Defective neutrophil function in workers occupationally exposed to hexachlorobenzene

1997 ◽  
Vol 16 (6) ◽  
pp. 322-326 ◽  
Author(s):  
Mls Queiroz ◽  
C. Bincoletto ◽  
Rcr Perlingeiro ◽  
CA Souza ◽  
H. Toledo
Keyword(s):  
Respiratory Burst ◽  
Polymorphonuclear Leukocytes ◽  
Neutrophil Function ◽  
Nitroblue Tetrazolium ◽  
Chlorinated Compounds ◽  
Blood Concentrations ◽  
Nitroblue Tetrazolium Reduction ◽  
Occupationally Exposed ◽  
Tetrazolium Reduction ◽  
Age And Sex

In this work we have studied the respiratory burst and chemotaxis of polymorphonuclear leukocytes from 51 workers exposed to chlorinated compounds, which were compared with those of non-exposed, age- and sex- matched invididuals. These two neutrophil functions were significantly reduced as compared to controls. No correlation was observed between the length of exposure, hexachlorobenzene (HCB) blood concentrations and neu trophil chemotaxis or the extent of nitroblue tetrazolium reduction.

scholarly journals The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 309-313 ◽  
Author(s):  
RL Baehner ◽  
LA Boxer ◽  
J Davis
Keyword(s):  
Chronic Granulomatous Disease ◽  
Polymorphonuclear Leukocytes ◽  
Nitroblue Tetrazolium ◽  
Granulomatous Disease ◽  
Biochemical Basis ◽  
Nitroblue Tetrazolium Reduction ◽  
Normal Human ◽  
Nbt Reduction ◽  
Human Polymorphonuclear Leukocytes ◽  
Tetrazolium Reduction

Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1–14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

scholarly journals Effect of smoking on neutrophil oxidative metabolism

Medicina ◽  
2007 ◽  
Vol 44 (3) ◽  
pp. 195
Author(s):  
Darius Zasimauskas ◽  
Gediminas Žekonis
Keyword(s):  
Oxidative Metabolism ◽  
Periodontal Diseases ◽  
Neutrophil Function ◽  
Nitroblue Tetrazolium ◽  
Systemic Diseases ◽  
Nitroblue Tetrazolium Reduction ◽  
Smoking Exposure ◽  
Before And After ◽  
Nitroblue Tetrazolium Test ◽  
Tetrazolium Reduction

Alterations in neutrophil function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking-related systemic diseases. The aim of the study was to evaluate the effect of smoking on neutrophil oxidative metabolism. Materials and methods. The study included 17 smoking men free of systemic diseases who were referred for treatment of various odontological diseases to outpatient department of Kaunas University of Medicine Hospital. The age of subjects varied from 22 to 43 years. All subjects answered the questions about smoking habits. Clinical examination included assessment of oral hygiene status according to the OHI-s index and periodontal status according to Russell and Ramfjord indices. To evaluate the oxidative metabolism of neutrophils, luminol- and liucigenin-dependent chemiluminescence and nitroblue tetrazolium test were used. Results. After smoking, extracellular liucigenin-dependent chemiluminescence response was higher as compared to the response before smoking, but total (intra- and extracellular) luminol-dependent chemiluminescence response was the same both before and after smoking. Exposure of neutrophils to smoking caused a significant increase in nitroblue tetrazolium reduction. Conclusion. The release of reactive oxygen species in neutrophils exposed to smoking may alter the pathogenic processes in periodontal diseases.

scholarly journals The biochemical basis of nitroblue tetrazolium reduction in normal human and chronic granulomatous disease polymorphonuclear leukocytes

Blood ◽  
1976 ◽  
Vol 48 (2) ◽  
pp. 309-313 ◽  
Author(s):  
RL Baehner ◽  
LA Boxer ◽  
J Davis
Keyword(s):  
Chronic Granulomatous Disease ◽  
Polymorphonuclear Leukocytes ◽  
Nitroblue Tetrazolium ◽  
Granulomatous Disease ◽  
Biochemical Basis ◽  
Nitroblue Tetrazolium Reduction ◽  
Normal Human ◽  
Nbt Reduction ◽  
Human Polymorphonuclear Leukocytes ◽  
Tetrazolium Reduction

Abstract Normal human polymorphonuclear leukocytes (PMN) placed in anaerobic chambers reaching pO2's of less than 5 mm Hg fail to generate O2-, iodinate ingested particles, and stimulate glucose-1–14C oxidation through the hexose monophosphate shunt. The observation that anaerobic cells are incapable of generating O2- or reducing nitroblue tetrazolium (NBT) to formazan supports the idea that NBT reduction in phagocytizing PMN is due exclusively to oxygen-dependent O2- generating oxidase which is deficient in chronic granulomatous disease leukocytes, despite their hyperphagocytic capacity.

scholarly journals Nitroblue Tetrazolium Reduction: False Positive and False Negative Results

Blood ◽  
1973 ◽  
Vol 41 (6) ◽  
pp. 921-925 ◽  
Author(s):  
Philip Ashburn ◽  
M. Robert Cooper ◽  
Charles E. McCall ◽  
Lawrence R. DeChatelet
Keyword(s):  
Bacterial Infection ◽  
Polycythemia Vera ◽  
False Positive ◽  
False Negative ◽  
Nitroblue Tetrazolium ◽  
Chronic Granulocytic Leukemia ◽  
Nitroblue Tetrazolium Reduction ◽  
Nbt Test ◽  
Granulocytic Leukemia ◽  
Tetrazolium Reduction

Abstract Quantitative and histochemical nitroblue tetrazolium reduction (NBT) tests were performed on leukocytes of 13 patients with polycythemia vera, seven with chronic granulocytic leukemia, eight with neoplastic disease associated with fever, 16 with bacterial infection, and 13 healthy control individuals. No significant differences were detected in the quantitative test between any of the groups studied. The histochemical NBT test was significantly higher than control in the patients with polycythemia vera and neoplasia associated with fever, as well as in those with known bacterial infection. It is suggested that patients with neoplasia or polycythemia vera may show a false positive reaction in the histochemical NBT test. Conversely, the test was significantly lower than control in patients with chronic granulocytic leukemia. Such patients might show a false negative reaction in the test even if infection were present.

scholarly journals Effect of dihydroheptaprenol on nitroblue tetrazolium reduction by swine alveolar macrophages.

1989 ◽  
Vol 51 (3) ◽  
pp. 630-631
Author(s):  
Toshihiro WATARI ◽  
Ryo GOITSUKA ◽  
Hidekazu KOYAMA ◽  
Toshinori SAKO ◽  
Tomiya UCHINO ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Nitroblue Tetrazolium ◽  
Nitroblue Tetrazolium Reduction ◽  
Tetrazolium Reduction
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