Intracellular location matters: rationalization of the anti-inflammatory activity of a manganese(ii) superoxide dismutase mimic complex

The study of Mn-based superoxide dismutase mimic conjugated with a multimodal Re-probe in a cellular model of oxidative stress revealed that its bioactivity is associated with its accumulation at the mitochondria.

Anti-inflammatory activity of superoxide dismutase mimics functionalized with cell-penetrating peptides

A superoxide dismutase mimic was functionalized with three peptides: -R9, -RRWWRRWRR or -Fx-r-Fx-K (MPP). They were studied in intestinal epithelial cells in an inorganic cellular chemistry approach: quantification, distribution and bio-activity.

Activity of Superoxide Dismutase Mimic of [Mn(salen)OAc] Complex Compound Non-enzymatically in Vitro Through Riboflavin Photoreduction

The complex compound of [Mn(salen)OAc] can serve as mSOD and its activity has been determined non-enzymatically in vitro through riboflavin photoreduction. The complex was synthesized from Mn(OAc)2.4H2O and H2salen. Based on the elemental analysis, the C=56.69%; H=4.21%; and N=7.52% content are corresponding to the chemical formula of MnC18H17N2O4. The functional groups and ionic species in the complex have been analyzed by infrared spectroscopy and ESI-MS. SOD activity was determined by mixing complex at various concentrations with riboflavin and nitroblue tetrazolium (NBT), then the mixture was lighted with 20 Watt tungsten lamp for 15 minutes in a closed box. Then the reduced NBT absorptions were measured at λ 560 nm. The difference of absorbance between standard and sample solutions (without and with riboflavin, respectively) was multiplied by 100% to obtain %inhibition of each various sample concentration against NBT. SOD activity was obtained from IC50 data defined as a 50% inhibition of the plot curve of % inhibition to the concentration of the complex. The result obtained for this compound is IC50 = 2.7 ± 0.05 µM as well as enzymatic method. Therefore, this method can be used to determine the SOD activity by giving more stability and accuracy of IC50 value.