful to man. The chemicals themselves possess biomedical properties related to the modulation of the immune and the nervous systems, as well as to the inflammatory process. Therefore, safety evaluation of the compounds is necessary in regard to their probable beneficial use in human health. The present study evaluates their genotoxic and cytotoxic potential by determining the capacity of the compounds to induce sister chromatid exchanges (SCE), or to alter cellular proliferation kinetics (CPK) and the mitotic index (MI) in mouse bone marrow cells. Besides, it also determines their capacity to increase the rate of micronucleated polychromatic erythrocytes (MNPE) in peripheral mouse blood, and the relationship polychromatic erythrocytes/normochromatic erythrocytes (PE/NE) as an index of cytotoxicity. For the first assay, four doses of each compound were tested: 200, 400, 600, and 1000 mg/kg in case of BS, and 100, 200, 300, and 600 mg/kg for PT. The results in regard to both agents showed no SCE increase induced by any of the tested doses, as well as no alteration in the CPK, or in the MI. With respect to the second assay, the results obtained with the two agents were also negative for both the MNPE and the PE/NE index along the daily evaluation made for four days. In the present study, the highest tested dose corresponded to 80% of the LD50obtained for BS and to 78% in the case of PT. The results obtained establish that the studied agents have neither genotoxic nor cytotoxic effect on the model used, and therefore they encourage studies on their pharmacological properties.

Sister chromatid exchanges produced by imipramine and desipramine in mouse bone marrow cells treated in vivo

2002 ◽  
Vol 132 (2) ◽  
pp. 123-129 ◽  
Author(s):  
R. Paniagua-Pérez ◽  
E. Madrigal-Bujaidar ◽  
C.S. Reyes ◽  
G.J. Pérez ◽  
M.O. Velasco ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Sister Chromatid ◽  
Bone Marrow Cells ◽  
Sister Chromatid Exchanges ◽  
Mouse Bone Marrow ◽  
Chromatid Exchanges ◽  
Mouse Bone Marrow Cells ◽  
Marrow Cells
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