Assessment of the neutralizing potency of antisera raised against native and γ-irradiated Naja nigricollis (black-necked spitting cobra) venom in rabbits, concerning its cardiotoxic effect

2017 ◽  
Vol 36 (12) ◽  
pp. 1335-1344 ◽  
Author(s):  
F Abdou ◽  
EE Denshary ◽  
E Shaaban ◽  
M Mohamed
Keyword(s):  
Creatine Kinase ◽  
Lactate Dehydrogenase Level ◽  
Active Immunization ◽  
Significant Inhibition ◽  
The Other ◽  
Significant Elevation ◽  
Creatine Kinase Isoenzyme ◽  
Cardiotoxic Effect ◽  
Creatine Kinase Isoenzyme Mb

The present study was designed to prepare a specific safe antiserum for Naja nigricollis using γ-irradiated (1.5KGy and3KGy) venoms. Rabbits were used for active immunization using irradiated venoms (1.5 and 3 kGy) as a toxoid, mice were used for determination of LD50 post immunization and the rats were used for neutralization of the cardiotoxic effect of venom. Results of the immunodiffusion test indicated that the sera of rabbits raised against non-irradiated, 1.5- and 3-kGy γ-irradiated venom, had the same results of precipitin bands. A significant inhibition of phospholipase A2 activities was obtained when neutralized with native, γ-irradiated (1.5KGy and3KGy) venoms. On the other hand, preincubation of the venom ½ LD50 (0.154 mg/kg i.p.) with each antiserum (non-irradiated or irradiated venom) at 37°C for 1 h in a ratio (1:4) produced a significant reduction in the values of creatine kinase and creatine kinase isoenzyme-MB. However, significant elevation in aspartate aminotransferase level and no change in lactate dehydrogenase level were observed. So the results of this study indicated that the irradiated venom treatment reduces the cardiotoxic effect of venom in immunized immunization animals for preparing vaccines.

Clinical evaluation of an immunoinhibition procedure for creatine kinase-MB.

1980 ◽  
Vol 26 (1) ◽  
pp. 150-152
Author(s):  
D Obzansky ◽  
J A Lott
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Diagnostic Sensitivity And Specificity ◽  
Creatine Kinase Isoenzyme Mb

Abstract We have clinically evaluated the Dade "Cardiozyme" immunoinhibition procedure for determination of creatine kinase isoenzyme MB (CK-MG) in 71 patients who were suspected of having had an acute myocardial infarction. Electrophoresis for CK-MB was also carried out. On the basis of diagnostic sensitivity and specificity for myocardial infarction, we found the Dade procedure for CK-MB to be somewhat inferior to electrophoresis. In 11 patients for whom the time of infarction was known, we observed normal CK-MB results for two of them by both immunoinhibition and electrophoresis during the first 24 h, but subsequently could detect abnormal CK-MB results by both methods. Thus in some patients such data are not helpful for making a diagnosis in the first 24 h. The Dade procedure is easy to perform, but lacks sensitivity in the region of low CK-MB activity, requires a very stable spectrophotometer, is imprecise, and produces negative numerical results in patients without myocardial infarction.

Clinical evaluation of an immunoinhibition procedure for creatine kinase-MB.

1980 ◽  
Vol 26 (1) ◽  
pp. 150-152 ◽  
Author(s):  
D Obzansky ◽  
J A Lott
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Sensitivity And Specificity ◽  
Clinical Evaluation ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Diagnostic Sensitivity And Specificity ◽  
Creatine Kinase Isoenzyme Mb

Abstract We have clinically evaluated the Dade "Cardiozyme" immunoinhibition procedure for determination of creatine kinase isoenzyme MB (CK-MG) in 71 patients who were suspected of having had an acute myocardial infarction. Electrophoresis for CK-MB was also carried out. On the basis of diagnostic sensitivity and specificity for myocardial infarction, we found the Dade procedure for CK-MB to be somewhat inferior to electrophoresis. In 11 patients for whom the time of infarction was known, we observed normal CK-MB results for two of them by both immunoinhibition and electrophoresis during the first 24 h, but subsequently could detect abnormal CK-MB results by both methods. Thus in some patients such data are not helpful for making a diagnosis in the first 24 h. The Dade procedure is easy to perform, but lacks sensitivity in the region of low CK-MB activity, requires a very stable spectrophotometer, is imprecise, and produces negative numerical results in patients without myocardial infarction.

Assay of creatine kinase isoenzyme MB in serum with time-resolved immunofluorometry

1990 ◽  
Vol 36 (9) ◽  
pp. 1679-1683 ◽  
Author(s):  
E S Lianidou ◽  
T K Christopoulos ◽  
E P Diamandis
Keyword(s):  
Creatine Kinase ◽  
Laser Excitation ◽  
Solid Phase ◽  
Time Resolved ◽  
Creatine Kinase Isoenzyme ◽  
Phase Complex ◽  
Precision And Accuracy ◽  
Accuracy Indices ◽  
First Time ◽  
Creatine Kinase Isoenzyme Mb

Abstract We describe the first time-resolved immunofluorometric assay for creatine kinase (EC 2.7.3.2) isoenzyme MB (CK-MB) in serum. The assay is based on the formation of the complex: solid-phase anti-CK-MB-CK-MB-biotinylated anti-CK-BB-streptavidin-BCPDA-Eu3+, where anti-CK-MB and anti-CK-BB are monoclonal antibodies against the CK isoenzymes MB and BB, respectively, and BCPDA is the europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid. The solid-phase complex is fluorescent and is measured on the dry solid-phase (microtiter well) in a specially designed time-resolved fluorometer that uses laser excitation. The assay requires 25 microL of serum and is not affected by the presence of either CK-MM (up to 5000 micrograms/L) or CK-BB (up to 1000 micrograms/L) in the sample. Precision and accuracy indices for the assay were satisfactory.

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